Purification and Characterization of Chitinase from a Marine Bacterium, Vibrio sp. 98CJ11027

  • 박신혜 (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • 이정현 (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • 이홍금
  • Published : 2002.12.01

Abstract

Chitin-degrading marine bacterial strain 98CJ11027 was isolated from bryozoa from the coastal area of Cheju Island, Korea, and identified as a member of the genus Vibrio. The molecular mass of the main extracellular chitinase (chitinase I), purified from strain 98CJ11027, was estimated to be 98 kDa. The optimal condition for chitinase I activity is pH 6.0 and 45℃. The activity was inhibited by $Fe^+2$ and$Cu^+2$. Chitinase I displayed the hydrolysis type of chitobiosidase and catalyzed reversed hydrolysis leading to the synthesis of tetraacetylchitotetraose.

Keywords

References

  1. Nucleic Acids Research v.25 no.17 Gapped BLAST and PSI-BLAST:a new generation of protein database search programs Altschul,S.F.;T.L.Madden;A.A.Schaffer;J.Zhang;Z.Zhang;W.Miller;D.Lipman https://doi.org/10.1093/nar/25.17.3389
  2. Vibrio v.1 Bergey's Manual of Systematic Bacteriology Baumann,P.;A.L.Furniss;J.V.Lee;N.R.Krieg(ed.);J.G.Holt(ed.)
  3. Planta v.157 no.1 Chitinase in bean leaves:inductiom by ethylene,purification,properties,and possible function Boller,T.;A.Gehri;F.Mauch;U.Vogeli https://doi.org/10.1007/BF00394536
  4. Analytical Biochemistry v.72 no.1-2 A rapid and sensitive method for the quantitaion of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  5. Appl. Environ. Microbiol. v.61 Chitinolytic Enterobacter agglomerans antagonistic to fungal plant pathogens Chernin,L.;Z.Ismailov;S.Haran;I.Chet
  6. J. Bacteriol. v.180 Chitinolytic activity in Chromobacterium violaceum:substrate analysis and regulation by quorum sensing Chenin,L.;M.K.Winson;J.M.Thomson;S.Haran;B.M.Bycroft;I.Chet;P.Williams;G.S.A.B.Stewart
  7. PHYLIP(phylogenecy inference package) Felsenstein,J.
  8. Mycological Research v.99 no.4 New components of the chitinolytic system of Trichoderma harzianum Haran,S.;H.Schikler;I.Chet https://doi.org/10.1016/S0953-7562(09)80642-4
  9. Bioscience Biotechnology and Biochemistry v.59 no.10 Identification of the positions of disulfide bonds of chitinase from a marine bacterium Hayashi,K.;S.I.Sato;R.Takano;J.Tsujibo;H.Orikoshi;C.Imada;Y.Okami;K.Inamori;S.Hara https://doi.org/10.1271/bbb.59.1981
  10. Bioscience Biotechnology and Biochemistry v.61 no.1 Isolation and characterization of chitinase from a flake-chitin degrading marine bacterium.Aeromonas hydrophila H-2330 Hiraga,K.;L.Shou;M.Kitazawa;S.Takahashi;M.Shimada;R.Sato;K.Oda https://doi.org/10.1271/bbb.61.174
  11. Biochemical Systematics and Ecology v.19 no.5 Chitin biodegradation in sand dune s Hirano,S.;Y.Koishibara;S.Kitaura;T.Taneko;H.Tsushida;K.Murae;T.Yamamoto https://doi.org/10.1016/0305-1978(91)90054-4
  12. FEMS Microbiology Letters v.157 no.1 Production purification of chitinase from Ewingella americana,a recently described pathogen of the mushroom Inglis,P.W.;J.F.Peberdy https://doi.org/10.1111/j.1574-6968.1997.tb12772.x
  13. Russ. J. Mar. Biol. v.18 Occurrence of chitin-decomposing enzymes in marine and freshwater microorganisms Ivanova,E,P.;I.Y.Bakunina;N.M.Gorshkova;L.A.Romanenko;V.V.Mikhailov;L.A.Elyakova;V.V.Parfenova
  14. Journal of Biological Chemistry v.271 no.52 The chitin catabolic cascade in the marine bacterium Vibrio furnissii:Molecular cloning,isolation,and characterization of a periplastic chitodextrinase Keyhani,N.O.;S.Roseman https://doi.org/10.1074/jbc.271.52.33414
  15. FEMS Microbiology Letters v.90 no.2 Evaluation of the antifungal activity of the purified chitinase I from the filamentous fungus Aphanocladium album Kunz,C.;A.Ludwig;Y.Bertheau;T.Boller https://doi.org/10.1111/j.1574-6968.1992.tb05135.x
  16. Nature v.277 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli,U.K
  17. J. Microbiol. v.37 Bacterial diversity of cultureable isolates from seawater and a marine coral, Plexauridae sp. Lee,J.H.;H.H.Shin;D.S.Lee;K.K.Kwon;S.J.Kim;H.K.Lee
  18. Thin layer chromatography Sugars and derivatives Lewis,B.A.;F.Smith;E.Stahl(ed.)
  19. Nucleic Acids Research v.27 no.1 A new version of the RDP(Ribosomal Database Project) Maidak,B.L.;J.R.Cole;C.T.Parker;G.M.Garrity,Jr.;N.Larsen.;B.Li;T.G.Lilburn;M.J.McCaughey;G.J.Olsen;R.Overbeek;S.Pramanik;T.M.Schmidt;J.M.Tiedje;C.R.Woese https://doi.org/10.1093/nar/27.1.171
  20. Canadian Journal of Microbiology v.44 no.7 Purification,characterization,and antifungal activity of chitinase from Fusarium chlamydosporum,a mycoparasite to groundnutrust Mathivanan,N.;V.Kabilan;K.Murugesan https://doi.org/10.1139/cjm-44-7-646
  21. Analytical Biochemistry v.189 no.1 Enhanced removal of detergent and recovery of enzymatic activity following sodium dodecyl sulfate-polyacrylamide gel electrophoresis:use of casein in gel wash buffer McGrew,B.R.;D.M.Green https://doi.org/10.1016/0003-2697(90)90045-B
  22. J. Gen. Microbiol. v.138 Chemical modification studies of the active centre of Candida albicans chitinase and its inhibition by allosamidin Milewski,S.;R.W.ODonnell;G.W.Gooday
  23. Appl. Environ. Microbiol. v.60 Induction of chitin-binding proteins during the specific attachment of the marine bacterium Vibrio harveyl to chitin Mongotmery,M.T.;D.L.Kirchman
  24. J. Ferment. Technol. v.57 Purification and some properties of chitinase from Vibrio sp. Ohtakara,K.;M.Mitsutoni;Y.Ugida
  25. Phytophathol v.78 The role of chitinase of Serratia marcescens in biocontrol of Sclerotium rolfsii Ordenlich,A.;Y.Elad;I.Chet
  26. Appl. Microbiol. Biotechnol. v.36 Bioactivity of chitinolytic actinomycetes of marine origin Pisano,M.A.;M.J.Sommer;L.Taras
  27. Electrophoresis v.9 no.6 Improvement and simplification of low-background silver staining of proteins by using sodium dithionite Rabilloud,T.;G.Carpentier;P.Tarroux https://doi.org/10.1002/elps.1150090608
  28. Biotechnology and Bioengineering v.23 no.5 Conversion of the enzymatic hydrolysate of shellfish waste chitin to single-cell protein Revah-Moiseev,S.;A.Carroad https://doi.org/10.1002/bit.260230514
  29. J. Gen. Microbiol. v.134 Plant and bacterial chitinases differ in antifungal activity Roberts,W.K.;C.P.Selitrennikoff
  30. Plant and Soil v.75 no.1 The determination of soil chitinase activity:conditions for assay and ecological studies Rodriguez-Kabana,R.;G.Godoy,G.Morgan-Jones;R.A.Shelby https://doi.org/10.1007/BF02178617
  31. Appl. Environ. Microbiol. v.64 Characterization of chitinase C from a marine bacterium,Alterononas sp.strain O-7,and its corresponding gene and domain structure Tsujibo,H.;H.Orikoshi;K.Shiotani;M.Hayashi;J.Umeda;K.Miyamoto;C.Imada;Y.Okami;Y.Inamori
  32. Can. J. Microbiol. v.38 Purification,properties,and partial amino acid sequence from a marine Aeromonas sp.strain O-7 Tsujibo,H.;Y.Yoshida;K.Miyamoto;C.Imada;Y.Okami;Y.Inamori
  33. J. Plant. Physiol v.153 Partial characterization of the complex chitinolytic system in leek(Allium porrum L.) plants Vergauwen,R.;A.van Laere
  34. Appl. Environ. Microbiol. v.63 Purifitation and characterization of two bifunctional chitinase by Psudomonas aeruginos K-187 in a shrimp and crab shell powder medium Wang,S.L.;W.T.Chang
  35. J. Bacteriol. v.172 Chitinase system of Bacillus circulans WL-12 and importance of chitinase A1 in chitin degradation Watanabe,T.;W.Oyanagi;K.Suzuki;H.Tanaka
  36. J. Gen. Appl. Microbiol. v.2 Purification and characterization of chitinase and chitobiase produced by Aeromonas hydrophilla subsp Yabuki,M.;K.Mizushina;T.Amatatsu;A.Anda;T.Fujii;M.Shimada;M.Yamashita
  37. J. Biol. Chem. v.266 Chitin utilization by marine bacteria Yu,C.;A.M.Lee;B.L.Bassler;S.Roseman