Effect of Extracellular Polymeric Substances(EPS) on the Biosorption of Lead by Microorganisums

납의 생물흡착에 미치는 세포외고분자물질의 영향

  • 서정호 (울산과학대학 공업화학과) ;
  • 김동석 (대구효성가톨릭대학교 환경과학과) ;
  • 송승구 (부산대학교 화학공학과)
  • Published : 1999.02.01

Abstract

Comparison of lead removal characteristics between two strains, Aureobasidium pullulans and Saccharomyces cerevisiae, and effects of extracellular polymeric substances(EPS) excreted by microorganisms on the removal of lead were investigated. The capacity of lead biosorption to A. pullulans which had EPS was increased as the storage time of the cells increased, due to the increased amounts of excreted EPS. When the EPS were removed from A. pullulans cells, the amounts of adsorbed lead were very small(10% of the cell with EPS). In the case of s. cerevisiae which had no EPS, the lead removal capacity was nearly constant with storage time except early stage, but the spending time to reach an equilibrium state decreased with increasing storage time because of lowering the function of cell membrane. Therefore, it seems that the phenomena of lead biosorption were remarkably affected by the presence of extracellular polymeric substances.

A pullulans와 S Cerevisiae의 납 제거 특성을 비교하고, 미생물이 분비하는 세포외고분자물질의 영향에 대해 고찰하였다. A pullulans의 경우에 미생물의 보관시간이 증가할수록 미생물이 분비하는 세포외고분자물질의 양도 증가하였으며, 납 제거능도 우수해졌다. 그러나 세포외고분자물질을 제거한 A pullulans세포에서는 납 흡착량이 약 10%로 매우 적었다. S Cerevisiae의 경우에는 세포외고분자물질은 거의 분비되지 않았으며, 보관시간에 따른 납 흡착량의 변화는 거의 없었다. 또한 보관시간이 경과할수록 흡착 평형에 도달하는 시간은 점점 짧아졌다. 따라서 A pullulans와 S Cerevisiae의 납제거 기작은 세포외고분자물질의 유무에 따라 매우 달라짐을 알 수 있었다.

Keywords

References

  1. Handbook of Ecotoxicology Hoffman, D. J.;B.A. Rattner;G. A. Bruton Jr.;J. Cairns Jr
  2. Sept. EPA Recovery of Metals from Sludge and Wastewaters EPA/600/s2-91/041
  3. Biotechnol. Prog. v.11 Riosorption of heavy metals Volesky, B.;Z. R. Holan
  4. 대한황경공학회지 v.19 Sacharomyces cerevisia와 Aureobasidium pullulans의 납 흡착 서정호;오상진;박영식;김동석;송승구
  5. Biosorption of heavy metals, Boca Raton, Fla Voleskey, B.
  6. Enviromental Engineering Research v.2 The biosorption rate of lead by Aureobas-idium pullulans Suh, J. H.;D. S. Kim;S. J. Oh;Y. S. Park;S K. Song
  7. Biotechnology Letters v.20 Progress of $Pb^{2+}$ bioaccumulation in Saccharomyces cerevisiae Suh, J. H.;D. S. Kim;J. W. Yun;S. K. Song
  8. Applied Biochemistry and Biotechnology v.61 Solution and particle effects on the biosorption of heavy metals by seaweed biomass Leusch, A.;Z. R. Holan;B. Volesky
  9. Biosurfactants and Biotechnology Kosaric, M.;Cairns, W. L.;Gray, Neil C. C.
  10. Proc. (27th) Ind. Waste Conf., Purdue Univ. Eng. Ext. Ser. No. v.141 Removal of mineral ions from water by microbially produced polymers Dugan, P. R.;Pickrum. H. M.
  11. Microb. Ecol. v.4 Influence of extracel lular polysaccharides on the toxicity of copper and cad mium toward Klebsiella aerogenes Bitton, G.;Freihofer, V.
  12. Microbial Mineral Recovery Extracellular polymers for metal binding Geesey, G.;jang, L.
  13. Zn. and Ag. Mar. Ecol., Ser. v.22 Effects of adh-erent bacteria and bacterial extracellular polymers upon assimilation by Macoma balthica of sediment bound Cd Harvey, R. W.;S. N. Luoma
  14. Appl. Environ. Microbiol. v.53 Che-lation properties of extracellular polysaccharides from Chlorella spp. Kaplan, D.;D. Christiaen;A. Shoshana
  15. Appl. Environ. Microbiol. v.60 precipitation of metallic cations by the acidic exopolysaccharides from Bradyrhizobium jap-onicum and Bradyrhizobium(Chamaecytisus) strain BGA-1 Carzo, J.;M. Leon-Barrios;V. Hernando-Rico;A.M. Gutierrez-Mavarro
  16. Elsevier Progress in industrial microbiology volume(18) : microbal polysaccharides Bushell, M. E.