Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Antitumor activities of Gamdutang aqua-acupuncture solution

감두탕 약침액의 암예방 효과에 관한 연구

  • Han, Sang-Hoon (Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Park, In-Kyu (Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Moon, Jin-Young (Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University) ;
  • Lim, Jong-Kook (Dept. of AM-Pointology, College of Oriental Medicine, Dongguk University)
  • 한상훈 (동국대학교 한의과대학 경혈학교실) ;
  • 박인규 (동국대학교 한의과대학 경혈학교실) ;
  • 문진영 (동국대학교 한의과대학 경혈학교실) ;
  • 임종국 (동국대학교 한의과대학 경혈학교실)
  • Published : 2000.03.20
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Abstract

Gamdutang aqua-acupuncture solution(GAS), Gamdutang water-extracted solution(GWS) and Degamdutang aqua-acupuncture solution(DGAS) were prepared and tested for potential antitumor activities. It was used three biomarkers (quinone reductase, omithine decarboxylase, glutathione) to test chemopreventive potentials of GAS, GWS, DGAS. GAS was potent inducer of quinone reductase activity in Hepalclc7 murine hepatoma cells in culture, whereas GWS is less potent. GAS, GWS and DGAS were significantly induced quinone reductase activity in cultured rat normal liver cell, Ac2F. Glutathione levels were increased about 1.8-fold with GAS, 1.0-1.1 fold with GWS, DGAS in cultured murine hepatoma hepaiclc7 cells. In addition glutathione s-transferase levels were increased with GAS, GWS and DGAS. The effects of GAS, GWS and DGAS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii was inhibited by GAS, GWS and DGAS at concentradons of $1{\times}$ and $5{\times}$. These results suggest that GAS has chemopreventive potential by inducing quinone reductase and quinone reductase activities, inhibition of ornithine decarboxylase activity, and increasing glutathione levels.

Keywords

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