KSBB Journal

  • 제15권1호
  • /
  • Pages.1-8
  • /
  • 2000
  • /
  • 1225-7117(pISSN)
  • /
  • 2288-8268(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

포집된 Bifidobacteria의 생존력 증대를 위한 세포포집재료의 선별기술 개발

Development of the Selection Technique of Entrapment Materials for the Viability Improvement of Entrapped Bifidobacteria

  • 이기용 (인하대학교 생물공학과) ;
  • 우창재 (인하대학교 생물공학과) ;
  • 배기성 (인하대학교 생물공학과) ;
  • 허태련 (인하대학교 생물공학과)
  • 발행 : 2000.02.01
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초록

The diffusion effect of simulated gastric juices into the various alginate vessel containing each biopolymer such as 0.3% soluble starch, whey, corn starch, agar, locust bean gum, guar gum, gum arabic, pectin, gelatin and 0.15% xanthan gum was tested by measuring the change of pH in the vessel. The degree of viability of bifidobacteria entrapped in each bead containing biopolymers was corresponded with the degree of diffusion inhibition of hydrogen into the each vessel. Therefore, The determination of diffusion inhibition of simulated gastric juices into the various vessel by measuring the change of pH in the vessel may be effectively used as the simple method to select the optimal entrapment lattice for the improvement of bifidobacteria viability. Bifidobacteria entrapped in alginate bead containing 0.15% xanthan gum whose lattice showed the lowest hydrogen diffusion were more significantly tolerant against bile salts and hydrogen peroxide than untrapped bifidobacteria. It was also observed that the viability of bifidobacteria entrapped in bead was nto nearly changed in milk adjusted pH 4.5 with organic adids at $4^{\circ}C$ for 10 days. Therefore, use of alginate containing 0.15% xanthan gum as a cell matrix for entrapping bifidobacteria was expected to improve the viability of bididobacteria in fermented milk products and develop the high value-added products.

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