Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of the HSV-1 (F) Glycoprotein B Gene in Insect Cells Infected by HcNPV Recombinant

  • Published : 2000.06.01
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Abstract

The Herpes simplex virus type 1 (HSV-1) glycoprotein B (gB) gene in the pHLA-21 plasmid was inserted into a baculovirus (Hyphantria cunea nuclear polyhedrosis virus) expression vector (lacZ-HcNPV) to construct a recombinant virus gB-HcNPV expressing gB. Spodoptera frugiperda cells infected with this recombinant virus synthesized and processed gB of approximately 120 kDa, which cross-reacted with the monoclonal antibody to gB. The recombinant gB was identified on the membrane of the insect cells using an immunofluorescence assay. Antibodies to this recombinant raised in mice recognize the viral gB and neutralized the infectivity of the HSV-1 in vitro. These results show that the gB gene has the potential to be expressed in insect cells. They also demonstrate that it is possible to produce a mature protein by gene transfer in eukaryotic cells, and indicate the utility of the lacZ-HcNPV-insect cell system for producing and characterizing eukaryotic proteins. Furthermore, the neutralizing antibodies would appear to protect mice against HSV. Accordingly, this particular recombinant protein may be useful in the development of a subunit vaccine.

Keywords

References

  1. J. Virol. v.67 The UL10 gene of herpes simplex virus 1 encodes a novel viral glycoprotein, gM, which is present in the virion and in the plasma membrane of infected cells Bains, J. D.;B. Roizman
  2. Nucl. Acids. Res. v.7 A rapid alkaline extraction procedure for screening recombinant plasmid DNA Birnboim, H. C.;J. Doly
  3. J. Gen. Virol. v.68 Specificity of the immune response of mice to herpes simplex virus glycoprotein D and B constitutively expressed on L cell lines Blacklaws, B. A.;A. A. Nash;G. Darby
  4. J. Gen. Virol. v.71 Immunological memory to herpes simplex virus type 1 glycoprotein B and D in mice Blacklaws, B. A.;A. A. Nash
  5. Protein Methods(2nd ed.) Bollag, D. M.;M. D. Rozycki;S. J. Edelstein
  6. Eur. J. Biochem. v.46 A film detection method for tritrum labeled proteins and nucleic acids in p0olyacrylamide gels Bonner, W.;R. Laskey
  7. Virol. v.133 Nucleotide sequence specifying the glycoprotein gene, gB, of herpes simplex virus type 1 Bzik, D. J.;B. A. Fox;N. A. DeLuca;S. Person
  8. J. Virol. v.62 Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion Cai, W.;B. Gu;S. Person
  9. Proc. Natl. Acad. Sci. UDA v.84 Expression of herpes simplex virus 1 glycoprotein B by a recombinant vaccinia virus and protection of mice against lethal herpes simplex virus 1 infection Cantin, E. M.;R. Eberal;J. L. Baldick;B. Moss;D. E. Willy;A. L. Notkins;H. Openshaw
  10. Immunol v.49 Protective immunization of mice with specific HSV-1 glycoproteins Chan, W. L.
  11. J. Microbiol. Biotechnol. v.4 Nucleotide sequence analysis of the RNA-dependent RNA polymerase gene of infectious pancreatic necrosis virus DRT strain Chung, H. K.;S. H. Lee;S. Y. Kim;H. H. Lee
  12. J. Virol v.61 Amino-terminal sequence synthesis, and membrane insertion of glycoprotein B of herpes simplex virus type 1 Claesson-Welsh, L.;P. G. Spear
  13. J. Virol. v.52 Evidence for post translational glycosylation of a nonglysosylated precursor protein of herpes simplex virus type 1 Compton, T.;R. J. Courtney
  14. Virol. v.122 Nucleotide sequences of herpes simplex virus type 1(HSV-1) affecting virus entry, cell fusion, and production of glycoprotein gB(VP7) DeLuca, N.;D. J. Bzik;V. C. Bond;S. Person;W. Snipes
  15. J. Gen. Virol. v.2 Characterization of herpes simplex strains differing in their effects on social behavior of infected cell Ejecito, P. M.;E. D. Kieff;B. Roizman
  16. Vaccine v.14 Vaccination with a cocktail of seven recombinantly expressed HSV-1 glycoproteins protects against ocular HSV-1 challenge more efficiently thanb vaccination with any individual glycoprotein Ghiasi, H.;A. B. Nesburn;S. L. Wechsler
  17. Virus Res. v.22 Expression of glycoprotein B of herpes simplex virus type 1 in insect cells: Initial analysis of its biochemical and immunological properties Ghiasi, H.;R. Kaiwar;A. B. Nesburn;S. L. Wechsler
  18. J. Virol. v.68 Characterization of BVaculovirus-expressed HERPES SIMPLEX VIRUS TYPE 1 GLYCOPROTEIN k. Ghiasi, H.;S. Slanina;A. B. Nesburn;S. L. Wechsler
  19. Proc. Natl.Acad. Sci. USA v.72 Colony hybridization: A method for the isolation of cloned NDAs that contain a specific gene Grunstein, M.;D. Hogness
  20. J. Microbiol. Biotechnol. v.8 Expression and characterization of recombinant E2 protein of Hepatitis C virus by insect cell/Baculovirus expression system Han, B. K.;B. Lee;M. K. Min;K. H. Jung
  21. J. Virol. v.62 Monoclonal antibodies define a domain on herpes simplex virus glycop0rotein B involved in virus penetration Highlander, S. L.;W. Cai;S. Person;M. Levine;J. C. Glorioso
  22. Virol. v.64 Abundant expression of Herpes simplex virus glycoprotein gB using an adenovirus vector Johnson, D. C.;G. Ghosh-Choudhury;J. R. Smiley;L. L. Fallis;R. L. Graham
  23. J. Kor. Soc. Virol. v.26 Cloning of thymidine kinase gene of Herpes simplex virus type-1 Kang, H.;K. J. Park;S. C. Cha;S. Y. Kim;K. S. Yang;N. J. Kim;H. H. Lee
  24. J. Immunol. v.115 Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent preparations of the interaction of antibody-antigen complexes with protein A. Kessler, S.
  25. Vaccine v.7 Immunogenicity of herpes simplex virus glycoprotein gB-1 related protein produced in yeast. Kino, Y.;C. Nozaki;H. Mizuno;R. Mori
  26. Hanguk J. Genet. Engin. v.2 Replication and cloning of Hyphantria cunea nuclear polyhedrosis virus in Spodoptera frugiperda cell line Lee, H. H.
  27. J. Microbiol. Biotechnol. v.8 Construction of a Baculovirus expression system using H. cunea nuclear polyhedrosis virus for eukaryotic cell. Lee, H. H.;B. J. Kang;K. J. Park.
  28. J. Gen. Virol. v.69 complementation and partial characterization of temperature-sensitive mutants of the Baculovirus Hyphantria cunea nuclear polyhedrosis virus Lee, H. H.;K. W. Lee
  29. J. Microbiol. Biotechnol. v.8 Construction of a Baculovirus Hyphantria cunea NPV insecticide containing the insecticidal protein gene of Bacillus thuringiensis subsp. kurstaki HD1 Lee, H. H.;E. S. Moon;S. T. Lee;S. H. Hwang;S. C. Cha;K. H. Yoo
  30. J. Kor. Soc. Virol. v.20 Restriction mapping of the DNA genome of Hyphantria cunea nuclear polyhedrosis virus Lee, H. H.;H. J. Lee;K. H. Yoo
  31. J. Microbiol. Biotechnol. v.9 Construction of a transfer vector containing gX signal sequence of Pseudorabies virus and a recombinant baculovirus Lee, H. H.;H. Kang;J. W. Jung;B. J. Kang;J. Y. Song
  32. J. Virol. v.27 Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus Lee, H. H.;L. K. Miller
  33. J. Biochem. Mol. Biol. v.32 Cloning and characterization of the psbEF gene encoding cytochrome b-559 of the Panax ginseng photosystem II reaction center Lee, W. K.;D. S. Park;G. S. Tae
  34. J. Mol. Biol. v.53 Calcium dependent bacteriophage DNA phage infection Mandel, M.;A. Higa
  35. J. Virol v.64 Protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein B constitutively expressed in human cells with a BK virus episomal vector Manservigi, R.;P. R. Grossi;M. Gualandri;P. G. Balboni;A. Marchini;P. Rimessi;D. DeLuca;E. Cassai;G. Barbanti-Brodano
  36. J. Immunol. v.138 The mechanism of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein D: Clearance of local infection Martin, S.;B. T. Rouse
  37. cell-to-cell spread and cell fusion. Virol. v.186 Domains of Herpes simplex virus type 1 glycoprotein B that function in virus penetration, Navarro, D.;P. Paz;L. Pereira
  38. J. Virol. v.68 Vaccine therapy for ocular Herpes simplex virus(HSV) infection: Periocular vaccination reduces spontaneous ocular HSV type 1 shedding in latently infected rabbits Nesburn, A. B.;R. L. Burke;H. Ghiasi;S. Slanina;S. Bahri;S. L. Wechsler
  39. A Laboratory Manual. Baculovirus Expression Vectors O'Reilly, D. R.;L. K. Miller;V. A. Luckow
  40. J. Virol. v.61 Expression of cell associated and secreted forms of herpes simplex virus type 1 glycoprotein gB in mammalian cells Pachl, C.;R. L. Burke;L. L. Stuve;L. Sanchez-Pescador;G. Van Nest;D. Masiarz;D. Dina
  41. J. Virol. v.41 Glycoprotein gE of herpes simplex virus type 1: Effects of anti-gE on virion infectivity and on virus induced Fc-binding receptors Para, M. F.;R. B. Baucke;P. G. Spear
  42. J. Virol. v.53 Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: Primary sequence and predicted protein structure of the wild type and monoclonal antibody-resistant mutants Pellett, P. E.;K. G. Kousoulas;L. Pereira;B. Roizman
  43. Virol. v.186 In vitro characterization of the HSV-1 UL53 gene product Ramaswamy, R.;T. C. Holland
  44. Amer. J. Hygiene v.27 A simple method of estimating fifty per cent endpoints. Reed, L. J.;H. Munch
  45. Molecular Cloning; A Laboratory Manual. Sambrook, L.;E. F. Fritsch;T. Maniatis
  46. Proc. Natl. Acad. Sci. USA v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A. R. Coulson
  47. J. Virol. v.46 Molecular engineering of the Autographa californica nuclear polyhedrosis virus genome: Deletion mutations within the polyhedrin gene Smith, G. E.;M. J. Fraser;M. D. Summers
  48. J. Mol. Biol. v.69 Detection of specific sequences among DNA fragments separated by gel electrophoresis Southern, E. M.
  49. J. Virol. v.17 Membrane proteins specified by herpes simplex virus type 1. I. Identification of four glycoprotein precursors and their products in type 1 infected cells Spear, P. G.
  50. The Herpesviruses v.3 Glycoproteins specified by herpes simplex viruses Spear, P. G.;Roizman, B.(ed.)
  51. Taxas Agricultural Experimentation Station A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures Summers, M. D.;G. E. Smith
  52. Vitro v.13 The establishment of two cell lines from insect Spodoptera frugiperda (Lepidoptera: Noctuidae) Vaughan, J. L.;R. H. Goodwin;G. J. Tomkins;P. McCarvely
  53. J. Virol. v.44 Endo-β-n-acetyl-glucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC. Wenske, E. A.;M. W. Bratton;R. J. Courtney