Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지

Measurement of Inhibitory Activities on 3-Hydorxy-3-Methylglutaryl CoA Reductase and Acyl-CoA:Cholesterol Acyltransferase by Various Plant Extracts in vitro

시험관법에의한 식물열수추출물의 3-Hydroxy-3-Methylglutaryl CoA Reductase 및 Acyl-CoA:Cholesterol Acyltransferase 활성 저해도 측정

  • 최명숙 (경북대학교 식품영양학과) ;
  • 이희자 (경북대학교 식품영양학과)
  • Published : 1999.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Hydroxy methylglutaryl CoA(HMG CoA) reductase and acyl CoA:cholesterol acyltransferase(ACAT) are two important enzymes that are associated with regulation of cholesterol metabolism. The inhibitors of HMG CoA reductase and ACAT are very effective in lowering serum cholesterol in most animal species. In present study, various plant extracts with hot water were used to examine the inhibitory activities against HMG CoA reductase and ACAT that are involved in cholesterol biosynthesis and cholesterol esterification in tissues, respectively. The extracts of Fagophyrum rotundatum, Rosa multiflora, Rosa rugosa and Alisma orientalis exhibited significant inhibitory activities against the ACAT, 29%, 24%, 19%, and 18%, respectively. However the extracts of Typha augustifolia, Polygonum cuspidatum, Crataegus pinnatifida, Polygonum multiflorum inhibited the HMG CoA reductase activity by 53%, 42%, 37%, and 33% respectively. Results suggest that these plant extracts might play important roles in the regulation of the cholesterol metabolism in vivo.

Keywords

References

  1. Atherosclerosis v.108 Drug and alternative therapies for hyperlipidemea Stein, E. A.
  2. J. Lipid Research v.24 Role of acyl-CoA:cholesterol acyltransferase in cholesterol absorption and its inhibition by 57-118 in the rabbit Heidner, J. G.;Pickens, C. E.;Kelly, L. A.
  3. J. Lipid Research v.34 In vivo evidence that the lipid-regulating activity of the ACAT inhibitor CI-976 in rats is due to inhibition of both intestinal and liver ACAT Krause, B. R.;Anderson, M.;Bisgaier, C. L.;Bacon, T.;Bousley, R.;Dehart, P.;Essenburg, A.;Hamelehle, K.;Homan, R.;Kieft, K.;McNally, W.;Stanfield, R.;R. S.
  4. J. Lipid Research v.22 Acyl-CoA:cholsterol acyltransferase in human small intestine: its activity and some properties of the enymatic reaction Helgerud, P.;Saarem, K.;Norum, K. R.
  5. J. Lipid Research v.26 Role of acyl-CoA:cholesterol acyltransferase in cellular cholesterol metabolism Suckling, K. E.;Stange, E. F.
  6. Atherosclerosis v.113 TMP-153, a novel ACAT inhibitor, inhibits cholesterol absorption and lowers plasma cholesterol in rats and hamsters Sugiyama, Y.;Ishikawa, E.;Odaka, H.;Miki, N.;Tawada, H.;Ikeda, H.
  7. Arterosclerosis and Thrombosis v.11 Comparison of CI-976, an antiatherosclerotic activity in iliac-femoral and thoratic aortic lesions Bocan, T. M. A.;Mueller, S. B.;Uhlendorf, P. D.;Newton, R. S.;Krause, B. R.
  8. Experimental and Molecular Pathology v.44 REgulation of acyl-CoA: cholesterol acyltransferase activity in normal and atherosclerotic rabbit aortas: Role of a cholesterol substrate pool Peter, J. G.;Kathy, A. R.;Marie, A. P. Candy, S. R.
  9. Atherosclerosis v.112 Acyl-Coenzyme A:cholesterol acyltransferase(ACAT)inhibitors modulate monocyte adhesion to aortic endothelial cells Uday, S.;Erida, F.;Roger, S. M.
  10. Ann. Intern. Med. v.107 Lovastatin in the treatment of heterozygous familial hypercholesterolemia Havel, R. J.;Hunninghake, D. B.;Illingworth, D. R.;Lees, R. S.;Stein, E. A.;Tobert, J. A.;Bacon, S. R.;Bolognese, J. A.;Fost, P. H.;Lamkin, G, E.
  11. Proc Ⅸth international symposium on drug effecting lipid metabolism Kajinnami, K.;Toshimura, A.;Watanabe, A.
  12. Atherosclerosis v.73 Effect of CS-514(pravastatin) on VLDL-triglyceride kinetics in rats Yoshino, G.;Kazumi, T.;Kasama, T.;Iwai, M.;Iwatani, I.;Matsuba, K.;Matsushitia, M.;Baba, S.
  13. Atherosclerosis v.118 Comparative effects of simvastatin and pravastatin on cholesterol synthesis in patients with primary hypercholesterolemia Feillet, C.;Farnier, M.;Monnier, L. H.;Percheron, C.;Colette, C.;Descomps, B.;De Paulet, A. C.
  14. Biochim. Biophys. Acta. v.50 CS-514, a competitive in hibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase: tissue-selective inhibition of sterol synthesis and hypolipidemic effect on various animal specieces Tsujita, Y.;Kuroda, M.;Shimada, Y.;Tanzawa, K.;Arai, M.;Kaneko, I.;Tanaka, M.;Masuda, H.;Tarumi, C.;Watanabe, T.;Fujii, S.
  15. J. Med. Chem. v.36 Inhibitors of ACAT Bruce, D.;Roth, C.;John, b.;Milton, L. H.;Ann, H.
  16. American J. Cardiology v.60 Hypolipidemic effects of HMG-CoA reductase inhibitros in patients with hypercholesterolemia Illingworth, D. R.;Bacon, S.
  17. Atherosclerosis v.119 Plasma mevalonic acid, an index of cholesterol synthesis in vivo, and responsiveness to HMG-CoA reductase inhibitors in familial hypercholesterolaemia Naoumova, R. P.;Marais, A. D.;Mountney, J.;Firth, J. C.;rendell, N. B.;Taylor, G. W.;Thompson, G.
  18. Atherosclerosis v.90 Effect of simvastatin on receptor mediated metabolism of low density lipoprotein in guinea pigs Matsunaga, A.;Sasaki, J.;Takada, Y.;Hidaka, K.;Arakawa, K.
  19. Lipids v.18 Inhibition of cholesterol and fatty acid biosynthesis in liver enzymes and chicken hepatocytes by polar fractions of garlic Qureshi, A. A.;Abuirmeil, N.;Din, Z. Z.;Elson, C.;Burger, W. C.
  20. Lipids v.20 Suppression of cholesterogenesis by plant constituents Qureshi, A. A.;Burger, W. C.;Peterson, D. M.;Elson, C.
  21. Atherosclerosis v.64 Effects of pure capsaicinoids(capsacin and kigydrocapsacin) on plasma lipid and lipoprotein concentrations of turkey poults Negulesco, J. A.;Noel, S. A.;Newman, H. A. I.;Nabar, E. C.;Bhat, H. B.;Witiak, D. T.
  22. J. Biol. Chem. v.261 The structure of an inhibitor of cholesterol biosynsis isolated from barley Qureshi, A. A.;Burger, W. C.;Peterson, D. M.;Elson, C.
  23. J. Korean Soc. Food Nutr. v.24 anti-hyperlipidemic effect of phragmites communis and its active principles Choi, J. S.;Lee, J. H.;Young, H. S.
  24. J. Korean Soc. Food Nutr. v.26 Effect of Quercus acutissima CARRUTHERS extracts on the lipid metabolism Sung, I. S.;Kim, M. J.;Choi, S. Y.
  25. J. Korean Soc. Food Nutr. Effect of pine pollen on serum and liver lipids in rats on a fed high fat diet Lee, Y. J.;Park, M. H.;Hwang, S. W.;Bae, M. J.;Han, J. P.
  26. Kor. J. Gerontol. v.1 Effect of dietary supplementation with pine leaf on lipid parameters in rats Kim, J. D.;Yoon, T. H.;Choe, M.;Im, K. J.;Ju, J. S.;Lee, S. Y.
  27. J. Korean Soc. Food Nutr. v.25 Effects of pine needle extracts on serum and liver lipid contents in rats fed high fat diet Kang, Y. H.;Park, Y. K.;Ha, T. Y.;Moon, K. D.
  28. J. Korean Soc. Food Nutr. v.23 the effect of plantaginis semen on serum and hepatic lipid metabolism in fed high and low fat kiets Cho, S. Y.;Kim, M. J.
  29. J. Korean Soc. Food Nutr. v.23 Effect of dietary buckwheat vegetables on lipid metabolism in rats Choi, T. S.;Sur, J. H.;Kim, C. H.;Kim, Y. M.;Han, S. S.;Lee, S. Y.
  30. J. Korean Soc. Food Nutr. v.21 Effect of acanthopanacis cortex boiling extract solutions on fat accumulation in the obese rats induced by high fat dietary Sung, T. S.;Son, G. M.;Bae, M. J.;Choi, C.
  31. J. Korean Soc. Food Nutr. v.26 Effect of Angelica keiskei on lipid metabolism in rats Park, J. R.;Park, s. K.;Cho, Y. S.;Chun, S. S.;Choi, S. H.;Park, J. C.
  32. Life Sci. v.49 Influence of extract of Rosa Rugosa roots on lipid levels in serum levels in serum and liver of rats Lee, Y. S.;Kim, J. D.;Lee, Y. H.;Rhee, H. I.;Choi, Y. S.
  33. J. Natural Products v.54 Antihyperlipidemic effect of flavonoids from Prunus davidiana Choi, J. S.;Yokoza, T.;Oura, H.
  34. Korean J. Nutrtion v.26 Effect of mustard leaf(Brassica juncea) on cholesterol metabolism in rats Jo, Y. S.;Park, J. R.;Park, S. K.;Chun, S. S.;chung, S. Y.;Ha, B. S.
  35. The pharmacology of Chinese herbs Antihypercholesterolemic herbs Huang, K. C.;Huang, K. C.(ed.)
  36. J. Lipid Research v.14 Simplified spectrophotometric assay for mecrosomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. Hulcher, F. H.;Oleson, W. H.
  37. Anal Biochem. v.72 A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  38. Experimental and Molecular Pathology v.44 Regulation of acyl-CoA:cholesterol acyltransferase activity in normal and atherosclerotic rabbit aortas:Role of a cholesterol substrate pool Gillies, P. J.;Rathgeb, K. A.;Perri, M. A.;robinson, C. S.
  39. Biochem. Biophys. Acta. v.370 Micro assay for 3-hydroxy-3-methylglutayl CoA reductase in rat liver and in L-cell fibroblasts Shapiro, D. J.;Nordstrom, J. L.;Mitschelen, J. J.;Rodwell, V. W.;Schimke, r. T.
  40. Drugs v.SUP 3 An overview of lipid-lowering drugs Illingworth, D. R.
  41. Lipid Research v.22 Influence of nicotinic acid on metabolism of cholesterol and triglycerides in man Grundy, S. M.;Mok, H. Y.;Zech, L.;Berman, M.
  42. J. Korean Soc. Food Sci. Nutr. v.26 Screeing and characterization of anticholesterogenic substances from edible plant extracts Park, J. R.;Park, J. C.;Choi, S. H.
  43. Korean J. Biotechnol. Bioeng. v.6 In vitro screening of 3-hydroxy-3-methyl-glutaryl conenzyme A reductase inhibitor from plant extract Lee, Y. H.;Shin, Y. M.;Lee, J. E.;Choi, Y. S.;Lee, S. Y.