Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
권호 표지

Immunization of Recombinant Membrane Protein in Theileria sergenti

Theileria sergenti 재조합 항원단백질의 면역원성

  • Jin-ho Park (College of Veterinary Medicine, Chonbuk National University) ;
  • Seung-ok Lee (College of Veterinary Medicine, Chonbuk National University) ;
  • Joon-seok Chae (School of Veterinary Medicine, UC Davis, USA) ;
  • Oh-deog Kwon (College of Veterinary Medicine, Chonbuk National University) ;
  • Joo-mook Lee (College of Veterinary Medicine, Chonbuk National University)
  • Published : 1999.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Theileriosis에 대한 효율적인 예방대책을 마련하기 위한 일환으로 발현된 T. sergenti 재조합 항원단백질의 면역원성을 조사하였다. 먼저, E. coli 단백질 발현 vector인 pQE 32 plasmid vector를 이용하여 발현된 T. sergenti의 재조합 막표면단백질(KTs-MP)을 4개월령의 유우 송아지에 접종하였다. 그리고 접종된 송아지의 혈액변화상과 T. sergenti에 대한 항체가의 변화상을 분석한 결과, 재조합단백질의 접종에 의하여 항체가가 상승되는 것을 알 수 있었다. 그러나 재조합단백질의 접종만으로는 T. sergenti의 감염을 완전하게 예방하지는 못하였다.

Keywords

References

  1. Res Vet Sci v.50 no.1 Activation of bovine peripheral blood macrophages in Theileria sergenti infected calves Asaoka,H.;Onuma,M.;Kawamoto,S.(et al.)
  2. Kisaengchunghak Chapchi v.30 no.2 Immunogenicity and protective efficacy of solubilized merozoite enriched Theileria sergenti immunogens.Ⅰ: Protection against homologous stabilate challenge Baek,B.K.;Choi,I.H.;Kim,B.S.(et al.)
  3. Korean J Vet Res v.36 no.3 Rapid detection of Theileria sergenti by the polymerase chain reaction in Korean cattle Chae,J.S.;Lee,J.M.;Kwon,O.D.(et al.)
  4. Vet Parasitol v.20 no.1-3 Application of chemi and bioluminescence in studies of immunological reactions against protozoa Diesing,L.;Ahmed,J.S.;Schein,E.
  5. Parasitol Res v.81 no.6 Serum from Theileria sergenti-infected cattle accelerates the clearance of bovine erythrocytes in SCID mice Hagiwara,K.;Tsuji,M.;Ishihara,C.(et al.)
  6. Infect Immun v.63 no.1 Immunization of cattle with recombinant Babesia bovis merozoite surface antigen-1 Hines,S.A.;Palmer,G.H.;Jasmer,D.P.(et al.)
  7. Vaccine v.16 no.13 Delivery of the Theileria parva p67 antigen to cattle using recombinant vaccinia virus: IL-2 enhances protection Honda,Y.;Waithaka,M.;Taracha,E.L.(et al.)
  8. Dev Biol Stand v.62 The use of tissue culture attenuated live vaccine for Theileria hirci Hooshmand-Rad,P.
  9. Mol Biochem Parasitol v.56 no.1 Analysis of the genes encoding immunodominant piroplasm surface proteins of T. sergenti and T. buffeli by nucleotide sequencing and polymerase chain reaction Kawazu,S.;Sugimoto,C.;Kamio,T.(et al.)
  10. J Biol Chem v.193 Protein measurement with the Folin phenol reagent Lowry,O.H.;Rosenbrough,N.J.;Farr,A.L.(et al.)
  11. Vet Parasitol v.41 no.1-2 Genomic analysis of Theileria sergenti stocks in Japan with DNA probes Matsuba,T.;Kawakami,Y.;Iwai,H.(et al.)
  12. Proc Natl Acad Sci USA v.89 no.2 A recombinant sporozoite surface antigen of Theileria parva induces protection in cattle Musoke,A.;Morzaria,S.;Nkonge,C.(et al.)
  13. Nippon Juigaku Zasshi v.49 no.3 Antigenic properties of Theileria sergenti in ELISA serodiagnosis Ohgitani,T.;Okabe,T.;Sasaki,N.
  14. Trop Anim Health Prod v.29 Control of Theileria sergenti infection by vaccination Onuma,M.;Kubota,S.;Kakuda,T.(et al.)
  15. Z Parasitenkd v.47 no.2 On the life cycle of Theileria annulata in the midgut and hemolymph of Hyalomma anatolicum excavatum Schein,E.
  16. Nippon Juigaku Zasshi v.52 no.6 Opsonization of merozoites with sera from Theileria sergenti-infected calves Shinkawa,S.;Onuma,M.;Matsuba,T.(et al.)
  17. Nippon Juigaku Zasshi v.51 no.2 Abnormality of osmotic fragility and morphological disorder of bovine erythrocytes infected with Theileria sergenti Yagi,Y.;Furuuchi,S.;Takahashi,H.(et al.)
  18. 牛: Theileria 病. 獸 住血徵生物病 南哲郞;南哲郞(編著); 永撤(編著)
  19. 농시보고 v.12 국내 소의 주혈원충과 그 혈액학치에 관한 조사 연구 전영
  20. 농시보고 v.11 한우에 있어서 우의 파이로프라스마원충의 분포조사 및 인공감염시험 한치우;김삼기
  21. 가축위생연구보고 v.14 우리나라 축우의 소위 소형 Piroplasma병에 관한 연구 Ⅱ. 소위 소형 Piroplasma를 매개하는 진드기에 관하여 한치우