Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Screening and Identification of an Inulinase Producing Microorganism and Optimal Condition for the Enzyme Production

Inulinase 생산균주의 분리.동정 및 효소 생산최적조건

  • Published : 2000.06.01
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Abstract

In an attempt to develop an unique enzyme (inulinase) for fructan utilization. bacterial strains were isolated [yom soil. Stram 96-11 secreting inulinase o[ high activity was tentatively identificated as Arthrobacter protophmmiae/ranwsus. The optimum culture conditions o[the slnin for the production of the inulinase were as follow: inorganic saIl basal medium contained sources fl % (w/v) inulin, 1 % (w/v) tryptone, and 1 % (w/v) $NH_4Cl$]. $35^{\circ}C$, initial pH 7.5. aeration 1 vvm and agitation 200 rpm.

국내 부존자원인 inulin을 이용하여 fructose syrup 또는 oligosacharide를 생산한는 효소를 발굴하기 위하여 전국적으로 토양 등 시료를 채취하여 inulinase 생성 미생물을 분리하였으며 inulinase 활성이 높은 96-1 균주를 동정한 결과 그람 양성균인 Arthrobacter protophormiae/ramosus인 것으로 잠정 동정되었다 Inulinase 생산을 위한 최적 배지는 무기염류 기초배지에 각 1%(w/v)의 inulin tryptone. $NH_4Cl$이 함유된 배지였으며 퇴적 발효조건은 $35^{\circ}C$초기 pH 7.5 통기량 1vvm 교반속도 200rpm이었다.

Keywords

References

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