Nutritional and Cultural characterizations of microorganism capable of producing antagonistic activity against Streptococcus mutans

S. mutans에 항균력(抗菌力)을 나타내는 균주(菌株)의 배양학적(培養學的) 성질(性質)

The optimum culture conditions for an antibiotics from Actinomyces sp. were investigated. The optimum composition of medium for antibiotics production was 1% glucose, 1% soybean meal, 0.5% NaCl, 0.1% $CaCO_2$, and the optimum initial pH was 7.0. And the antibiotics showed highest activity when the strain isolated from soil was aerobically cultivated at $28^{\circ}C$ for 72hours under the optimum conditions. A production of the antibiotics from Actinomyces sp. begins at the 36th hours and then reached the maximum at the stationary phase developed at the 72th hours under the optimum conditions.

토양시료로부터 치아우식원인균인 S. mutans에 대하여 항균력을 나타내는 방선균주를 순수분리하여 분리균주를 공시균주로 하여 항생물질 생성에 미치는 환경인자를 규명하였다. 본 공시균주의 항생물질 생성 최적배지조성은 Bacto-soytone 1%, glucose 1%, NaCl 0.5%, $CaCO_3$ 0.1% 였으며 배지의 초기 pH는 7.0이었다. 또한 항생물질은 $28^{\circ}C$에서 진탕배양시 생성의 최적조건이었으며 본 공시균주는 24시간 유도기를 거쳐 배양 72시간째 항생물질의 생성과 더불어 균의 최대증식도를 나타내었다.