Korean Journal of Veterinary Research (대한수의학회지)
- Volume 39 Issue 1
- /
- Pages.20-26
- /
- 1999
- /
- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
Immunohistochemical study of neurotensin-, pancreatic polypeptide- and gastrin/cholecystokinin-immunoreactive cells in the gastrointestinal tract of the bullfrog, Rana catesbeiana during developmental stages
개체발생에 따른 황소개구리(Rana catesbeiana) 위장관에서 neurotensin, pancreatic polypeptide 및 gastrin/cholecystokinin 면역반응세포에 대한 면역조직화학적 연구
- Lee, Hyeung-sik (Faculty of Basic Science, College of Natural Science, Kyungsan University) ;
- Ku, Sae-kwang (Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University) ;
- Lee, Jae-hyun (Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University)
- Received : 1998.11.30
- Published : 1999.03.22
Abstract
To investigate the regional distribution and relative frequency of the neurotensin-, pancreatic polypeptide(PP)- and gastrin/cholecystokinin(Gas/CCK)-immunoreactive cells in the gastrointestinal tract of the bullfrog(Rana catesbeiana) with developmental stages, group of bullfrogs subdivided into the tadpole with hindlegs, metamorphosed bullfrog with tail, 2 weeks after metamorphosed bullfrog and adult bullfrog, were stained by immunohistochemical methods (PAP methods). Neurotensin-immunoreactive cells were observed from the pylorus of the metamorphosed bullfrog with tail, but these cells were not detected after that periods. PP-immunoreactive cells were detected from the adult bullfrog in the pylorus, duodenum and ileum. These cells were most predominant in the pylorus. Gas/CCK-immunoreactive cells were observed from the adult bullfrog in the pylorus. According to these results, most of immunoreactive cells in the gastrointestinal tract of the bullfrog were appeared after the complete metamorphosed periods, in which the complete differentiation of structure of gastrointestinal tract were occurred, and variable changes of the regional distribution and relative frequency with developmental stages were observed.