Journal of Oral Medicine and Pain

Korean Academy of Orofacial Pain and Oral Medicine (대한안면통증구강내과학회)
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Culture of Rat Salivary Acinar Cells

백서 타액선 선포 세포의 배양

  • Lee, Sung-Woo (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University) ;
  • Han, Song (Dept. of Oral Biochemistry, College of Dentistry, Kangnung National University) ;
  • Kho, Hong-Seop (Dept. of Oral Medicine & Oral Diagnosis, College of Dentistry, Seoul National University)
  • 이승우 (서울대학교 치과대학 구강내과.진단학 교실) ;
  • 한송 (강릉대학교 치과대학 구강생화학 교실) ;
  • 고홍섭 (서울대학교 치과대학 구강내과.진단학 교실)
  • Published : 1999.06.30
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Abstract

We investigated the culture condition and effects of various growth factors on the culture of salivary gland acinar cells. Male, Sprague-Dawley rats (about 6 weeks old) were sacrificed and their submandibular, sublingual, and parotid glands were used as specimens. High oxygen level more than 90% and coating of Matrigel on culture dish were important factors to help increase the survival time of acinar cells, Proper concentration of enzymes such as collagenase and hyaluronidase during isolation steps was also important. Addition of various growth factors such as dexamethasone, insulin, transferrin, selenous acid, reduced glutathione, epidermal growth factor, isoproterenol, and putrescine in culture medium helped to increase lifetime of cultured salivary acinar cells.

Keywords

Acknowledgement

Supported by : 한국과학재단