Effect of Enzymatic Hydrolysate from Egg Yolk Protein on the Activity of Antioxidative Enzyme in Cultured Hepatocytes (Chang)

배양 간세포 (Chang)에서 황산화작용 및 항상화요소 활성에 미치는 계란 놀느자 단백질 가수분해물의 영향

  • Published : 2000.10.01

Abstract

Normally, aerobic cells are protected from the damage of free radicals by antioxidative enzymes such as catalase, superoxide dismutase (SOD), glutathione (GSH) peroxidase and GSH-S-transferase. In this study, we have investigate the effect of egg yolk protein hydrolysates on antioxidative activity and the activity of antioxidative enzyme in cultured hepatocytes (Chang). Without the pretreatment with hydrolysate, about 50% of the hepatocytes were killed within 2h by 225$\mu$M tert-butyl hydroperoxide (t-BHP). By contrast, fewer than 20% of the 5 K hydrolysate (permeate from 5 kDa membrane and not passed through 1 kDa membrane)-pretreated hepatocytes were killed by the same concentrations of t-BHP. In addition, the activities of catalase, GSH peroxidase and GSH-transferase were significantly increasing with the treatment of 5 K hydrolysate. These results suggest that 5 K hydrolysate exerts antioxidative effect by increasing activity of antioxidative enzymes.

References

  1. Methods of enzymatic analysis. Aebi, H.
  2. Life Sci. v.52 Thymic peptide protects vacular endotherial cells from hydrogen peroxide-induced oxidant injury. Benjamin, H. S.;L. L. Li;P. Yoon
  3. Korean J. Immun. v.19 Effects of pilose antler on lipid peroxidation and antioxidative enzymes in cultured liver cells. Choi, M. J.;M. W. Choi;Y. K. Kim;C. H. kim;J. Y. Moon;K. S. Nam
  4. J. Free Rad. Biol. Med. v.2 Intracellular proteolytic systems may function ad secondary antioxidant defenses : a hypothesis. Davies, K. J. A.
  5. Biochem. Biophys. Res. Commun. v.121 Extracellular calcium protects isolated rat hepatocytes from injury. Fariss, M. W.;K. Olafsdottir;D. J. Reed
  6. J. Biochem. v.249 Glutathion-S-transferase : the first enzymatic step mercapturic acid formation. Habig, W. H.;M. J. Pabst;W. B. Jabby
  7. Arch. Biochem. Biophy. v.246 Oxygen free radicals and iron in relation to biology and medicine: some problems and concepts. Halliwell, B;J. M. C. Gutteridge
  8. Chem. Biol. Interact. v.59 Metal-induced DNA damage and repair in human diploid fibroblasts and Chinese hamster ovary cells. Hamilton-Koch, W.;R. D. Snyder;J. M. Lavelle
  9. "Glutathion peroxidase" enzymatic basis of detoxication I. Jakoby, W. B.
  10. Active oxygen-molecular mechanism of its production, scavenging and effect in organism. Aging Kator, K.;Nakano, M.(ed.)
  11. Biochem. Biophys. Res. Commun v.71 Glutathione perxidase activity in selenium-deficient rat liver. Lawrence, R. A.;R. F. Burk
  12. J. Biol. Chem. v.261 Antioxidant protection of phospholipid bilayer by α-tocopherol : Control of α-tocopherol status and lipid perxidation by ascorbic acid and glutathione. Liebler, D. C.;D. S. Kling;D. J. Reed
  13. Arch. Biochem. Biophys. v.269 Tertbytyl hydroleroxide kills cultured hepatocytes by peroxidizing membrane lipids. Masaki, N.;M. E. Kyle;A. L. Farber
  14. Arch. Biochem. Biophys. v.270 Mitochondrial damage as a mechanism of cell injury in the killing of cultured hepatocytes by tert-butyl hydroperoxide. Masaki, N.;M. E. Kyle;M. E. Serroni;A. L. Farber
  15. J. Biol. Chem. v.244 Superoxide dismutase : Enzymatic function erythrocuprein (hematocuprein). McCord, J. M.;I. Fridovich
  16. Drug Meta. Rew. v.27 Mechanism of the formation and disposition of reactive metabolites that can cause acute liver injury. Nelson, S. D.
  17. Methods in Enzymology. v.105 Oberley, L. W.;P. R. Spitz
  18. Br. J. Cancer. v.55 The formation, identification, and significance of DNA-protein cross-links in mammalian cells. Oleinick, N. L.;S. Chiu;N. Ramarkrisshnan;L. Xue
  19. Agric. Biol. Chem. v.45 A novel type of antioxidant isolated from leaf wax of eucalyptus leaves. Osawa, T.;M. Namiki
  20. Anal. Biochem. v.42 Revaluation of assay methods and establishment of kit for superoxide dismutase activity. Oyanagui, Y.
  21. J. Lab. Clin. Med. v.70 Studies on quantative an qualitative characterization of erythrocyte glutathione peroxidase. Paglia, D. E.;W. N. Valentine
  22. Kor. J. Life Sci. v.10 Antioxidative effect of enzymatic protein hydrolysate from lecithin-free yolk. Park, P. J.;W. K. Jung;Y. R. Choi;S. K. Kim
  23. Arch. Biochem. Biophys. v.254 Relationship between cellular calcium and vitamin E metabolism during protection against cell injury. Pascoe, G. A.;D. J. Reed
  24. Arch. Biochem. Biophys. v.268 The multicatalytic proteinase of mammalian cells. Rivett, A. J.
  25. Biochem. Pharm. v.43 Dissociation of a new glutathion S-transferase activity in human erythrocytes. Schroder, K. R.;E. Hallier;H. Peter;H. M. Bolt
  26. Free Radic. Med. v.21 Oxidative stress during viral infection. Schwarz, K. B.
  27. J. Immunol. Methods. v.157 An improved MTT assay. Sladowski, D.;S. J. Steer;R. H. Clothier;M. Balls
  28. Anal. Biochem. v.150 Measurement of protein using bicichoninic acid. Smith, P. K.;R. I. Krohn;G. T. Hermanson;A. K. Mallia;F. H. Garther;M. D. Provenzano;E. K. Fujimoto;B. J. Goeke;D. C. Klent
  29. Nippon Eiyo Shokuryo Gakkaishi v.42 Blood pressure reduction and vasolilatory effects in vivo of peptides orignation from sardine mucle. Stutsuna, K.;K. Osajima
  30. Carcinogenicity and mutagenicity of some metals and their compounds. v.71 Sunderman, F. W. Jr.
  31. Biosci. Biotechol. Biochem. v.59 Antioxicative activity of hop bitter acids and their analogues. Tagashira, M.;M. Watanabe;N. Uemitsu
  32. Nippon shokuin Kogyo Gakkaishi v.26 Antioxidative activities of protein hydrolysates. Yamaguchi, N.;Y. Tokoo;M. Fujimaki
  33. Biochem. Pharmacol. v.33 Interrelation between lipid peroxidation other hepatotoxic events. Younes, M.;C. P. Siegers