Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Comparison of metabolic diversity by sole carbon source utilization and genetic diversity by restriction patterns of amplified 16S rDNA (ARDRA)in soil bacterial communities.

토양세균 군집의 대사 다양성과 16S rDNA의 제한효소 지문분석에 의한 유전적 다양성의 비교

  • 송인근 (한양대 생물학과, 서울대 분자미생물학연구센터) ;
  • 최영길 (한양대 생물학과, 서울대 분자미생물학연구센터) ;
  • 김유영 (한양대 생물학과) ;
  • 조홍범 (서경대 생물공학과)
  • Published : 1999.03.01
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Abstract

To investigate soil bacterial diversity according to vegelalioo types, utilizing ability of sole carbon sources and restriction enzyme patterns of 16s rDNA were analyzed. From the both results; five kinds of soil microbial communities were grouped as forest soil (Quercus mongolica and Pinus densi&ra vegetation), grass-agricultured soil and microbial communities of naked soil. But, both soil microbial communities of directily exlracted from ths soil and indirectly extracted from heterotrophic bacteria that cultured soil in LB medium showed very different similarity.

BIOLOG GN microplate를 이용한 유일탄소원의 이용능 비교를 통한 대사적 유사성과 16S rDNA 의 PCR 증폭산물의 제한효소 지문 분석에 따른 유전적 유사성을 5종의 식생토양에 따른 토양미생물 군집을 대상으로 비교하였다. 16S rDNA를 증폭하여 제한효소 지문을 분석한 결과, 토양으로부터 직접 추출하여 증폭한 토양세균 군집의 16S rDNA의 유전적 유사도는 BIOLOG GN microplate를 이용한 대사적 구조와 일치하는 경향을 보았다. 그러나 배양된 종속영양세균 군집의 다양성과는 유전적 유사도가 매우 낮게 나타났다.

Keywords

References

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