Bacterial Productivity and Degradability of Organic Compounds in the Coastal Area of East Sea

동해 연안역의 박테리아 생산력과 유기물질의 분해능

  • 이기성 (배재대학교 생물학과) ;
  • 고동규 (한양대학교 생물학과) ;
  • 김근호 (배재대학교 생물학과) ;
  • 이영근 (한국원자력연구소 방사성동위원소 방사선응용연구팀) ;
  • 최청일 (한양대학교 지구해양학과) ;
  • 최영길 (한양대학교 생물학과)
  • Published : 1999.03.01

Abstract

Bacterial population, signal compounds of pollution, bacterial productivity, heterotrophic activity and the activities of extracellular enzymes were analyzed four times from July 1994 to April 1995 at Daejin, Galnam, Pohang, and Ulsan in the coastal area of Bast Sea in order to investigate the state of eutrophication. The population of heavy metal resistant bacteria in Pohang was the highest among all stations. The bacterial populations which could utilize C -P compounds (phosphonate) showed the highest at Pohang that was expected for the industrial and agricultural effluents to inpour. It could be useful as an index of expectation about the influx of industrial sewages and agricultural effluents. The activities of extracellular enzymes for the degradation of organic matter were the highest in the Ulsan Chuyongam, but low in Daejin. These results showed positive relationship with the population of heterotrophic bacteria and bacterial productivity The ratio of poly-P and DNA in Pohang was the highest, and that in Ulsan, Galnam, and Daejin consecutively, corresponding with the distribution of heterotrophic bacteria. In case of the heterotrophic activities, each of the stations gives difference of seasonal variation in heterotrophic activities. Bacterial productivity seemed to be dependent on seasons; the highest in summer and the lowest in fall and winter. These seasonal variations of bacterial productivity seems to be similar with those of heterotrophic bacteria. It is suggested that the state of eutrophication could be evaluated by the criteria of the population sizes and the productivities of microorganisms, the amount of poly-P, heterotrophic activity, the activities of extracellular enzymes, etc.

동해 연안역의 부영양화 상태 및 종속영양세균에 의한 유기물질 분해능을 조사하기 위해서 대진, 갈남, 포항, 울산의 4개 지역의 연안역을 대상으로 1994년 7월부터 1995년 4월까지 4회에 걸쳐 종속영양세균군집, 오염의 신호화합물, 세균의 생산력, 종속영양활성도 및 세포외 효소활성도 등을 분석하였다. 중금속내성균의 수는 포항에서 가장 높게 나타났으며, 각종 유기인을 이용하는 세균군집을 조사한 결과 유기인(C-P)화합물이 많이 포함될 것으로 예상되는 산업폐수, 가정하수 및 농업폐수 등이 많이 유입되는 정점에서 이들 유기인 화합물을 분해하고 산화 환원시키는 세균군집이 높게 나타났다. 이들 세균의 출현빈도는 산업폐수, 가정하수 그리고 농업용수의 유입예측지표로 활용될 수 있으리라 생각된다. 세포외 효소활성도는 울산 처용암에서 가장 높았으며 대진에서 가장 낮게 나타났다. 이러한 결과는 종속영양 세균수와 세균의 생산성 등과 정의 상관관계를 나타내었다. Poly-P와 DNA의 경우, 정점별로는 포항이 가장 높았고, 울산, 갈남, 대진 순으로 나타났다. 이는 종속영양세균의 분포양상과 매우 일치하는 것으로 조사되었다. 종속영양 활성도는 정점별, 계절별 변화가 매우 심하였다. 세균의 생산력은 하계에 가장 높고 추계와 동계에 낮았다가 춘계에 다시 높아지는 경향을 나타나 종속영양세균의 계절적 변화양상과 유사한 경향을 보였다. 결과적으로 세균의 군집, poly-P함량, 세균의 생산력, 종속영양활성도 및 세포외 효소활성도 등의 분석자료는 부영양화 상태 및 유기물질의 순환과정을 파악할 수 있는 지표로 이용될 수 있다.

Keywords

References

  1. Appl. Environ. Microbiol. v.47 Inorganic phosphate accumulation and cadmium detoxification in Klebsoella aetogenes NCTC 418 growing in continuous culture Aiking H;A Stijnman;C Garderen;H Heerikhuizen;J Riet
  2. Appl. Environ. Microbiol. v.49 Bacterial biovolumn and biomass estimation Bratbask G
  3. Final report sponsored by Ministry of Education(1994 Project) Bacterial productivity in the coastal atea of East Sea Choi CI;KS Lee;YK Choi
  4. Appl. Environ. Microbiol. v.43 Diversity dynamics of marine bacteria studies by immunofluorescent standing on membrane filter Dahle AB;M Laake
  5. Appl. Envrion. Microbiol. v.64 Enzymatic activity, bactrial distribution and organic composition in sediments of the Ross Sea(Antarctica) Fabiano M;R Danovro
  6. J. Appl. Bacteriol. v.53 A method for estimating viability of aquatic bacteria by silde culture Fry JC;T Zia
  7. Microbial Ecology of a Blackish Water Environment Heterotrophic activity Gocke K;G. Rheinheimer(Ed.)
  8. Appl. Environ. Microbiol. v.54 Microbial decomposition in aquatic envroments: Combined process of extracellular enzyme activity and substrate uptake Hoppe HG;SG Kim;K Gocke
  9. Appl. Environ. Microbiol. v.58 Assessment of ${[^3H]}$thymidine incorporation into DNA as a method to determine bacterial productivity in stream bed sediments Kaplan LA;TL Bott;JK Bielicki
  10. J. Appl. Microbiol. v.83 Comparison between the evaluation of bacterial regrowth capability in turbidimeter and biodegrable dissolved organic carbon bioreactor measurements in water Kott Y;F Ribas;J Frias;F Lucena
  11. Appl. Environ. Microbiol. v.44 Heterotrophic activity and biodegradation of labile and regractory compounds by groundwater and stream microbial population Ladd TI;RM Ventullo;PM Wallis;JW Costerton
  12. Kor. J. Limnol. v.3 Self-clarification and degradation of organic compounds by microbial community in Kum Rever area Lee KS;YS Park;GJ Han
  13. Kor. J. Environ. Biol. v.11 Distribution of antibiotics or heavy metal resistant bacteria, and frequency of multi-drug resistance in Kum River area Lee KS;DK Ko;WC Choi;TY Oh;YS Park;CI Choi;YK Choi
  14. Kor. J. Environ. Biol. v.12 β-Lactamase expression patterns of ampicillin resistant bacteria isolated from Kum River area Lee KS;DK Ko;WC Choi;YK Mun;YS Park;CI Choi;YK Choi
  15. Kor. J. Environ. Biol. v.13 Degradability of organic compound and signal index of pollution in Daechung Reservoir lake and Kum River area Lee KS;DK Ko;YS Park;CI Choi;YK Choi
  16. Kor. J. Limnol. v.28 Vertical variation upon degradability of organic compound and signal index of pollution in Daechung Reservoir lake Lee KS;DK Ko;YS Park;CI Choi;YK Choi
  17. Kor. J. Limnol. v.28 Distribution and bio-degradability of microbes degrading recalcitrant agricaltural chemicals Lee KS;TY Oh;YS Park;YS Kim;YC Kim;JY Lim;KH Min;CK Kim
  18. Kor. J. Environ. Biol. Distribution of nutritive salts and organic matters in the coastal area of East Sea Lee KS;GH Kim;S Huh;YK Lee;CI Choi
  19. Appl. Environ. Microbiol. v.52 Cadmium and mercury resistant Bacillus strains from a salt Marsh and Boston Harbor Mahler I;HS Levinson;Y Wang;HO Halvorson
  20. Arch. Hydrobiol. Beih. Ergeb. Limnol. v.31 Accurate conversion factors for calculating bacteria growth rates from thymidine incorporation into DNA: elusice or illusive? Moriarty DJW
  21. A manual of chemical and biological methods for seawater analysis Parson TR
  22. Biol. Fertil. Soils. v.17 Enzymic analysis of microbial pattern and process Sinsabaugh RL
  23. Method for dilution antimicrobial susceptibility test for bacteria that growth aetobically Thornsberry C;J Anhalt;AL Barry;JL Cotton;EH Gerlach;RN Jones;RC Moelling;RA Norton
  24. Soil Biol. Biochem. v.24 Micro-plate colourimetric assay for endo-acting cellulase, xylanase, chitinase, 1,3-β-glucanase and amylase extracted from forest soil horizons Wirth SJ;GA Wolf