Cloning and Sequence Analysis of the xyIL Gene Responsible for 4CBA-Dihydrodiol Dehydrogenase from Pseudomonas sp. S-47

  • 박동우 (Department of Microbiology and Biotechnology, and Research Institute for Genitic Engineering) ;
  • 이상만 (Department of Life Science) ;
  • 가종옥 (농업생명학과) ;
  • 김지경 (Dept. of Microbiology and Research Ins. of Genetic Eng.)
  • Published : 2002.12.01

Abstract

Pseudomonas sp. S-47 is capable of catabolizing 4-chlorobenzoate (4CBA) as carbon and energy sources under aerobic conditions via the mesa-cleavage pathway. 4CBA-dioxygenase and 4CBA-dihydrodiol dehydrogenase (4CBA-DD) catalyzed the degradation af 4CBA to produce 4-chlorocatechol in the pathway. In this study, the xylL gene encoding 4CBA-DD was cloned from the chromosomal DNA of Pseudomonas sp. S-47 and its nucleotide sequence was analyzed. The xylL gene was found to be composed of 777 nucleotide pairs and to encode a polypeptide of 28 kDa with 258 amino acid residues. The deduced amino acid sequence of the dehydrogenase (XylL) from strain S-47 exhibited 98% and 60% homologies with these of the corresponding enzymes, Pseudomonas putida mt-2 (XyIL) and Acinetobacter calcoaceticus (BenD), respectively. However, the amino arid sequences show 30% or less homology with those of Pseudomonas putida (BnzE), Pseudomonas putida Fl (TodD), Pseudomonas pseudoalcaligenes KF707 (BphB), and Pseudomonas sp. C18 (NahB). Therefore, the 4CBA-dihydrodiol dehdrogenase of strain S-47 belongs to the group I dehydrogenase involved in the degradation of mono-aryls with a carboxyl group.

Keywords

References

  1. Biochem. J. v.146 Purification and some properties of a soluble benzene-oxidizing system from a strain of Pseudomonas Axcell,B.C.;P.J.Geary
  2. Biochemical and Biophysical Research Communications v.260 no.1 Cis-2,3-dihydro-2,3,-dihydroxybiphenyl dehydrogenase and cis-1,2,-dihydro-1,2-dihydroxynaphthalene dehydrogenase catalyze dehydrogenation of the same range of substrates Barriault,D.;M.Vedadi;J.Powlowski;M.Sylvestre https://doi.org/10.1006/bbrc.1999.0706
  3. J. Bacteriol. v.175 Metabolism of dibenzothiophene and naphthalene in Pseudomonas strains:complete DNA sequence of an upper naphthalene catabolic pathway Denome,S.A.;D.C.Stanley;E.S.Olson;K.D.Young
  4. J. Bacteriol. v.178 Characterization and expression of the plasmid-born benD gene from Pseudomonas putida ML2,which codes for a $NAD^+$-dependent cis-benzene dihydrodiol dehydrogenase Fong,K.P.Y.;C.B.H.Goh;H.M.Tan
  5. J. Bacteriol. v.175 Gene components responsible for discrete substrate specificity in the metabolism of biphenyl(bph operon) and toluene Furukawa,K.;J.Hirose;A.Suyama;T.Zaiki;S.Hayashida
  6. J. Bacteriol. v.173 Potential DNA slippage structures acquired during evolutionary divergence of Acinetobacter calcoaceticus chromosomal benABC and Pseudomonas putida TOL pWW0 plasmid xylXYZ,genes encoding benzoate dioxygenase Harayama,S.;M.Rekik;A.Barioch;E.L.Neidle;N.Omston
  7. J. Bacteriol. v.169 Nucleotide sequencing and characterization of the genes encoding benzene oxidation enzymes of Pseudomonas putida Irie,S.;S.Doi;T.Yorifuji;M.Takagi;K.Yano
  8. J. Microbiol v.38 Microbial degradation of monohydroxybenzoic acid Karegoudar,T.B.;C.K.Kim
  9. J. Microbiol v.36 Cloning and expression in E.coli of the genes responsible for degradation of 4-chlorobenzoate and 4-chlorocatechol from Pseudomonas sp Kim.K.P;D.I.Seo.;D.H.Lee;Y.Kim;C.K.Kim
  10. J. Microbiol v.35 Characteristics of catechol 2,3-dioxygenase produced by 4-chlorobenzoate by Pseudomonas sp. Kim.K.P;D.I.Seo.;K.H.Min;J.O.ka;Y.K.Park;C.K.Kim
  11. FEMS Microbiol. Lett v.182 Cloning and characterization of a novel cis-naphthalene dihydrodiol dehydrogenase gene(narB) from Rhodococcus sp Kulakov,L.A.;C.C.R.Allen;D.A.Lipscomb;M.J.Larkin
  12. J. Bacteriol. v.179 Stereochemical course of two arene-cis-diol dehydrogenases specifically induced in Pseudomonas putida Morawski,B.;G.Casy;C.Illaszewicz,H.Griengl;D.W.Ribbons
  13. European Journal of Biochemistry v.204 no.1 Cis-diol dehydrogenase encoded by the TOL pWW0 plasmid xylL,gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the shortchain alcohol dehydrogenase superfamily Neidle,E.;C.Hartnett;L.N.Ornston;A.Bairoch;M.Rekik;S.Harayama https://doi.org/10.1111/j.1432-1033.1992.tb16612.x
  14. Moll. Cells v.10 Cloning and nucleotide sequence analysis of xylE gene responsible for meta-cleavage of 4-cholorocatechol from Pseudomonas sp. Noh,S.J.;Y.Kim;K.H.Min;T.B.Karegoudar;C.K.Kim;
  15. Appl. Environ. Microbiol. v.62 Cloning,sequencing,and expression of isopropylbenzene degradation genes from Pseudomonas sp.strain JR1:Identification of isopropylbenzene dioxygenase that mediates trichloroethene oxidation Pflugmacher,U.;B.Averhoft.;G.Gottschalk;
  16. Appl. Environ. Microbiol. v.65 Cis-chlorobenzene dihydrodiol dehydrogenase(TcbB) from Pseudomonas sp.strain P51,expressed in Escherichia coli DH5α(pTCB149).catalyzes enantioselective dehydrogenase reactions Raschke,H.;T.Fleischmann;J.R.van der Meer;H.P.E.Kohler
  17. J. Bacteriol. v.130 Purification and properties of cis-toluene dihydrodiol dehydrogenase from Pseudomonas putida Rogers,J.E.;D.T.Gibson
  18. Molecular cloning:A laboratory manual(2nd ed) Sambrook,J.;E.F.Fritisch;T.Maniatis
  19. J. Microbiol. Biotech v.8 A pathway for 4-chlorobenzoate degradation by Pseudomonas sp.S-47 and its degradation of 4-chlorobenzoic acid Seo,D.I.;J.C.Chae;K.P.Kim;Y.S.Kim;G.S.Lee;C.K.Kim
  20. J. Microbiol v.35 Isolation of Pseudomonas sp.S-47 and its degradation of 4-chlorobenzoic acid Seo,D.I;J.Y.Lim;Y.C.Kim;K.H.Min;C.K.Kim
  21. Biochemical and Biophysical Research Communications v.204 no.2 Toluene dioxygenase:purification of an ion-sulfur protein by affinity chromatography Subramanian,V.;T.N.Liu;W.K.Yeh;D.T.Gibson https://doi.org/10.1006/bbrc.1994.2546
  22. Appl. Environ. Microbiol. v.62 Characterization of active recombinant 2,3-dihydro-2,3-dibydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene(bphB) Sylvestre,Y.;Y.Hurtubise;D.Barriault;J.Bergeron;D.Ahmad
  23. J. Biol. Chem. v.267 Analysis of bph operon from the polychlorinated byphenyl-degrading strain of Pseudomonas pseudoalcaligenes KF707 Taira,K.;J.Hirose;S.Hayashida;K.Furukawa
  24. Journal of Biological Chemistry v.271 no.8 The broad substrate chlorobenzoated dioxygenase and cis-chloroben-zene dihydrodiol dehydrogenase of Pseudomonas sp.strain P51 are linked evolutionarily to the enzymes for benzene and toluene degradation Werlen,C.;H.P.E.Kohler;J.R.van der Meer https://doi.org/10.1074/jbc.271.8.4009
  25. J. Biol. Chem. v.264 Toluene degradation by Pseudomonas putida F1.Nucleotide sequence of the todC1C2BADE genes and their expression in Escherichia coli Zylsta,G.J.;D.T.Gibson