Korean Journal of Acupuncture

Society for Meridian and Acupoint (경락경혈학회)
권호 표지

The Effect of Juglandis Semen Extract Solution on Oxidant-Induced Alteration of Glutamate Uptake in Rabbit Brain Synaptosome

호도약침액(胡桃藥鍼液)이 가토(家兎) 뇌(腦)의 Synaptosome에서 Oxidant에 의한 물질이동계(物質移動系)의 장애(障碍)에 미치는 영향(影響)

  • Kim Tae-Kook (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Youn Hyoun-Min (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Jang Kyung-Jeon (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Song Choon-Ho (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University) ;
  • Ahn Chang-Beohm (Dept. of Acupuncture & Moxibustion, College of Oriental Medicine, Dongeui University)
  • 김태국 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동국대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동국대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동국대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동국대학교 한의과대학 침구경혈학교실)
  • Published : 2000.03.27
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to determine whether Juglandis semen extract solution (JLS solution) exerts protective effect against oxidant-induced inhibition of glutamate uptake by synaptosomes. Synaptosome was prepared from rabbit brain cortex. Glutamate uptake increased by incubation time during 10 minutes, which was significantly inhibited by 1mM t-buthylhydroperoxide(t-BHP). JLS solution prevented t-BHP-induced inhibition of glutamate uptake in a dose-dependent manner. t-BHP reduced glutamate uptake in dose-dependent fashion, which was significantly prevented by 2% JLS solution. t-BHP(1mM) and $ascorbate/Fe^{2+}(50/1{\mu}M)$ increased lipid peroxidation in synaptosomes by 5-fold, and it was significantly prevented by 2% JLS solution. $HgCl_2(0.1mM)$ inhibited glutamate uptake and increased lipid peroxidation. These changes were prevented by 2% JLS solution. Synaptosomal Na-K-ATPase activity was inhibited by t-BHP(1mM) and $H_2O_2(50mM)$, which was prevented by 2% JLS solution. The results indicate that JLS solution prevents oxidant-induced inhibition of glutamate by synaptosomes, and this may result from inhibition of lipid peroxidation induced by oxidants.

Keywords

References

  1. 精校黃帝內經素問 洪元植
  2. 醫鑑重磨 李圭晙
  3. 精校黃帝內經靈樞 洪元植
  4. N-Engl-J-Med. v.312 Oxygen-derived radicals in post-ischemic tissue injury McCord, J. M.
  5. Acta. Neurol. Scand. v.126 Oxidants and the central nervous system ; some fundamental questions. Is oxidant damage relevant to Parkinson's disease, Alzheimer's disease, traumatic injury or stroke? Halliwell, B.
  6. FASEB J. v.4 Role of oxygen free radicals in carcinogenesis and brain ischemia Floyd, R. A.
  7. J. Appl. physiol. v.71 Oxygen radical mechanisms of brain injury following ischemia and reperfusion Traystman, R. J.;Kirsch J. R.;Koehler R. C.
  8. Free Radic. Biol. Med. v.14 The relationship between excitotoxicity and oxidative stress in the central nervous system Brondy, S. C.;LeBel C. P.
  9. TINS v.16 A radical hypothesis for neurodegeneration Olanow, C. W.
  10. Ann. Rev. Pharmacol. Toxicol. v.21 Excitatory amino acid transmitters Wakins, J. C.;Evans R. H.
  11. Acta. Neurol. Scand. v.66 Selective neuron loss after cerebral ischemia of the rat ; possible role of transmitter glutamate Jorgensen, M. B.;Diemer N. H.
  12. Excitotoxins Excitotoxins ; an overview Olney, J. W.;Flux, K.;Roberts P.;Schwarz R.
  13. Trends Neurosci. v.8 Excitatory amino acids and anoxic-ischemic brain damage Meldrum, B.
  14. Ann New Yo가 Acad. Sci. v.738 Reactive oxygen species on neural transmission, The Neurobiology of NO. and OH. Pellar, T. C.;Golman S. C.;Keyser D. O.;Lee K. H.;Lepinski D. L.;Livengood D.;Myers L. S.;Chiuh, C. C.;Gillbert D. L.;Colton C. A.
  15. J. Neurosci. v.14 Glutamate uptake inhibition by oxygen free radicals in rat cortical astrocytes Volterra, A.;Trotti D.;Florid S.;Racagni G.
  16. 本草備要解析 楊東喜
  17. 本草綱目 李時珍
  18. 本草求眞 황궁수
  19. 東醫臨床方劑學 尹吉榮
  20. 대한약전의 한약(생약)규격집 지형준;이상인
  21. 本草學 李尙仁
  22. 大韓韓醫學會誌 v.17 no.1 胡桃藥鍼液의 抗酸化效果에 對한 硏究 金永海;金甲成
  23. 大韓鍼灸學會誌 v.15 no.1 胡桃水鍼이 家兎腎臟의 抗酸化酵素 活性化에 미치는 영향 姜亨定
  24. 大韓鍼灸學會誌 v.16 no.1 胡桃水鍼이 毒性物質에 의해 유발된 家兎의 急性腎不全에 미치는 영향 徐正浩
  25. 胡桃水鍼이 Glycerol에 의해 유발된 家兎의 急性腎不全에 미치는 영향 宋世勳
  26. 胡桃藥鍼液이 t-bytylhydroperoxide에 의한 肝損傷에 미치는 영향 박상원
  27. 胡桃藥鍼液이 家兎 腦組織의 $Na^+-pump 活性障碍에 미치는 영향 金東勳
  28. Brain Res. v.93 An improved method of the preparation of synaptosomal fraction in high purity Hajos, F.
  29. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  30. Lab. Invest. v.62 Biology of disease ; Mechanisms of cell injury by activated oxygen species Farber, J. L.;Kyle M. E.;Coleman J. B.
  31. Arch. Biochem. Biophys. v.271 Studies on ascorbate-dependent, iron-catalyzed lipid peroxidation Miller, D. M.;Aust S. D.
  32. Disease of the Kidney v.Ⅱ The cellular basis of nephrotoxicity Weinberg, J. M.;Schrier, R. W.;Gottschalk C. W.
  33. Kid. Int. v.50 Renal oxidant response induced by mercury Nath, K. A.;Croatt A. J.;Likely S.;Behrens T. W.;Warden D.
  34. Methods Enzymol. v.52 Microsomal lipid peroxidation Buege, J. A.;Aust S. D.
  35. J. Biol. Chem. v.66 The colorimetric determination of phosphorus Fiske, C. H.;SubbaRow Y.
  36. Free Radical Res. Commun. v.12-13 Therapeutic usages of oxygen radical scavengers in human disease ; myths and realities Greenwald, R. A.
  37. Ann. Rev. Physiol. v.52 Free radicals and their involvement during long-term myocardial ischemia and reperfusion Downey, J. M.
  38. 鍼灸學 全國韓醫大 鍼灸, 經穴學敎室
  39. 大韓鍼灸學會誌 v.12 no.1 藥鍼療法의 刺鍼部位別 吸收에 關한 硏究 千永實;李載東;崔道永
  40. 大韓鍼灸學會誌 v.7 no.1 水鍼療法에 關한 考察 崔旼燮;高炯均;金昌煥
  41. 原色臨床本草學 辛民交
  42. Merck Index Budavari, Susan
  43. Angew Chem. Int. Ed. v.25 Biochemistry of oxidant stress Sies, H.
  44. Br. J. Pharmacol. v.41 Role of transmitter uptake mechanisms in synaptic transmission Inversen, I. L.
  45. Nature v.234 Unique high affinity uptake systems for glycine, glutamate, and aspartic acids in central nervous tissue of the rat Logan, W. J.;Snyder S. H.