Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Formation of Acycloretinoic Acid by Autoxidation of Lycopene

Lycopene의 자동산화에 의한 Acycloretinoic acid의 생성

  • Kim, Seon-Jae (Food Industrial Technology Research Center, Mokpo National University)
  • 김선재 (목포대학교 식품산업기술연구센터)
  • Published : 2000.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Acycloretinoic acid was prepared from acycloretinal by oxidation with Tollens reagent. Acycloretinoic acid was separated with Silica-HPLC and analyzed by ODS-HPLC with a photodiode array detector and by GC-MS. Lycopene was solubilized in toluene and aqueous Tween 40, and then oxidized by incubating at $37^{\circ}C$ under atmospheric oxygen. Acidic compound was produced by autoxidation of lycopene. Retention time, UV-Vis spectra and mass spectra of the acidic compound were identical to the standard acycloretinoic acid. Thus, acycloretinoic acid was confirmed to occur in vitro under oxidation condition of lycopene.

Acycloretinal을 Tollens 시약으로 처리하여 acycloretinoic acid를 얻었다. 생성된 표준품 acycloretinoic acid는 Silica-HPLC 및 ODS-HPLC를 통하여 정제를 행하고 GC-MS에 의해 동정하였다. Lycopene을 toluene과 Tween 40 수용액에서 $37^{\circ}C$ 조건으로 자동산화시킨 결과, acidic 화합물이 생성되었다. Lycopene의 자동산화로부터 생성된 acidic 화합물은 그 HPLC상의 거동, 분광학적 특성 및 mass spectra의 특성이 acycloretinoic acid 표준품과 잘 일치하였다. 이러한 결과로 in vitro상의 산화적 조건하에서 lycopene의 자동산화에 의해 acycloretinoic acid가 생성됨을 알 수 있었다.

Keywords