BOD Determination by Heavy Metal Tolerant Candida sp. LSY2

Candida sp. LSY2 중금속 내성주를 이용한 BOD 분석

  • 김말남 (상명대학교 자연과학대학 생물학과) ;
  • 이선영 (상명대학교 자연과학대학 생물학과)
  • Published : 1999.12.01

Abstract

A biochemical oxygen demand (BOD) sensor loaded with Candida sp. was designed and constructed for the quick measurement of the concentrations of biologically assimilable organic substances dissolved in water. The sensitivity of the sensor was higher for glucose, acetic acid, aspartic acid and glutamic acid than that for lactose, sucrose and lactic acid. The sensitivities of BOD sensors loaded with $Zn^{2+}$ tolerant and intact strains were almost identical while the sensitivity of sensor loaded with Cd$^{2+}$ tolerant strain was considerably lower. The sensitivities of the sensors loaded with intact or $Zn^{2+}$ tolerant strains did not change with the concentration of $Cd^{2+}$ in the aqueous solution while the sensitivity of the sensor loaded with $Cd^{2+}$ tolerant strain decreased slightly. The sensitivities of the sensors loaded with intact strain, $Zn^{2+}$ or $Cd^{2+}$ tolerant strains were not affected by the concentration of $Zn^{2+}$ in the aqueous solution.n.

용존 유기물의 농도를 BOD로 신속히 측정하여 폐수 처리 공정에 feed back할 수 있는 Candida BOD sensor를 이용하여 BOD를 측정하였다. Glucose, acetic acid, aspartic acid 및 glutamic acid에 대한 sensitivity는 lactose, sucrose및 lactic acid의 sensitivity보다 더 높게 나타나서 오염물질의 종류에 따라 BOD sensor의 출력 신호가 다르게 제시되었다.$Zn^{2+}$내성주가 충전된 BOD sensor는 중금속 비내성주의 경우와 유사한 정도의 감응도를 나타내었으나 $Cd^{2+}$ 내성주를 사용하였을 때는 매우 낮은 sensitivity를 보였다. 수용액의 $Cd^{2+}$의 농도가 증가함에 따라 중금속 비내성주와 $Zn^{2+}$ 내성주의 sensor 의 sensitivity는 거의 일정하였으나 $Cd^{2+}$ 내성주의 BOD sensor의 경우에는 sensitivity가 오히려 감소하였다. 반면 수용액 중의 $Zn^{2+}$ 의 농도가 증가함에 따라서는 중금속 비내성주, $Zn^{2+}$ 내성주 및 $Cd^{2+}$ 내 성주 모두 거의 동일한 감응도를 나타내었다.

Keywords

References

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