Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Biochemical Analysis of Anagrapha falcifera NPV Attachment to Spodoptera frugiperda 21 Cells

  • PARK, JIN O (Department of Genetic Engineering, Kyung Hee University) ;
  • JAI MYUNG YANG (Department of Life Science, Sogang University) ;
  • IN SIK CHUNG (Institute of Genetic Engineering, Kyung Hee University)
  • Published : 1999.06.01
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Abstract

The binding characteristics of Anagrapha falcifera nuclear polyhedrosis virus (AtNPV) to Spodoptera frugiperda 21 (Sf21) cells were investigated. The cells displayed an affinity of 4.7×10/sup 10/M/sup -1/ with about 3,300 binding sites per cell. The biochemical nature of the AfNPV-binding sites on the cell surface was also partially identified. Our findings suggest that the binding-site moiety has a glycoprotein component, but that the direct involvement of oligosacccharides containing N-acetylglucosamine or sialic acid residues in binding is unlikely, and that AfNPV entry into Sf21 cells may be via receptor-mediated endocytosis.

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References

  1. J. Gen. Virol. v.77 Physical map of Anagrapha falcifera multinucleocapsid nuclear polyhedrois virus Chen, C. J.;D. J. Leisy;S. M. Thiem
  2. Biotechnol. Lett. v.15 Effect of Trichoplusia ni BTI-Tn 5B1-4 cell density on human secreted alkaline phosphatase produciton Chung, I. S.;M. L. Shuler
  3. Baculovirus Expression Vector David, R. O.;L. K. Miller;V. A. Luckow
  4. Methods Enzymol. v.138 Glycosylation inhibitors for N-linked glycoproteins Elbein, A. D.
  5. J. Virol. v.67 Saturable attachment sites for polyhedron-derived baculovirus on insect cells an evidence for entry via direct membrane fusion Horton, H. M.;J. P. Burand
  6. Environmental Entomol. v.20 A new broad host spectrum nuclear polyhedrosis virus isolated from a celery looper, Anagrapha falcifera(Kirby). (Lepidoptera: Noctuidae) Hostetter, D. L.;B. Puttler
  7. J. Virol. v.68 Identification and characterization of the cell surface 70-kilodalton sialoglycoprotein(s) as a candidate receptor for encephalomyocarditis virus on human nucleated cells Jin, Y. M.;I. U. Pardoe;A. T. H. Burness;T. I. Michalak
  8. J. Virol. v.62 Infectious rotavirus enters cells by direct cell membrane penetration, not by endocytosis Kaljot, K.T.;R. D. Shaw;D. H. Rubin;H. B. Greenberg
  9. Animal Cell Technology: Basic & Applied Aspects v.8 Biochemical analysis of BmNPV attachment to Bombyx mori cells Kim, T. Y.;J. H. Park;J. O. Park;H. C. Kang;I. S. Chung;Funatsu(et al.,ed.)
  10. Am. J. Hyg. v.27 A simple method for estimating fifty percent endpoint Reed, L.;H. Muench
  11. Virology v.148 Alternate pathway of entry of budded Autograph californica nuclear polyhedrosis virus: Fusion at the plasma membrane Volkman, L. E.;P. A. Goldsmith;R. T. Hess
  12. J. Gen. Virol. v.66 Baculovirus replication: Uptake of Trichoplusia ni nuclear polyhedrosis virus particles by insect cells Wang, X. ;D. C. Kelly
  13. Ph. D. Thesis, Cornell University Baculovirus-Insect Cell Interactions in Producing Heterologous Proteins: Attachment. Infection and Expression in Different Cell Llines Wickham, T. J.
  14. J. Gen. Virol. v.73 Equilibrium and kinetic analysis of Autographa californica nuclear polyhedrosis virus attachment to different insect cell lines Wickham, T. J.;M. L. Shuler;D. A. Hammer;R. R. Granados;H. A. Wood
  15. J. Virol. v.69 Binding-kinetics of ecotropic (moloney) murine leukemia retrovirus with NIH 3T3 cells Yu, H.;N. Soong;W. F. Anderson