감두약침액의 암세포 성장 저해 효과

Antitumor Activity of Gamdutang Aqua-Acupuncture Solution

  • 조경희 (동국대학교 한의학대학 경혈학교실) ;
  • 한상훈 (동국대학교 한의학대학 경혈학교실) ;
  • 임종국 (동국대학교 한의학대학 경혈학교실) ;
  • 손윤희 (의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 이임태 (의과대학 약리학교실 및 난치병한양방치료연구센터) ;
  • 남경수 (의과대학 약리학교실 및 난치병한양방치료연구센터)
  • 발행 : 1999.12.01

초록

Gamdutang aqua-acupuncture solution (GAS), Gamdutang water-extracted solution (GWS) and Dae-Gamdutang aqua-acupuncture solution (DGAS) were prepared and tested for antitumor activities. It was shown to possess considerable toxicity toward various tumor cell lines. Concentration of 5 $\times$ and 10$\times$ of GAS resulted in more than 70% inhibition of growth in Ehrlich ascites tumor cells (EATC), Hepa1c1c7 and A549. GAS at concentrations of 5$\times$ and 10$\times$ revealed more than 60% inhibition in HeLa. GWS showed more than 50% inhibition of growth with EATC and HeLa at concentrations of 5$\times$ and 10$\times$, respectively. Toxicity assay with GWS in Hepa1c1c7 and A549 revealed that more than 80% inhibition of growth at the concentration of 5$\times$ and 10$\times$. In morphological study, the number of cells were decreased, and the shape of cells was round-form in EATC, Hepa1c1c7, A549, HeLa with GAS.

키워드

참고문헌

  1. 臨床配合本草學 康秉秀
  2. 생약학회지 v.30 금은화 약침액의 암예방 효과 김중완;최혜경;손윤희;임종국;이항우;남경수
  3. 생약학회지 v.30 시호 약침제재가 생쥐의 면역활성에 미치는 영향 문진영;임종국;최혜경;이임태;이항우;남경수
  4. 약학회지 v.42 별불가사리 렉틴의 복수암에 대한 항암효과 손윤희;전경희;최수정;정시련
  5. 申氏本草學 申佶求
  6. 中藥大辭典 安德均
  7. 東醫寶鑑 (pp. 90, 91, 92, 118, 218, 238, 232, 332, 373, 382, 385, 396, 424, 476, 477, 576, 589, 682, 720) 許 浚
  8. Cancer Chemother. Rep. Current status of clinical immunotherapy Bluming, A. Z.
  9. J. Tissue Culture Meth. v.9 A simple quantitative procedure using monolayer cultures for cytotoxicity assays (HTD/Nr-90) Borenfreund, E.;J. A. Puemer
  10. Fundamentals of cancer chemotherapy Cancer chemotherapy and its impact on the immune system Braun, D. P.;J. E. Harris;Hellmann, K(ed.);S. K. Harris(ed.)
  11. Cancer Res. v.47 Evaluation of a tetraxolium-based semautomated colorimetric assay: Assessment of chemosensitivity testing Carmichael J.;W. G. DeGrff;A. F. Gazdar;J. D. Minna;J. B. Mitchell
  12. New drug development from national production Assay of mutagenicity and antimutgenicity of traditional herbal drugs by using SOS-chromolumutest and micronucleus test in mice Chang, I. M.
  13. Cancer v.54 Clinical trials for the evaluation of cancer therapy Fish, B.
  14. Cancer Res. v.47 Effect of verapamilon in vitro cytotoxicin and vinblastine in human tumor cell Goodman, G. E.;Y. P. Yen;T. C. Cox;J. Crowlry
  15. J. Am. Anim. Hosp. Assoc. v.12 General concepts of immunotherapy of tumors Mac Ewen, E. G.
  16. Immunotherapy of human cancer Active immunotherapy : Experimental and rational basis Mathe, G.
  17. Gan To Kadaku Ryoho v.20 Pharmacodynamics and action mechanism of antitumor agents Nakamura, T.;T. Ueda
  18. J. Am. Vet. Med. Assoc. v.181 Comparative aspects of cancer immunotherapy Theilen, G. H.;D. Hills
  19. Cancer Res. v.51 Tetrazolium-based assays for cellular viability: A critical examination of selected parameters affecting formazan production Vistica, D. T.;P. Skehan;D. Scudiero;A. Monks;A. Pittman;M. Boyd
  20. Tumor antigenicity and approaches to tumor immunotherapy Weiss, D. V.