Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Vital and Acrosomal Staining of Bovine Spermatozoa

소 정자의 생존율 및 첨체반응 검사를 위한 간단한 염색법

  • 김계성 (미국펜실배니아대학교 메디칼센터) ;
  • 이병천 (서울대학교 수의과대학) ;
  • 황우석 (서울대학교 수의과대학)
  • Published : 2000.04.01
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Abstract

The object of this study was to find simple and effective methods for the speculation of vitality and scorsome status of bovine spermatozoa. The eosin-nigrosin staining, trypan blue staining, and naphthol yellow S-erythrosin B staining was ofter used for the speculation of vitality and/or acrosome status of bovine spermatozoa, respectively. This study has shown that the combined trypan blue-naphthol yellow S-erythrosin B staining is more accurate and effective for the examination of acrosome status and vitality of bovine spermatozoa.

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