Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Purification and Characterization of the Inhibitory Principle aganist Pancreatic Cholesterol Esterase from Ephedra herba

마황으로부터 췌장 Cholesterol Esterase 저해물질 분리 및 규명

  • 김희숙 (경성대학교 식품공학과) ;
  • 조은정 (경성대학교 식품공학과) ;
  • 류병호 (경성대학교 식품공학과) ;
  • 송병권 (부산식품의약품안전청 시험분석실) ;
  • 이태훈 (포항공과대학교 생명과학과) ;
  • 서판길 (포항공과대학교 생명과학과) ;
  • 류성호 (포항공과대학교 생명과학과)
  • Published : 1999.08.01
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Abstract

Cholesterol esterase(pCEH, pancreas cholesterol ester hydrolase, E.C.3.1.1.13) which is secreted from pancreas has been known as an important lipase for cholesterol uptake. cholesteryl acyl esters from a diet must be hydrolyzed to free cholesterol and fatty acid by cholesterol esterase before the absorption in small intestine. For the development of inhibitory substances from natural source, we screened many extracts of oriental herbs for the inhibition of cholesterol esterase in vitro. The ethanol extract of Ephedra herba showed strong inhibitory activity. Solvent fractionation and silica gel column chromatography with the extract lead to the purification of the inhibitory principle in Ephedra herba. Crystallized inhibitor was identified as ( ) ephedrine by using UV, FT IR, 1H NMR, 13C NMR and GC/Mass. These results suggest that ( ) ephedrine can be used as a potential lead compound for the development of inhibitor for cholesterol uptake by cholesterol esterase inhibition.

Keywords

References

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