Changes of Physiological Activity of Mustard Leaf during Its Fermentation Period

  • Lim, Hyun-Soo (Department of Biotechnology, Yosu National University) ;
  • Yoo, Eun-Jeong (Department of Biotechnology, Yosu National University) ;
  • Choi, Myeong-Rak (Department of Biotechnology, Yosu National University)
  • Published : 2000.02.01

Abstract

In vitro cytotoxicity and antioxidative effects of water extracts prepared from Mustard Leaf Kimchi (MLK) during its fermentation period were investigated. The cytotoxicity against HepG2 (human hepatic cancer) by water extracts from the well fermented Mustard Leaf Kimchi was higher than others (fermented for 0 to 6 days at 18$^{\circ}C$), and IC50 of water extracts at the points of 6, 8, 10, and 14days during fermentation were 213.4, 99.2, 99.9, and 109.8${\mu}{\textrm}{m}$/ml, respectively. Antioxidative activity of water extracts from MLK during various fermentation periods was higher than that of blank distilled water. However, the antioxidative activity of well-fermented water extracts of MLK (fermented for 8-14 days) did not show any difference from that of others (fermented for 2-6 days). Thus, water extracts of well-fermented MLK (fermented during 8-14 days) significantly inhibited the growth of cancer cells in vitro, but little antioxidative activity was influenced by the various fermentation periods.

Keywords

References

  1. Official Methods of Analysis. (16th ed) no.37 Fruits and products acidity (titrable) of truit products. AOAC
  2. J. Microbiol. Biotechnol. v.6 Isolation of Leuconostoc mesenteroides subsp. mesenteroides DU-0608 with antibacterial activity from Kimchi and characterization of its bacteriocin. Cha, D. S.;D. M. Ha.
  3. Food Analysis(Standard) Cheigh, S. K.
  4. Crit. Rev. Food. Sci. Nutr. v.34 Biochemical, microbiological and nutritional aspects of Kimchi (Korean fermented vegetable products). Cheigh, H. S.;K. Y. Park.
  5. Sunchon Natl. Univ. Bull. v.9 Studies on the chemical components of Korean Brassica Juncea Cosson. Cho, Y. S.;S. K. Park.
  6. Kor. J. Food Sci. Technol. v.30 Changes of fermentation characteristics and sensory evaluation of Kimchi on different storge temperature. Choi, S. Y.;M. K. Lee;K. S. Choi.;Y. J. Koo;W. S. Park.
  7. J. Microbiool. Biotechnol. v.7 Plasmid-associated bacteriocim production by Leuconostoc sp. LAB145-3A isolated from Kimchi. Choi. Y. O.;C. Ann.
  8. Anal. Chem. v.31 no.426 Use of dinitrosalicyclic acid reagent for determination of reducing sugar Gail, L. M.
  9. J. Microbiol. Biotechnol. v.4 Indenitification of bacteriocin-producing lactic acid bacteria from Kimchi and partial characterization of their bacteriocin. Ha, D. M.;D. S. Cha;S. G. Han
  10. J. Microbiol. Biotechnol. v.8 Bacteriocin produced by Lactobacillus curvatus SEI isolated from Kimchi Kim, S. K.;E. J. Lee;K. Y. Park.;H. K. Jun
  11. J. Microbiol. Biotechnol. v.9 Improvement of Kimchi fermentation by using acid-tolerant mutant of Leuconostoc mesenteroides and aromatic yest Saccharomyces fermentation as starters. Kim, Y. C.;E. Y. Jung;S. G. Hong;K. T. Jung;S. M. Kang
  12. Biochimie v.70 Bacteriocins of lactic acid bacteria. Klaenhammer, T. R.
  13. J. Microbiol. Biotechnol. v.9 Identification and characterization of bacteriocin producing Lactic acid bacteria isolated from Kimchi Lee, H. J.;C. S. Park.;Y. J. Joo;S. H. Kim;J. H. Yoon;Y. H. Park.;I.K. Hwang;J. S. Ahn;T. I. Min
  14. J. Kor. Soc. Food Nutr. v.26 Antioxidative characteristics of isolated crude phenolics from soybean fermented foods (Doenjang) Lee, J. S.;H. S. Cheigh.
  15. Ph.D. Thesis Inhibitory effects of green-yellow vegetables of mutagenicity induced by various mutagens and on the frowth of human cancer cells. Lee, K. I.
  16. Ph.D. Thesis Studies on the antioxidative characteristics and antioxidative substances of Kimchi Lee, Y. O.
  17. J. Kor. Soc. Food. Nutr. v.24 The nutritional evaluation, and antimutagenic and anticancer efffects of Kimchi Park, K. Y.
  18. The sauerkrant fermentation. v.824 Peterson, C. S.;M. H. Albery
  19. Cancer Res. v.48 Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Scudiero, D. A.;R. H. Shoemaker;K. D. Paull;A. Monks;S. Tierney;T. H. Nofiziger;M. J. ns Curre;D. Seniff;M. R. Boyd.
  20. J. Kor. Soc. Food Nutr. v.26 Changes in chlorophylls and carotenoides of Mustard leaf Kimchi during fermentation and their antioxidative activities on the lipid oxidation. Song, E. S.;Y. S. Jeon;H. S. Cheigh.
  21. Kor. J. Food Sci. Technol. v.28 The effect of low molecular weight chitosan with and without other preservatives on the characteristics of Kimchi during fermentation Son, Y.M.;K.O. Kim;D. W. Jeon;K. H. Kyung
  22. Cancer Res v.48 Chemoprevention of N-nitrosomethylbenzylamine induced esophageal cancer in rats by the naturall occurring thioether, diallyl sulfide. Wargovich, M. J.;C. Wood;V. W. S. Eng;L. C. Stephens;K. Gray