Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

A Study on the Effects of Bee Venom Aqua-Acupuncture on Pain related Neuronal activity in the Spinal Cord

봉독약침(蜂毒藥鍼)이 척수내(脊髓內) 통증관련(痛症關聯) 신경세포(神經細胞)의 활성(活性)에 미치는 영향(影響)

  • Jeong, Sun-Hee (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Lee, Jae-Dong (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Koh, Hyung-Kyun (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Ahn, Byoung-Choul (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Choi, Do-Young (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Park, Dong-Suk (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University)
  • 정선희 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 고형균 (경희대학교 한의과대학 침구학교실) ;
  • 안병철 (경희대학교 한의과대학 침구학교실) ;
  • 최도영 (경희대학교 한의과대학 침구학교실) ;
  • 박동석 (경희대학교 한의과대학 침구학교실)
  • Published : 2000.06.20
Download PDF
⟨ Previous Next ⟩

Abstract

Introduction : In spite of the use of Bee Venom aqua-acupuncture in the clinics, the scientific evaluation on effects is not enough. Bee Venom aqua-acupuncture is used according to the stimulation of acupuncture point and the chemical effects of Bee Venom. The aims of this study is to investigate the analgegic effects of the Bee Venom aqua-acupuncture, through the change of writhing reflex and the change of c-fos in secondary neurons in the spinal cord. Materials and Methods : Pain animal model was used acetic acid method. The changes of writhing reflex of the mice which were derived pain by injecting acetic acid into the abdomen, after stimulating Bee Venom aqua-acupuncture on Chungwan(CV12) were measured. We used Fos immunohistochemical technique to study the neuronal activity in the spinal cord. Results : 1. Expression of c-fos in superficial dorsal horn(SDH), nucleus proprius(NP) and neck of dorsal hom(N) on 6~9th thoracic spine decreased significantly at $2.5{\times}10-4$g/kg Bee Venom aqua-acupuncture, compared with saline-acetic acid group. 2. The numeral change of Fos-LI neurons on the NP, N, and ventral gray(V) on 6-9th thoracic spine, SDH on 9-11th thoracic spine, and SDH and V on 11~13th thoracic spine decreased significantly at Chungwan(CV12) Bee Venom aqua-acupuncture, compared with saline-acetic acid group. 3. The correlation between the numbers of writhing refleax and Fos-LI neurons in T6-13 segment was statistically statistically significant at Chungwan(CV12) Bee Venom aqua-acupuncture. Conclusion : This study shows that the Bee Venom aqua-acupuncture on Chungwan(CV12) decreases the numbers of Fos-LI neurons. As the analgegic effects of Bee Venom aqua-acupuncture is recognized. Bee Venom aqua-acupuncture treatment is expected for pain modulation. In order to use it in many ways, more researches are needed for the dose and stability of Bee Venom aqua-acupuncture.

Keywords

References

  1. 생리학 강두희
  2. 痛症의 治療 고려의학출판부(역)
  3. 人體生理學 김광진
  4. 蜂毒療法과 蜂鍼療法 金文昊
  5. 奇蹟의 藥鍼療法 金廷彦
  6. 生理學 金正鎭
  7. 東洋醫學의 科學的 接近과 臨床 閔炳一
  8. 신경과학 대우학술 총서 자연과학 박찬웅;김승업
  9. 난치병의 蜂鍼療法 성은찬
  10. 알기쉬운 蜂鍼療法 성은찬
  11. 鍼灸學 全國韓醫學大學校鍼灸經穴學校室
  12. 양봉 새기술 최승윤
  13. 精解鍼灸學 崔容泰(外)
  14. 精校黃帝內經 洪元植
  15. 慶熙韓醫大論文集 v.5 大戟水鍼이 鎭痛 및 水銀 中毒에 미치는 影響에 關한 硏究 姜成吉
  16. 大韓韓醫學會誌 v.13 no.1 蜂鍼毒療法이 抗炎,鎭痛 및 解熱에 미치는 效果에 관한 實驗的 硏究 高炯均
  17. 大韓韓醫學會誌 v.12 no.1 蜂毒療法이 생쥐의 免疫反應에 미치는 實驗的 硏究 孔賢淑
  18. 大韓鍼灸學會誌 v.11 no.1 蜂毒에 對한 考察 權奇祿(外)
  19. 大韓鍼灸學會誌 v.15 no.2 蜂毒藥鍼療法이 消炎, 鎭痛作用에 미치는 效能에 관한 實驗的 硏究 權奇祿(外)
  20. 大韓鍼灸學會誌 v.4 no.1 鍼灸 및 Laser光線鍼刺戟이 白鼠의 鎭痛에 미치는 影響 金敬東;金甲成(外)
  21. 圓光大學校 大學院 學位論文集 v.10 艾灸가 膝關節炎의 消炎 및 鎭痛에 미치는 影響 金文甲
  22. 慶熙韓醫大論文 繆刺 및 巨刺法에 의한 電鍼刺戟이 消炎,鎭痛 및 血淸 cholinesterase活性에 미치는 影響 金文芳
  23. 大韓鍼灸學會誌 v.15 no.1 蜂毒藥鍼療法의 抗炎症 作用에 關한 實驗的 硏究 金祉榮;高炯均;金容奭(外)
  24. 慶熙韓醫大論文集 v.21 no.1 흰쥐의 鎭痛機轉에 있어서 視床下部 弓狀核의 役割에 對한 硏究 盧植;崔容泰
  25. 대한내과학회지 v.57 no.4 통증과 통증제어 기전 민병일
  26. 慶熙韓醫大論文 v.15 中脘 및 足三里의 蜂毒療法이 鎭痛作用에 미치는 影響 李宗錫;高炯均
  27. 大韓鍼灸學會誌 v.15 no.1 蜂毒療法에 大韓 韓醫學 最初의 文獻:馬王推醫書의 蜂毒療法제2例 인창식;고형균
  28. 血位注射療法 郭同經;
  29. 臨證指南醫案 v.5 葉天士
  30. 東垣十種醫書 李東垣
  31. 景岳全書 張景岳
  32. 儒門事親 v.2 張子和
  33. 實用中醫辭典 朱文鋒
  34. 陳無擇三因方 v.13 陳言
  35. 中西醫結合雜誌 v.4 蜂毒,蜂peptide抗炎,變應原性 및 急性毒性의 比較 陳推辛(外)
  36. Chemistry and Pharmacology of Honey Bee Venom Babara;Rudolf
  37. Text book of medical physiology Guyton
  38. The rat brain in stereotaxic cordinates LPaxinos G;Watson C
  39. Venom of the Hymenoptera Tom piek
  40. Pathology v.27 no.2 A high efficiency method for purification and assay od bee venom phosphlipase A2 Annand RR
  41. Nature v.245 An anti-inflammantory peptide from bee venom Billingham ME
  42. J.Comp. Neurol v.296 Expression of c-fos-like protein as a maker for neuronal activity following noxious stimulation in the rat Bullitt E
  43. Brain Res v.806 Spatial and temporal expression of c-Fos protein in the spinal cord of anesthetized rat induced by subcutaenous bee venom injection Ceng L;Jun C;Hui L.L.;Ji-Shuo L.
  44. Soc. Neurosci. Abst. v.17 Moving visual stimuli induce c-fos expression in the visual cortices of the rat. Craner SL;Lund JS;Hoffman GE;Lund RD
  45. J.Comp.Neurol. v.315 Morphine or U-5,488 supresses Fos protein-like immunoreactivity in the spinal cord and nucleus tractus solitarii evoked by a noxious visceral stimulus in the rat D.L. Hammond;Presley,R.;Gogas.K.R.;Basbaum,A.I.
  46. J.Allergy clin Immunol. v.95 no.2 IgE+ cells in the peripheral blood of atopic, nonatopic, and bee venom-hypersensitive individuals exhibitthe phenotype of highly differented B cells Donohoe PJ(et al)
  47. J.Immuol. v.155 no.5 A link between catalytic activity,Ige-independent mast cell activation,and allergencicity of bee venom phospholipase A2 Dudler T(et al)
  48. J.Pathol. v.177 no.1 An infarct-like myocardial lesion experimentally induced in Wister rats with Africanizied bee venom Ferrira DB(et al)
  49. Sci. Sin. v.22 The role of central 5-hydroxytryptamine in acupuncture analgesia Han JS;Chou Ph;Lu ZC;Yang Th;Lu LH;Ren MF
  50. 52European J.Pharmacol. v.351 Mentamizol potintiiates Morphine efeects on Viseral pain and evoked c-Fos immunoreactivity in spinal cord Julian Tayor(et al)
  51. Br. J.pharmacol. v.50 Anti-inflammantory property of 401(MCD-peptide), a peptide from the venom of the bee Apis mellifera Hanson JM
  52. Brain Res. v.562 Activation of a specific vestbulo-olivary pathway following centripetal accerlation in the rat. Kaufman GD;Anderson JH;Beityz AJ
  53. The NMDA antagonist MK 801 reduces noxious stimulus-evoked FOS expression in the spinal cord dorsal horn In Kehl LJ;Gogas KR;Lichtblau L;Pollock CH;Mayer M;Basbaum AI;Wilcox GL
  54. Pain v.40 Graded chronic pain status, an epidermiologic evaluation K.M. Von;S.Dworrkin;R.L.Le
  55. Brain Res. v.52 Principal involvement of cycloxygenase-1-derived prostaglandins in the c-fos expression of the rat hind brain following visceral stimulation with acetic acid. Kusuhara,H;Fukunari,A.;Matsuyuki,H.;Okumoto,T
  56. Pain v.66 no.2 The bee venom test:a new tonic - pain test Lariviere WR
  57. NeuroReport v.3 Nitricoxide mediates Fos expression in the spinal cord induced by mechanical noxious stimulation Lee J.H.;Beitz,A.J.
  58. Brain Res. v.577 Electroacupuncture modifies the expression of c-fos in the spinal cord induced by noxious stimulation. Lee J.H.;Beitz,A.J.
  59. Pain v.52 Distribution fo brain stem and spinal cordnuclei associated with different frequencies of electroacupunture analgesia Lee j.H.;Beitz,A.J.
  60. J.Neuroscience Methods v.29 The use of c-fos as a metabolic marker in neuronal pathway tracing M.Dragunow;R.Faull
  61. Nature v.322 Role of ion flux in the control f c-fos expression Morgan JI;Curran T
  62. Neuron v.6 Molecular pathways of pain:Fos/Jun-mediated activation of a non canonical AP-1 site in the prodynorphin gene Naranjo JR;Bellstrom B;Achaval M;Sassone-Corsi P
  63. Am.J.chin.Med. v.3 On acupuncture analgessia and the Mechanism of pain S.A. Anderson;E.Holmgeren
  64. Biochem.Biophys. Res.Comm. v.238 Melittin binds to secretory phospholipase A2 and inhibits its enzynmatic activity. Shamsher SS;Peterson JW;Chopra AK
  65. Neuron v.4 The regulation and function of c-fos and other immediate early genes in the nervous system Sheng M;GreenBerg ME
  66. Oftal. zh v.20 Eyeball injury caused by bee sing Tsvetkov Ⅵ
  67. J.Comp. Neurol. v.245 Localization of cytochrome oxidase in the mammaliian spinal cord and dorsal root gangliqa with quantative analysis of ventral horn cells in monkeys Wong-Reley M.T.T.;G.H.Kageyama
  68. Soc. Neurosci. Abst. v.17 Neuroal mechanisms of acoustic startle reflex:anatomical analysis using c-fos. Wu MF;Fahringer HM;Kilduff TS;Siegel JM