Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
권호 표지
DOI QR코드

DOI QR Code

Molecular Biological Study of Anti-cancer Effects of Bee Venom Aqua-acupuncture

봉독약침(蜂毒藥鍼)의 항암효과(抗癌效果)에 대한 분자생물학적(分子生物學的) 연구(硏究)

  • Park, Chan-Yol (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Seo, Jung-Chul (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Choi, Do-Young (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University) ;
  • Ahn, Byoung-Choul (Dept. of Acupuncture & Moxibustion Oriental Medical College, KyungHee University)
  • Published : 2000.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability and apoptosis were analyzed using MTT assay, tryphan blue assay, $[^3H]$thymidine release assay, flow cytometric analysis, and activity of caspase-3 protease activity assay. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, $[^3H]$thymidine release assay, and flow cytomet1 ric analysis of sub $G_1$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and Bcl-$X_L$ were down-regulated whereas Bax was up-regulated by bee venom treatment.

Keywords

References

  1. 蜂毒療法과 蜂鍼療法 金文昊
  2. 死亡原因 統計年報 v.16 19大分類에 의한 死因構造 統計廳
  3. 養蜂協會報 no.12¿? 崔大鳳
  4. 大韓韓醫學會誌 v.13 no.1 蜂毒鍼療法이 抗炎, 鎭痛 및 解熱에 미치는 效能에 관한 實驗的 硏究 高炯均
  5. 大韓鍼灸學會誌 v.10 no.1 蜂鍼毒療法이 抗痙攣에 미치는 影響 孔賢淑;高炯均;金昌煥
  6. 大韓韓方腫瘍學會誌 v.1 no.1 韓國韓醫學의 腫瘍硏究에 對한 現況(1980年 以後의 雜誌에 報告된 論文을 中心으로) 공현식;류봉하;박동원;류기원
  7. 大韓鍼灸學會誌 v.11 no.1 蜂鍼에 對한 考察 權奇祿;高炯均;金昌煥
  8. 大韓鍼灸學會誌 v.14 no.2 蜂毒藥鍼刺戟이 3-MCA 誘發 上皮腫에 對한 抗癌 및 免疫反應에 미치는 影響 權奇祿(外)
  9. 大韓韓醫學會誌 v.20 no.1 흰쥐에서 合谷穴 蜂毒藥鍼刺戟에 依한 開口反射의 反應 金利和;李栽東;盧植;閔炳一
  10. 大韓鍼灸學會誌 v.12 no.1 蜂毒療法이 흰쥐의 膝關節 炎症性 浮腫에 미치는 影響 都垣錫;張埈赫;金慶鎬;尹鍾和;金甲成
  11. 慶熙大學校 大學院 博士學位論文 産地別 蜂毒液藥鍼刺戟이 免疫機能低下에 미치는 影響 李京姬
  12. 大韓鍼灸學會誌 v.11 no.1 藥鍼用 蜂毒液의 安全性 評價에 關한 硏究 李宗錫(外)
  13. 東醫學會誌 v.2 no.1 3-MCA 誘發 上皮腫에 對한 海藻玉壺湯과 昆布의 抗腫瘍 效果와 免疫反應에 미치는 影響 이종률;채병윤
  14. 大韓鍼灸學會誌 v.15 no.1 봉독요법에 대한 한의학 최초의 문헌기록: 마왕퇴의서의 봉독요법 2예 인창식;고형균
  15. 大韓韓方腫瘍學會誌 v.1 no.1 韓醫學의 腫瘍에 對한 認識과 病理論 崔昇勳
  16. 實用 中醫辭典 朱文鋒
  17. Brit. Pharmacol. Histamine release by MCDP(401), a peptide from the venom of the honey bee Assem, E.S.;Atkinson, G.
  18. Chemistry and Pharmacology of Honey Bee Venom Rudolf, Barbara
  19. Venomous Animals and their Venoms v.3 Chemistry, pharmacology and toxicology of bee, wasp and hornet venoms Harbermann, E.
  20. Brit. Pharmscol. A peptide from the venom of the Honey Bee Assen, E.S.K.(et al.)
  21. J. Natl. Cancer Inst. v.86 frequent expressed transition mutations p53 in prostate Chi, S.G.;devere White, R.W.;Myers, F.J.;Siders, D.;Lee, F.;Gumerlock, P.H. https://doi.org/10.1093/jnci/86.12.926
  22. Anal. Biochem. v.162 Single-step method of RNA isolation by acid-guanidinium thiocyanate-phenol-chloroform extraction Chomczynski, P.;Sacchi, N.
  23. Cancer chemother Pharmacology v.17 no.3 Rapid chemosensitivity testing of human lung tumor cells using the MTT assay Cole, S.P.
  24. PCR Methods and Applications v.2 Quantitative or semi-quantitative PCR: reality versus myth Ferre, F. https://doi.org/10.1101/gr.2.1.1
  25. J. Immunother. v.12 no.1 Damage to mitochondrial respiration chain is related to phospholipase A2 activation caused by tumor necrosis factor Higuchi, M.(et al.) https://doi.org/10.1097/00002371-199207000-00005
  26. Jounal of Pediatric surgery v.30 no.6 Bcl-2 Oncoprotein Expression and apoptosis in neuroblastoma Ikeda, H.;Hirato, J.;Matsuyama, S.;Suzuki, N.;Takahashi, A.;Kuroiwa, M. https://doi.org/10.1016/0022-3468(95)90752-1
  27. Cell v.88 Programmed cell death in animal development Jacobson, M.D.;Weil, M.;Raff, M.C. https://doi.org/10.1016/S0092-8674(00)81873-5
  28. Can Res. v.57 Immunohistochemical Analysis of in Vivo Patterns of Expression of CPP32(Caspase-3), a Cell Death Protease Krajewska, M.;Wang, H.G.;Krajewski, S.;Zapata, J.M.;Shabaik, A.;Gascoyne, R.;Reed, J.C.
  29. Journal of immunologic methods v.65 no.1-2 Rapid colorimetric assay for cellular growth and survival : application to proliferation and cytotoxicity assays Mosmann, T. https://doi.org/10.1016/0022-1759(83)90303-4
  30. Boll. Soc. Ital. Biol. Sper. v.65 no.5 Cell adhesion in rat fibroblasts: effect of tumor promoters Pippia, P.;Sciola, L.;Meloni, M.A.;Barni, S.;Tilloca, G.
  31. Joun. of Chromatography v.408 Characterization of bee venom and its main components by high performance liquid chromatography Rader, K.;Wildfeuer, A.;Wintersberger, F.;Bossinger, P. https://doi.org/10.1016/S0021-9673(01)81820-3
  32. Neurobiology v.3 Apamin from bee venom Spoerri, P.E.
  33. Proc. Natl. Acad. Sci. USA v.93 Bax enhances paclitaxel-induced apoptosis through a p53-independent pathway Strobel, T.;Swanson, L.;Korsmeter, S.;Cannistra, S.A. https://doi.org/10.1073/pnas.93.24.14094
  34. Blood v.88 no.5 Upregulated Expression of Bcl-2 in multiple myeloma cells induced by exposure to Doxorubicin, Etoposide, and Hydrogen peroxide Tu, Y.;Xu, Feng-Hao;Jin Liu;Versco, R.;Berenson, J.;Fady, C.;Lichtanstein, A.
  35. Proc. Natl. Acad. Sci. USA v.86 no.24 Quantitation of mRNA by polymerase chain reaction Wang, A.M.W.;Doyle, M.V.;Mark, D.F. https://doi.org/10.1073/pnas.86.24.9717
  36. Br. J. Cancer v.67 Apoptosis Wyllie, A.H.