Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Determination of Vitamin $B_{12}$ in Foods Using Column-Switching Technique in $\mu$-HPLC

$\mu$-HPLC의 Column-Switching 기술을 이용한 식품중 비타민 $B_{12}$의 분석

  • 박성진 (경희대학교 식품가공학과) ;
  • 김혜경 (한서대학교 식품생물공학과) ;
  • 함태식 (한서대학교 식품생물공학과) ;
  • 김병용 (경희대학교 식품가공학과)
  • Published : 1999.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Semi HPLC using a column switching technique was used to determine the trace content of vitamin B12 in various foods. Total analytical time required less than 20 mins per sample and the recovery ratio was 99.9, 99.6, 100.1 and 99.8% for 1.0, 10.0, 100.0 and 1,000 g/kg, respectively. The content of vitamin B12 in various foods obtained using column switching method showed higher levels compared to labels in dried milk(0.5 g/100g) and in grain products(0.51~34.36 g/100g). Thus, this column switching method was more sensitive, effective and precise than the microbiological analysis currently used to determine the trace compounds like a vitamin B12.

Keywords

References

  1. J. Chromatogr. v.183 High performance liquid chromatographic assay for measuement of cefoxitin in serum Wheeler,L.A.;De Meo,M.;Kirby,B.D.;Jerauld,R.S.;Finegold,S.M.
  2. J. Chromatogr. v.228 Determination of cefsulodin, cephalexin, cefotaxime, desacetylcefotaxim, cefuroxime and cefroxadin in plasma and urine by high performance liquid chromatography Lecaillon,J.B.;Rouan,M.C.;Souppart,C.;Fevre,N.;Juge,F.
  3. J. Chromatogr. v.275 Systermatic approach to the determination of cephalosporins in biological fluids by reversed phase liquid chromatography Rouan,M.C.;Abadie,F.;Leclerc,A.;Juge,F.
  4. J. Chromatogr. v.223 Determination of cephalosporins in biological material by reversed phase liquid cloumn chromatography Brisson,A.M.;Fourtillan,J.B.
  5. J. Chromatogr v.426 Microbore liquid chromatographic determination of cadralazine and cephalexin in plasma with large volume injection Rouan,M.C.
  6. J. Chromatogr. v.427 High performance liquid chromatographic assay of cephalexin in serum and urine Emm,T.A.;Leslie,J.;Chai,M.;Lesko,L.J.;Perkal,M.B.
  7. J. Chromatogr. v.433 Shielded hydrophobic phase; a new concept for direct injection analysis of biological fluids by high performance liquid chromatography Gisch,D.J.;Hunter,B.T.;Feibush,B.
  8. J. Chromatogr. v.424 Quantative analysis of retinoids in biological fluids by high performance liquid chromaography using column switching. Ⅰ. Determination of isotretinoin and tretinoin and their 4-oxo metabolites in plasma Wyss,R.;Buchell,F.
  9. Chromatographia v.30 Simulatenous determination of cefoxitin, cefuroxine, cephalexin and cephaloridine in plasma using HPLC and a column-switching technique Lee,Y.J.;Lee,H.S.
  10. J. Chromatogr. v.707 Column-switching high performance liquid chromatographic assay for the determination of quercetin in human urine with ultravilot absorbance detection Nielse,S.E.;Dragsted,L.O.
  11. Chromatographia v.44 Simultaneous determination of parathion and metabolites in serum by HPLC column switching Lee,H.S.;KIm,K.;Kim,J.H.;Do,D.S.;Lee,S.K.
  12. J. Microcol. Sep. v.7 Low concentration drug analysis by semimicrocolumn liquid chromatography with a polymercoated mixed-function precolum Shirota,O.;Suzuki,A.;Kanda,T.;Ohtsu,Y.;Yamaguchi,M.
  13. Brnseki Kagaku v.46 Continuous measurement of retinoic acid isomers and retinol in a plasma by column-switching reversedphase HPLC Asami,S.;Takamura,M.;Kimura,Y.;Suzuki,T.;Mugishima,H.;Uchikura,K.
  14. 식품의 기준·규격중 개정 보건복지부
  15. ビタミソ學實驗法(Ⅱ) 近藤春樹;奧田邦雄
  16. J. Chromatogr. v.174 High-performance liquild chromatographic separation of cobalamins Frenkel,E.P.;Kitchens,R.L.;Prough,R.
  17. ビタミソB₁₂の定量法 v.54 ビタミソ 在藤ㅡ精
  18. Nippon Shokuhin Kogyo Gakkaishi v.36 Study on the microbiological assay for the vitamin B₁₂of UHT cow's milk and infant formula using Lactobacillus leichmannii Kamei,T.;Sato,J.;Sugimoto,Y.;Suzuki,N.;Noda,K.
  19. ビタミソと補酵素(下) 植木 厚
  20. The United States Pharmacopeia (20th ed.)