Immunohistochemical study of neurotensin-, pancreatic polypeptide- and gastrin/cholecystokinin-immunoreactive cells in the gastrointestinal tract of the bullfrog, Rana catesbeiana during developmental stages

개체발생에 따른 황소개구리(Rana catesbeiana) 위장관에서 neurotensin, pancreatic polypeptide 및 gastrin/cholecystokinin 면역반응세포에 대한 면역조직화학적 연구

  • Lee, Hyeung-sik (Faculty of Basic Science, College of Natural Science, Kyungsan University) ;
  • Ku, Sae-kwang (Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University) ;
  • Lee, Jae-hyun (Laboratory of Histology, College of Veterinary Medicine, Kyungpook National University)
  • 이형식 (경산대학교 자연과대학 기초과학부) ;
  • 구세광 (경북대학교 수의과대학 조직학교실) ;
  • 이재현 (경북대학교 수의과대학 조직학교실)
  • Received : 1998.11.30
  • Published : 1999.03.22

Abstract

To investigate the regional distribution and relative frequency of the neurotensin-, pancreatic polypeptide(PP)- and gastrin/cholecystokinin(Gas/CCK)-immunoreactive cells in the gastrointestinal tract of the bullfrog(Rana catesbeiana) with developmental stages, group of bullfrogs subdivided into the tadpole with hindlegs, metamorphosed bullfrog with tail, 2 weeks after metamorphosed bullfrog and adult bullfrog, were stained by immunohistochemical methods (PAP methods). Neurotensin-immunoreactive cells were observed from the pylorus of the metamorphosed bullfrog with tail, but these cells were not detected after that periods. PP-immunoreactive cells were detected from the adult bullfrog in the pylorus, duodenum and ileum. These cells were most predominant in the pylorus. Gas/CCK-immunoreactive cells were observed from the adult bullfrog in the pylorus. According to these results, most of immunoreactive cells in the gastrointestinal tract of the bullfrog were appeared after the complete metamorphosed periods, in which the complete differentiation of structure of gastrointestinal tract were occurred, and variable changes of the regional distribution and relative frequency with developmental stages were observed.

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