Abstract
Induction of phase II enzymes such as quinone reductase (QR) or glutathione S-transferase (GST) is considered a major mechanism of protection against initiation of carcingenesis. The induction of detoxification enzymes and glutathione were studied with Lonicerae Flos aqua-acupuncture solution (LFAS), Angelicae gigantis Radix aqua-acupuncture solution (AGRAS), and Gamdutang aqua-acupunture solution (GAS) in murine hepatoma cells grown in microtiter plate wells. LFAS, AGRAS and GAS were potent inducers of QR activity. LFAS was induced about 2.6-fold at concentration of $3{\times}$. AGRAS and GAS were also induced about 2.6-, 1.8-fold at concentration of $5{\times}$, respectively. In addition, GST activity was increased with LFAS, AGRAS, and GAS. GSH levels were increased about 2-fold with LFAS at concentration of $5{\times}$, 1.3-fold with AGRAS at concentration of $3{\times}$, and 1.2-fold with GAS at concentration of $5{\times}$. These results suggested that LFAS, AGRAS, and GAS may act as blocking agents against carcinogenesis by induction of phase II marker enzymes.
금은화, 당귀, 감두를 이용하여 조제한 각각의 약침액을 이용하여 암예방효과를 살펴 보았다. 발암물질을 무독화시키는 QR 생성 유도를 생쥐의 간암세포인 Hepa1c1c7과 측정하였으며, 그 결과 금은화 약침액, 당귀 약침액, 감두약침액을 처리한 Hepa1c1c7에서 QR생성이 유도되었으며 GSH 생성을 살펴본 결과 Hepa1c1c7 세포에서 실험에 이용된 모든 생약 약침액에 의한 GSH 생성이 증가하였고, GST 생성 또한 증가하였다.
