Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Isolation and Identification of Serratia sp. Producing Cephalosporin C Amidase

Cephalosporin C Amidase를 생산하는 Serratia sp. 균주의 분리와 동정

  • 신중철 (영남대학교 응용화학공학부) ;
  • 강용호 (영남대학교 응용미생물학과) ;
  • 김영수 (영남대학교 응용화학공학부)
  • Published : 1999.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Various side-chains are introduced to the 7-amino position of 7-aminocepha-losporanic acid (7-ACA) to make semi-synthetic cephalosporin antibiotics. In order to convert cephalosporin C (CPC) to 7-ACA, two enzymatic reactions are generally imployed. Glutary1-7-aminocephalosporanic acid (Gl-7-ACA) acylase is involved in the second step where the reaction intermediate, Gl-7-ACa is converted into 7-ACA. It was recently reported that CPC amidase can convert CPC directly into 7-ACA in a single enzymatic reaction. A study was undertaken to screen microorganisms conferring enzyme activity to convert Gl-7-ACA or CPC into 7-ACA by one or two enzymatic reactions. In order to screen the microorganisms rapidly, a non-$\beta$-lactam model compund, glutaryl-$\rho$-nitroanilide, was utilized in an early stage, thereafter the selected microorganisms were examined with real substrates. One microorganism exhibiting both Gl-7-ACA acylase and CPC amidase activities was obtained by the colorimetry method and HPLC assay, and was identified as a strain of Serratia species, designated as Serratia sp. N14.4. The optimal fermentation conditions for Serratia sp. N14.4 was pH9.0 and 3$0^{\circ}C$.

Keywords

References

  1. Biotechnol. Lett. v.19 Enzymatic conversion of cephalosporin C into glutaryl 7-aminocephalosporanic acid. A study in different reactor configurations Alfani, F.;M. Cantarella;N. Cutarella;A. Gallifuoco;P. Golini;D. Bianchi
  2. J. Bacteriol. v.173 Cloning of a novel 7 β-(4-Carboxybutanamido)-cephalosporanic acid acylase gene of Bacillus laterosporus and its expression in Escherichia coli and Bacillus subtilis Arami, I.;M. Fukagawa;M. Tsumura;M. Iwami;H. Ono;H. Kojo;M. Kohsaka;Y. Ueda;H. Imanaka
  3. J. Ferment. Bioeng. v.72 Cloning and nucleotide sequencing of new glutaryl 7-ACA and cephalosporin C acylase genes from Pseudomonas strains Arami, I.;M. Fukagawa;M. Tsumura;M. Iwami;T. Isogai;H. Ono;Y. Ishitani;H. Kojo;M. Kohsaka;Y. Ueda;H. Imanaka
  4. J. Ferment. Bioeng. v.72 Isolation of soil strains producting new cephalosporin C acylases Aramori, I.;M. Fukagawa;M. Tsumura;M. Iwami;Y. Yokota;H. Kojo;M. Kohsaka;Y. Ueda;H. Imanaka
  5. J. Ferment. Bioeng. v.73 Comparative characterization of new glutaryl 7-ACA and cephalosporin C acylases Aramori, I.;M. Fukagawa;M. Tsumura;M. Iwami;Y. Yokota;H. Kojo;M. Kohsaka;Y. Ueda;H. Imanaka
  6. Bergey's Manual of Systematic Bacteriology v.2 Genus Serratia sp. Claus, D.;R. C. W. Berkeley;P. H. A. Sneath(ed.)
  7. Biotechnol. Bioeng. v.46 Two step immobilized enzyme conversion of cephalosporin C to 7-aminocephalosporanic acid Conion, H. D.;J. Baqai;K. Baker;Y. Q. Shen;B. L. Wong;R. Noiles;C. W. Rausch
  8. Appl. Microbiol. Biotechnol. v.43 Screening characterization of microorganisms with glutaryl-7-ADCA acylase activity Franzosi, G.;E. Battiste;I. Gagliardi;W. Vander Goes
  9. Biotechnol. Lett. v.14 High yielding culture conditions for the biosynthesis of D-amino acid oxidase by Trigonopsis variabilis Huber, F. M.;J. T. Vicenzi;A. J. Tietz
  10. J. Ferment. Bioeng. v.77 no.6 A novel 7-β-(4-carboxybutanamido)-cephalosporanic acid acylase isolate from Pseudomonas strain C427 and its high-level production in Escherichia coli Ishi, Y.;Y. Saito;T. Fujimura;T. Isogai;H. Koji;M. Yamashita;M. Niwa;M. Kohsaka
  11. Eur. J. Biochem. v.230 High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176 Ishi, Y;Y. Saito;T. Fujimira;H. Saito;H. Sasaki;Y. Noguchi;H. Yamada;M. Niwa;K. Shimomura
  12. Biotechnol. Biopress Eng. v.2 Isolation and characterization of soil strains producing glutaryl-7-aminocephalosporanic acid acylase Khang, Y. H;B. H. Yoo
  13. J. Ferment. Bioeng. v.77 no.6 Chemical modification and site-directed mutagenesis of tyrosine residues in cephalosporin C acylase from Pseudomonas strain N176 Nobbs, T. J.;Y. Ishi;T. Fujimura;Y. Saito;M. Niwa
  14. Eur. J. Biochem. v.180 Properties of D-amino acid oxidase from Rhodotorula gracilis Pilone, M. S.;L. Pollegioni;P. Casalin;B. Curti;S. Ronchi
  15. Biotechnol. Techniq. v.1 Evaluation of determination of 6-aminopenicillanic acid by p-dimethy1 aminobenzaldehyde Shewale, J. G.;K. K. Kumar;G. R. Ambekar
  16. Process Biochemistry v.27 Molecular aspects of penicillin and cephalosporin acylases Sudhakaran, V. K.;B. S. Deshpande;S. S. Ambedkar;J. G. Shewale
  17. Industrial Application of Immobilized Biocatalysts Production of 6-APA, 7-ACA, and 7-ADCA by immobilized penicillin and cephalosporin amidase Tanaka, A.;T. Tosa;T. Kobayashi