한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제28권1호
  • /
  • Pages.21-25
  • /
  • 2000
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Serratia marecscens에서 maltose 대사를 촉진하는 유전자의 클로닝 해석

Analysis and cloning of the gene involved in activation of maltose metabolism in Serratia marcescens.

  • 이승진 (동아대학교 생명자원과학부) ;
  • 유주순 (동아대학교 생명자원과학부) ;
  • 김혜선 (동아대학교 생명자원과학부) ;
  • 이상철 (동아대학교 생명자원과학부) ;
  • 정수열 (동주대학 식품영양과) ;
  • 최용락 (동아대학교 생명자원과학부)
  • 발행 : 2000.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

We have got several clones from Serratia marcescens which stimulated the cells to use maltose as a carbon source in Escherichia. coli TP2139 ( lac, crp). One of the cloned genes, pCKB17, was further analyzed. In order to find whether the increased expression of the gent was under the direction of maltose metabolism, we constructed several recombinant subclones. We have found that the clone, pCKB17AV, codes maltose metabolism stimulation(mms) gene. E. coli transformed with the cloned gene showed increase in the activity of maltose utilzation, The recombinant proteins expressed by multicopy and induction with IPTG, one polypeptide of 29-kDa, was confirmed by SDS-PAGE. The overexpression of maltose-binding proter protein in the presence of mms gene was confirmed by Western blot analysis. Southern hybridization analysis confirmed that the cloned DNA fragment was originated from S. marcescens chromosomal DNA.

키워드

참고문헌

  1. Escherichia coli and Salmonella typhymurium: cellular and moleular biology The lactose operon Beckwith, J.;Neidhardt, F. C(et al.)(ed.)
  2. Anal. Biochem. v.72 A rapid and sensitive method of the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M.
  3. J. Bacteriol. v.171 Cloning and sequencing of an Escherichia coli gene, nlp, highly homologous to the ner genes of bacteriophages Mu and DIO8 Choi, Y. L.;T. Nishida;M. Kawamukai;R. Utsumi;H. Sakai;T. Komano
  4. Science v.224 Cyclic AMP receptor protein: role of transcriptional activation De Crombrugghe;B. S. Busby;H. Buc
  5. FEMS Microbiol. Lett. v.60 Cloning of the 52-kDa chitinase gene from Serratia marcescens KCTC 2172 and its proteolytic cleavage into an active 35-kDa enzyme Gal, S. W.;J. Y. Choi;C. Y. Kim;Y. H. Cheong;Y. J. Choi;J. D. Bahk;M. J. Cho
  6. Proc. Natl. Acad. Sci. USA v.51 The role of adenylic acid in the activation of photophorylase Helmreich, S.;C. F. Cori
  7. J. Bacteriol. v.173 Nucleotide sequence and characterization of the sfs1 gene: the sfs1 gene is involved in the $crp^*$ dependent mal gene expression of E. coli Kawamukai, M.;R. Utsumi;K. Takeda;A. Higashi;H. Matsuda;Y. L. Choi;T. Komano
  8. J. Life. Science v.9 no.1 Cloning and expression of Serratia marcescens Coenzyme A(CoA) transferase gene in E. coli Kim, H. S.;J. S. Yoo;Y. G. Kim;C. H. Chung;Y. L. Choi
  9. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemmli, U. K.
  10. Mol. Gen. Genet. v.198 A change of threonine 266 to isoleucine in the lac permease of Escherichia coli diminishes the transport of lactose and increases the transort of maltose Markgref, M.;H. Bocklage;B. Muller-Hill
  11. Eur. J. Biochem. v.252 no.2 Primary cause of mortality in the army warm larve simultaneously parasitized by parasitic wasp and infected with bacteria Matsmoto, H.;H. Noguchi;Y. Hayakawa
  12. Molecular Cloning: a laboratory manual(2nd ed.) Sambrook, J.;E. F. Fritsch;T. Maniatis
  13. Proc. Natl. Acad. Sci. USA v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;S. Nicklen;A. R. Coulson
  14. Escherichia coli and Salmonella typhymurium: cellular and molecular biology The maltose regulon Schwarz, M.;Neidhardt, F. C(et al.)(ed.)
  15. J. Mol. Biol. v.98 Detection of specific sequences among DNA fragments separated by gel electrophoresis Southern, E. M.
  16. Proc. Natl. Acad. Sci. USA v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some application Towbin, H.;T. Staechelin;J. Gordon
  17. Biochim. Biophysics. Acta. v.39 The characterization of the pathway of maltose utilization by Escherichia coli 1. Purification and physical chemical properties of the enzyme amylomaltase Wiesmeyer, H.;M. Cohen
  18. Kor. J. Life Science v.8 Analysis and cloning of cAMP receptor protein gene in Serratia marcescens Yoo, J. S.;H. S. Kim;J. H. Moon;S. Y. Chung;Y. L. Choi