Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A Screening System for Environmental Mutagens by Means of Specific Locus Mutation of the Silkworm, Bombyx mori

누에의 특정좌위 돌연변이를 이용하는 변이원 검색계의 피검누에계통 선정 및 변이원 감수성

  • 김삼은 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 안미영 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 원형주 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 김종길 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 최지영 (농촌진흥청 농업과학기술원 잠사곤충부)
  • Published : 2000.03.01
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Abstract

A mutagenicity testing system using a specific locus mutation of Bombyx mori was introduced from National Institute of Genetics in Japan. In the system, mutagenicity could be detected by the egg color manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. The efficiency to detect mutagenicity of the system was examined and improved. To promote the sensitivity of the system to mutagens, eight varieties of Bombyx mori were tested for their sensitivity to two mutagens, EMS and MMC. Two varieties of the silkworm, N12 and C5, were finally selected as the most sensitive ones. The most sensitive development stage of the silkworm to mutagens was mid and late pupal stages for female and male, respectively. The system will be applied to test unknown mutagens after some more detail examination about its sensitivity.

Keywords

References

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