Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Transglycosylation Reaction on Cellobiose by Dextansucrase of Leuconostoc mesenteroides B512FMC/6HG8

Leuconostoc mesenteroides B512FMC/6HG8가 생산하는 Dextransucrase에 의한 Cellobiose의 당전이반응

  • 강현록 (부경대학교 식품생명공학부) ;
  • 양지영 (부경대학교 식품생명공학부) ;
  • 이현규 (한양대학교 식품영양학부)
  • Published : 2000.10.01
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Abstract

The transglycosylation reaction by dextansucrase from Leuconostoc mesenteroides B512FMC/6HG8 was investigate with cellobiose as an acceptor molecule and sucrose as a donor. he optimal conditions of transglycosylation on cellobiose were found that the ration of sucrose to cellobiose was 3:1, the amount of enzyme was 2U/mL, the ionic strength of buffer was 25 mM, pH was 5.0 and reaction temperature was $25^{\circ}C$. also, acceptor products of cellobiose by transglycosylation were a series of oligosaccharides showing the degree of plymenzation of 6.

Cellobiose에 당전이 효소인 dextransucrase를 여러 가지 조건별로 반응시켜 올리고당의 생성경향을 알아보았다. Cellobiose에 대한 acceptor 반응의 최적조건은 cellobiose와 surcose의 비율은 3:1, 효소의 양은 2 U/mL, buffer의 이온강도는 25 mM, pH는 5, 반응온도는 $25^{\circ}C$로 나타났다. Cellobiose의 acceptor products는 종합도 6까지 생성되었으며, 구조는 2-O-isomaltodextrinyl cellobiose로 추정하였다.

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References

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