Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
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Gender Determination of X and Y-Specific Alphoid Repeat Sequences by PCR

PCR에 의한 X,Y-Specific Alphoid Repeat Sequences의 분석

  • Choi, Dong-Ho (DNA Analysis Section, National Institute of Scientific Investigation) ;
  • Kang, Pil-Won (DNA Analysis Section, National Institute of Scientific Investigation) ;
  • Lee, Yang-Han (DNA Analysis Section, National Institute of Scientific Investigation) ;
  • Han, Myun-Soo (DNA Analysis Section, National Institute of Scientific Investigation)
  • 최동호 (국립과학수사연구소 유전자분석실) ;
  • 강필원 (국립과학수사연구소 유전자분석실) ;
  • 이양한 (국립과학수사연구소 유전자분석실) ;
  • 한면수 (국립과학수사연구소 유전자분석실)
  • Received : 1998.08.28
  • Published : 1999.02.25
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Abstract

Recently, it has been possible to the alphoid gene, which has X and Y specificity, and determine the sex from human physical evidence using PCR methods. Samples from single sources, PCR method applied to the alphoid gene results in highly sensitive and accurate results even when only 60 pg of the genomic DNA was available for sex determination. Even for samples containing DNA from more than one gender source where the female DNA was present in the amount 10 times than that of the male, sex determination was possible. Therefore, this result suggests that alphoid gene, which has X and Y specificity, could be used effectively for sex determination in case of mixed DNA samples from biological evidence.

사람에게서 유래된 DNA시료의 X,Y 특이의 alphoid gene을 PCR법으로 분석하면 성별을 확인할 수 가 있다. PCR법으로 alphoid gene을 분석한바 매우 예민도가 높아 genomic DNA 약 60pg까지 성별을 분석할 수 있었다. 그리고 성별이 혼합되어 있는 DNA에서 female DNA의 1/10비 까지는 male DNA를 분석할 수 있었다. 따라서 이 결과는 혼합된 DNA에서 X,Y 특이의 alphoid gene을 분석하는데 기준으로 활용할 수가 있다.

Keywords

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