Sequence Analysis of the Coat Protein Gene of a Korean Isolate of Iris Severe Mosaic Potyvirus from Iris Plant

  • Park, Won-Mok (National Research Laboratory for GMO Safety Assessment, Graduate School of Biotechnology, Korea University) ;
  • Lee, Sang-Seon (Plant Virus GenBank, Department of Horticultural Science, College of Natural Science, Seoul Womens University) ;
  • Park, Sun-Hee (Plant Virus GenBank, Department of Horticultural Science, College of Natural Science, Seoul Womens University) ;
  • Ju (Plant Virus GenBank, Department of Horticultural Science, College of Natural Science, Seoul Womens University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Department of Horticultural Science, College of Natural Science, Seoul Womens University)
  • Published : 2000.02.01

Abstract

The coat protein gene of iris severe mosaic potyvirus, which was isolated in Korea, ISMV-K, from iris plant was cloned and its nucleotide sequence was determined. The coat protein of the virus contained 252 amino acid residues, including five potential N-glyxosylation site motifs. The coat protein of ISMV-K has 99.1% and 98.4% sequence identities with those of the Netherlands isolate of ISMV (ISMV-Ne) form crocus for the nucleotide and amino acids, respectively. The coat protein of ISMV-K has 50.4% to 60.3% nucleotide sequence identities and 47.3% to 55.7% amino acid identities with those of other 21 potyviruses, indicating ISMV to be a distinct species of the genus. The coat protein of ISMV-K was closely related with bean yellow mosaic virus and clover yellow vein virus in the phylogenetic tree analysis among the potyviruses analyzed. ISMV was easily and reliably detected from virus-infected iris leaves by RT-PCR with a set of the virus-specific primers.

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