Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Patterns of Utilizing Sole Carbon Source by Soil Microbes in a Forest Soil

토양 세균 군집의 유일탄소원 이용에 의한 지문분석

  • 송인근 (한양대 생물학과, 서울대 분자미생물학연구센터) ;
  • 최영길 (한양대 생물학과, 서울대 분자미생물학연구센터) ;
  • 안영범 (서울대 분자미생물학연구센터) ;
  • 신규철 (한양대 생물학과) ;
  • 조홍범 (서경대 생물공학과)
  • Published : 1999.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was carried out utilzing ability of sole carbon sources in soil microbial communities used by Biolog GN microplate. Cluster analysis showed that soil microbial cornmuties were categorized into three groups as forest, non-forest soil and naked soil of microbial group. Soil microbial commutites in a forest soil of Qirercus mongoIica was divided into another group microbial communites in Qirercus dendata vegetation soil and Pinus dnzsqlora vegetation soil by Multidimensional scaling(MDS). Generally, sole carbon utilzing abilties were higher in order of polymer, amino acids and carboxylic acids, but it was lower in amides substrates carbon group. From the result: it was supposed that metabolic diversity of microbial communities was corresponded to vegetation succession.

BIOLOG GN microplate를 이용하여 6종의 식생 토양에 따른 토양미생물 군집의 유일탄소원 이용능을 비교 분석하였다. 전체적으로 축합물, 아미노산, 카르복실산 화합물을 잘 이용하였으나, amide의 경우 낮은 이용능을 보였다. 대응분석을 통해 6종류의 식생 토양에 따른 토양미생물 군집은 크게 삼림토양, 초지-경작지토양, 비식생 토양의 3가지 그룹의 대사적 다양성의 차이를 보이는 집단으로 분류되었다. 다차원 척도법 분석을 통해 신갈나무 삼림토양의 미생물 군집은 소나무와 떡갈나무 삼림토양의 미생물 군집과는 또 다른 그룹으로 분리되었다. 이러한 식생토양형에 다른 미생물 군집의 대사 다양성의 차이로 볼 때, 식생의천이에 따라 식생에 영향을 받는 미생물 군집의 대사 다양성 또한 식생의 천이에 대응하여 변화하고 있음을 확인하였다.

Keywords

References

  1. 수계 세균 군집의 구조와 기능에 미치는 산성화 영향 모사 분석 안영범
  2. Appl. Environ. Microbiol. v.58 Soil bacterial biomass, activity, phospholipid fatty acid pattern, and pH tolerance in area polluted with alkaline dust deposition Baath, E.;A. Frostegard;H. Fritze
  3. Adv. Microb. Ecol. v.7 Diversity of microbial communities Atlas, R. M.
  4. Biol. Fertil. Soils v.16 Suitability of dehydrogenase activity assay as an index of soil biological activity Bayer, L.;C. Wachendorf;D.C. Elsner;R. Knabe
  5. Appl. Environ. Microbiol. v.61 Impact of carbon and flooding in the metabolic diversity of microbial communities in soils Bossio, D. A.;K. M. Scow.
  6. J. Microbiol. Methods v.30 Use of rhizosphere carbon sources in sole carbon source tests to discriminate soil microbial communties Campbell, D. D.;S. J. Grayston;D. J. Hirst.
  7. FEMS Microbiol. Ecol. v.16 Metabolic profiling as a means of characterizing plant-associated microbial communities Ellis, R. J.;P. Thompson;M. J. Bailey
  8. Appl. Environ. Microbiol. v.57 Classification and characterization of heterotrophic microbial communities on the basis of patterns of community-level soli-carbon-source utilization Garland, J.L.;A. L. Mills
  9. Beyond the Biomass A community-level physiological approach for studying microbial communities Garland, J.L.;A.L. Mills;K. Ritz;J. Dighton;K.E. Giller(eds.)
  10. Appl. Environ. Microbiol. v.61 Analysis factors affecting the accuracy, reproducibility, and interpletation of microbial community carbon source utilization patterns Haack, S.K.;H. Garchow;M.J. Klug;L.J.Forney
  11. J. Microbiol. Methods v.30 Evaluation of community-level catabolic profiling using BIOLOG GN microplates to study microbial community changes in potato phyllosphere Heuer, H.;K. Smalla
  12. J. Microbiol. Methods v.30 Application of muitivariance and related techniques in soil studies with substrate utilization tests Hitzl, W.;M. Henrich;M. Kessel;H. Insam
  13. Appl. Environ. Microbiol. v.33 Use of nucleopore filters for counting bacteria by fluorescene microscopy Hobbie, J. E.;R. J. Daley;S. Jasper
  14. Microbial. Ecol. v.31 Changes in functional abilities of the microbial community during composting of manure Insam, H.;K. Amour;M. Renner;C. Cepaz
  15. Microb. Ecol. v.13 Viability of soil bacteria: Optimisation of plate-counting technique and comparison between total counts and and plate counts within different size groups. Olsen, R. A.;L. R. Bakken
  16. SAS User's Guide: Stastics SAS Institute, Inc.
  17. Microbial Communities-functional and structural approaches Different carbon source utilization profiles of four tropical soils from Ethiopia Sharam, S.;A. Piccolo;H. Insam;H. Insam;H. Rangger(eds.)
  18. Environ. Rev. v.5 Conservation of forest soil microbial diversity: the impact of fire and research needs Staddon, W. J.;L. C. Deuchesne;J. T. Trevors
  19. Beyond the Biomass Fingerprinting bacterial soil communities using BIOLOG microtitre plates Winding, A. K.;K. Ritz;J. Dighton;K. E. Giller(eds.)
  20. Environ. Manage. v.21 Effects of recreational impacts on soil microbial communities Zabinski, C. A. J. E. Gannon
  21. Soil Biol. Biochem. v.26 Functional diversity of microbial communities: a quantitative approach Zak, C. J.;M. R. Willing;D. L. Moorhead;H. J. Wildman