Partial Purification and Stability of a Water-soluble Yellow Pigment from Bacillus sp. PY123.

Bacillus sp. PY123 균주가 생성하는 수용성 황색수소의 부분 정제 및 그 안정성

  • 김지연 (동의대학교 기초과학연구소) ;
  • 김광현 (동의대하교 미생물학과) ;
  • 김병우 (동의대학교 미생물학과) ;
  • 이광배 (대구보건대학 보건위생과)
  • Published : 1999.12.01

Abstract

For application of a yellow pigment as food additives, stability of a water-soluble yellow pigment from Bacillus sp. PY123 was investigated. The yellow pigment from Bacillus sp. PY123 was purified with pH treatment, activated carbon and silica gel column chromatography. The partial purified yellow pigment appeared only one spot on silica gel TLC after 12 evaporation and under irradiation of UV 253nm at dark room. Rf value of the pigment was measured at 0.04 and 0.12 with development of a solvent mixture (Butanol : Acetic acid : water = 4 :1:5) and a solvent mixture (Isopropanol : Ammonia : Water = 9 :1: 2), respectively, The partial purified pigment appeared a white fluorescence under UV365nm irradiation. The partial purified yellow pigment had a main peak and a minor peak on HPLC using 20mM phosphate buffer(pH 7.0) at 1ml/min flow rate. The partial purified pigment was stable at heat treatment, acidic pH, oxide-reductants and surfactants.

Keywords