Inhibitory Effects of Water-Acetone Extracts of Chestnut inner shell, Pine needle and Hop on The Melanin Biosynthesis

율피.솔잎.호프의 수성 아세톤 추출물에 의한 Melanin 생성 억제 효과

  • 양민진 (동덕여자대학교 보건관리학과) ;
  • 김명길 (경기도 보건환경연구원) ;
  • 임세진 (동덕여자대학교 약하과) ;
  • 안형수 (동덕여자대학교 약하과) ;
  • 안령미 (동덕여자대학교 보건관리학과)
  • Published : 1999.08.01


The skin whitening effects of pine needle extract, hop extract and chestnut inner shell extract were evaluated both in vitro and in B 16 mouse melanoma cell lines. Each extracts significantly inhibited tyrosinase activity, dopa auto-oxidation and melanin biosynthesis in vitro and in B 16 cell lines. In vitro, hop extract inhibited melanin biosynthesis 15 times stronger than kojic acid at $10{\;}\mu\textrm{g}/ml$ concentration. Each extracts were stronger inhibitors of melanin biosynthesis than kojic acid in B 16 mouse melanoma cell at less than $4{\;}\mu\textrm{g}/ml$ concentration. These results show that extracts fo pine needle, hop and chestnut inner shell could be developed as skin whitening component of cosmetics.


  1. J. Invest. Dermatol v.195 The relationship between gyrosinase activity and skin color in human forskins Iwata, M.;Corn T.;Iwata, S.;Evertte, M.;Fuller, B. B.
  2. Photochem. Photobiol v.65 no.3 Induced melanin reduces mutation and cell killing in couse melanoma Weixiong, Le.;Helene, Z. Hill
  3. Meth. In. Enzymol v.205 Vectors used for expression in mammalian cells Kaufman, R. J.
  4. J. Invest. Dermatol v.100 Pigment production in murine melanoma cells is regulated by tyrosinase, tyrosinase-related preotein 1(TRP 1), dopachrame tautomerase(TRP 2) and a melanogenic inhibitor Kameyama, K.; Takemura, T.;Hamada, Y.;Sakai, C.;Kondoh, S.;Nishiyama, S.
  5. J. Soc. Cosmen. Chem v.42 In vitro effectiveness of sdveral whitening cosmetic components in human melanocyte Maeda, K.;Fukuda, M.
  6. J. Biol. Chem v.172 The chemistry of melanin Mason, H. S.
  7. Biol. Pharm. Bull v.19 no.1 Studies of cuticle drugs from natural souces. IV. Inhibitory effect of some arctostaphylos plants on melanin biosynthesis Hideaki, M.;Masayuki, H.;Weizhong, C.;Hideki, T.;Munekazu, I.;Michinori, K.
  8. J. Immunol. Methods v.65 Rapid colorimetric assay for cellular growth and survival ; Application to proliferation and cytotoxicity assays,. Mosmann, T.
  9. J. Dermatol v.126 Effects of staurosporine, PMA and A23187 on human melanocyte cultures with dibutyryl cyclic AMP. Br Maeda, K.;Tomita, Y.;Fukuda, M.;Tagami, H.
  10. J. Invest. Dermatol v.125 Pigment contment of cultured human melanocyte dose not correlate with tyrosinase messeage leve Gordon, P. R.;Naeyaert, J. M.;Eller, M.;Park, H. Y.;Gilchrest, B. A.
  11. Acta-Derm-Venereol v.60 no.3 Morphology of epidermal melanocytes in different stages of mitosis Rosdahl, IK.;Lindstrom, S.
  12. Arct. Inst. Cardiol. Mex v.54 no.4 Hypotensive effect of dopaminergic agonists in the rat Prieto, J. C.;Quevedo, M.
  13. 식물자원의 폴리페놀류 분류와 기능성 신소재 기술개발 보고서
  14. 율피를 팩으로 이용한 계절별 피부고습효과에대한 연구 윤원자
  15. 율피 추출물에 의한 tyrosinase 활성억제 및 멜라닌 생성억제 효과 양민진
  16. Biol. Pharm. Bull v.18 no.8 Studies of cuticle drugs from natural sources. Ⅲ. Inhibitory effect of myrica ruvra on melanin biosynthesis Hydeaki, M.;Masayuki, H.;Weizhong, C.;Hedeki, T.;Munekazu, I.;Michinori, K.
  17. J. Dermatol. Sci v.2 Linoleic acid can induce depigmentation in cultured normal human melanocytes during activtiy proliferating sate Ando, H.;Horikawa, T.;Oka, M.
  18. Cancer Res v.40 Stimulation of melanogenesis in human melanoma cell line by retinoids Reuben, Lotan;Dafna, Lotan
  19. J. Invest. Dermatol v.100 Regulation mechanism of melanogenesis beyond the tyrosinase concept Prota, G.
  20. Pigment Cell Res v.11 Effect of arbutin on melanogenic proteins in human melanocytes Ashok. K.;Yoko, funasaka;Mari, Komoto.