Archives of Pharmacal Research
- Volume 22 Issue 3
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- Pages.232-236
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- 1999
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Aloesin and Arbutin Inhibit Typrosinase Activity in a Synergistic Manner via a Different Action Mechanism
- Jin, Ying-Hua (College of Pharmacy, Seoul National University) ;
- Lee, Suk-Jin (College of Pharmacy, Seoul National University) ;
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Chung, Myung-Hee
(Colleg of Medicine Seoul National University) ;
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Park, Jeong-Hill
(College of Pharmacy, Seoul National University) ;
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Park, Young-In
(College of Natural Resources, Korea University) ;
- Cho, Tae-Hyeong (Namyang Aloe Bio-science Research Institute) ;
- Lee, Seung-Ki (College of Pharmacy, Seoul National University)
- Published : 1999.06.01
Abstract
In this study, we present evidence that cotreatment of aloesin and arbutin inhibits tyrosinase activity in a synergistic manner by acting through a different action mechanism. Aloesin or arbutin similarly inhibited enzyme activity of human- and mushroom-tyrosinases with an IC50 value of 0.1 or 0.04 mM, respectively. Lineweaver-Burk plots of the enzyme kinetics data showed that aloesin inhibited tyrosinase activity noncompetitively with a Ki value of 5.3 mM, whereas arbutin did it competitively (Maeda, 1996). We then examined whether cotreatment of these agents inhibits the tyrosinase activity in a synergistic manner. The results showed that 0.01 mM aloesin in the presence of 0.03 mM arbutin inhibited activity of mushroom by 80% of the control value and the reverse was also true. The inhibitory effects were calculated to be synergistic according to the B rgi method. Taken together, we suggest that aloesin along with arbutin inhibits in synergy melanin production by combined mechanisms of noncompetitive and competitive inhibitions of tyrosinase activity.