The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Histological Observation on the Symbiotic Relationships Between Gastrodia elata and Rhizomorph of Armillaria mellea

뽕나무버섯균 균사속과 천마의 공생관계에 대한 조직학적 관찰

  • Kim, Yong-Kyoo (Korea Gingeng and Tobacco Research Institute) ;
  • Km, Myung-Kon (Department of Food Resources, Iksan National College) ;
  • Yoon, Sook (Department of Food Science &Technology, Chonbuk National University) ;
  • Hong, Jai-Sik (Department of Food Science &Technology, Chonbuk National University)
  • Published : 2000.04.30
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Abstract

The histological studies were conducted to investigate the symbiotic relationships between Gastrodia elata and Armillaria mellea by using light and electron microscopes. The fungus, A. mellea, penetrated into the cortex of G. elata, in which endomycorrhizal mycelia in the cortical cells appeared to be dissolved and digested, and seemed to be consequently used as nutritional sources for G. elata growth. Staining of infected tissues revealed that protein- and fat-like substances were localized in the cells. The nuclei of cells infected by the fungal mycelia were hypertrophied 1.5 to 2 times as those without the fungal infection.

천마와 뽕나무버섯균 균사속과의 공생관계 규명을 위한 광학 및 전자현미경을 이용한 조직학적 연구를 수행한 결과 천마에 의해서 유도된 뽕나무버섯균의 균사속이 천마와 접촉하면 뽕나무버섯균 균사속의 침입에 의해 천마의 표피층은 심한 손상을 받게되며, 뽕나무버섯균의 균사속이 cortex층에 침입하여 내생균근을 형성하였다. 천마의 cortex층에 존재하는 내생균근으로부터 균사들이 각 피층세포에 침투해 들어간 균사속은 시간이 경과할 수록 균사결속이 상당히 와해되어지며, 균사속으로 부터 피층세포에 세포벽을 뚫고 세포 안으로 들어온 균사들은 천마에 의해 용해 분해 소화되어 뽕나무버섯 균사는 결국 천마의 영양원이 됨을 확인하였다. 또한 뽕나무버섯균의 침식을 받은 cortical cell에서 세포 및 핵이 비감염 부위보다 $1.5{\sim}2$배 가량 커짐을 확인 할 수 있다.

Keywords

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