The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Sequence and Characterization of the Genomic Clone of the FVFD16 and FVFD30 Gene Isolated from Flammulina velutipes

팽이버섯에서 분리된 FVFD16과 FVFD30 유전자의 게놈클론의 염기서열 및 특성

  • 김둘이 (임업연구원 산림생물과) ;
  • 동지칙 (일본 북해도립 임산시험장)
  • Published : 2000.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

We isolated genomic clone of FVFD16 and FVFD30 gene specifically expressed during fruit body formation of Flammulina velutipes [(Curt: Fr.) Sing] and determinated the sequences. The FVFD16 gene is including two introns in open reading frame, and FVFD30 gene is including four introns. The introns were matched GT/AG rule. The FVFD16 and FVFD30 genes contained CAAT box with similarity arrange and TATA box. CT-rich region was presented before the transcription start point. FVFD30 gene is investigated that expected the most activity of CCACC arrange. The result of FVFD16 gene analysis showed 80% homology by cDNA clone that is gene family. From the results of genomic southern blot analysis, we presumed more than two copy number gene family of FVFD16 and FVFD30 gene.

팽이버섯의 자실체형성 과정에 특이적으로 발현하는 FVFD16과 FVFD30 유전자의 genomic 클론을 단리하여 염기서열을 분석하였다. FVFD16은 Open Reading Frame(ORF)내에 2개의 intron이 관찰되었고, FVFD30 유전자에서는 4개의 intron이 관찰되었다. 또한 intron의 특징적인 염기서열인 GT/AG의 rule과도 일치하고 있음이 밝혀졌다. FVFD16과 FVFD30의 두 유전자 모두에서 진핵생물의 promoter영역에서 자주 관찰되는 CAAT box와 유사한 배열과 TATA box가 존재했다. 또한, 전사개시점의 바로 앞에서 관찰되어지는 CT-rich의 영역이 존재하고 있었으며, 특히 FVFD30에서는 전사개시 시에 중요한 역할을 할 것으로 예상되는 CCACC의 서열이 관찰되었다. 한편 FVFD16 genomic클론의 염기서열 분석결과 cDNA클론과 80%의 상동성을 나타내는 gene family임이 밝혀졌다. 여러 가지 제한효소에 의한 genomic southern blot 분석결과 FVFD16과 FVFD30은 2번 이상의 반복배열 또는 gene family의 존재가 확인되었다.

Keywords

References

  1. RNAの 世界;サイエンテイフイク 大澤 省三;志村 令郞
  2. 遺傳子工學の 基礎 野島 博
  3. 擔子菌類. 徽生物遺傳子實驗法 武丸 恒雄
  4. Mokuzai gakkaishi v.42 no.7 Isolation of a gene specifically expressed during fruiting body differentiation in Flammulina velutipes Azuma, T.;Harada, A.;Kim, D.Y.;Sakuma, Y.;Kojima, Y.;Miura, K.
  5. Mokkuzai gakkaishi v.42 no.9 Sequence and characterization of the genomic clone of the FDS gene from the basidiomycete Flammulina velutipes Azuma, T.;Yao, I.;Kim, D.Y.;Sakuma, Y.;Kojima, Y.;Miura, K.
  6. EMBO J. v.3 no.9 Sequence analysis of a split gene involved in fruiting from the fungus Schizophyllum commune Dons, J.J.M.;Mulder, G.H.;Rouwendal, G.J.R.;Springer, J.;Bremer, W.;Wessels, J.G.H.
  7. Gene v.139 A novel cDNA, priBC, endoding a protein with a Zn(II)2Cys6 zinc cluster DNA-binding motif, derived from the basidiomycete Lentinus edodes Endo, H.;Kajiwara, S.;Tsunoka, O.;Shishido, K.
  8. Proc. Natl. Acad. Sci. USA v.83 The qa repressor gene of Neurospora crassa: Wild-type and mutant nucleotide sequences Huiet, L.;Giles, N.H.
  9. The structure and organization of nuclear genes of filamentous fungi;Gene Structure in Eukaryotic Microbes Gurr, S.J.;Unkles, S.E.;Kinghorn, J.R.;Kinghorn, J.R.(ed.)
  10. Exp. Mycol. v.6 Change in complex RNA during fruiting-body initiation in the fungus Schizophyllum commune Hoge, J.H.C.;Sprigera, J.;Wessels, J.G.H.
  11. Cell v.43 A quantitative analysis of the effects of 5' junction and TACTAAC box mutants and mutant combinations on yeast mRNA splicing Jacquier, A.;Rodriguez, J.R.;Rosbash, M.
  12. Gene v.114 Isolation and sequence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes kajiwara, S.;Yamaoka, K.;Hori, K.;Miyazawa, H.;Saito, T.;Kanno, T.;Shishido, K.
  13. The Kor. J. Mycol. v.27 no.3 Cloning of a Gene Specifically Expressed During Early Stage of Fruiting Body Formation in Flammulina velutipes Kim, D.Y.;Azuma, T.
  14. Mushroom Sci. and Biotech. v.7 no.3 Cloning and Sequence Analysis of a cDNA for the Gene FVFD30, Specifically Expressed During Fruiting Body Development in Flammulina velutipes Kim, D.Y.;Azuma, T.;Harada, A.;Sakuma, Y.;Ando, A.;Tarnai, Y.;Miura, K.
  15. Gene v.154 A fruiting body-specific cDNA, mfbAc, from the mushroom Lentinus edodes a high-molecularweight cell-adhesion protein containing an Arg- Gly-Asp motif Kondoh, O.;Muto, A.;Kajiwara, S.;Takagi, J.;Saito, Y.;Shishido, K.
  16. FEMS Microbiol. Lett. v.130 Characterization of the promoter region of a cell-adhesion protein gene derived from the basidiomycete Lentinus edodes Kondoh, O.;Shishido, K.
  17. Cell v.33 Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts Langford, C.J.;Gallwitz, D.
  18. Cell v.36 Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast Langford, C.J.;Klinz, F.J.;Donath, C.;Gallwitz, D.
  19. Nucl. Acids Res. v.20 no.22 A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues Moller, E.M.;Bahnweg, G.;Geiger, H.H.
  20. Cell v.46 Nucleotide sequence of the Trisphoaphate isomerase gene from Aspergillus nidulans: Implications for a differential loss of introns Mcknight, G.L.;O'Hara, P.J.;Parker, M.L.
  21. EMBO J. v.4 no.10 Isolation and structural oraganization of the Neurospora crassa copper metallothionein Munger, K.;Germann, U.A.;Lerch, K.
  22. Gene v.32 Molecular characterization of the qa-4 gene of Neuroscrassa Rutledge, B.J.
  23. Mycol. Res. v.97 no.5 Fruiting genes of Schizophyllum commune are transcriptionally regulated Schuren, F.H.J.;van der Lende, T.R.;Wessels, J.G.H.
  24. Gene v.90 Two genes specifically expressed in fruiting dikaryons of Schizophyllum commune: homologics with a gene not regulated by mating-type genes Schuren, F.G.H.;Wessels, J.G.H.
  25. Mycol. Res. v.96 no.8 Gene expression during fruiting Schizophyllum commune Wessels, J.G.H.
  26. Nucl. Acids Res. v.11 no.16 The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences Woudt, L.P.;Pastink, A.;Kempers, A.E.;Jansen, A.E.M.;Mager, W.H.;Planta, R.J.