한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제29권5호
  • /
  • Pages.935-942
  • /
  • 2000
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

갈화가 에탄올을 투여한 흰쥐의 지질과산화와 알코올 대사효소의 활성도에 미치는 영향

Effects of Flower of Pueraria lobata on Lipid Peroxidation and Activities of Alcohol Metabolic Enzymes in Alcohol-treated Rats

  • 이정숙 (고신대학교 식품영양학과) ;
  • 김나영 (동아대학교 식품영양학과) ;
  • 이경희 (동아대학교 식품영양학과) ;
  • 김갑순 (경남정보대학 식품영양과) ;
  • 박희준 (상지대학교 응용식물과학부) ;
  • 최종원 (경성대학교 약학과) ;
  • 김석화 (동아대학교 식품영양학과)
  • 발행 : 2000.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

갈화(flower of Pueraria lobata) 분획 및 활성 성분의 급여가 아급성 알코올 중독된 흰쥐에서의 해독에 미치는 영향을 연구할 목적으로, 25% alcohol을 6주간 투여하여 아급성 알코올 중독상태를 유발한 흰쥐를 3주 더 사육하면서 혈액중 지질 함량의 변동, 알코올 대사계 효소의 활성을 비교 검퇀 결과는 다음과 같다. 알코올 단독 급여군은 대조군에 비하여 체중이 감소되었으며, 알코올-갈화 tectorigenin 급여군의 체중증가량은 대조군 수준에는 미치지 못하였으나, 알코올 단독 급여군보다는 유의적인 회복을 나타냈다. 알코올 단독 급여군의 총지방, 중성 지방, 인지질 함량이 대조군에 비하여 중가하엿고, 알코올-갈화 tcctorigcnin 급여군과 알코올-갈화 kaikasapomin III 급여군의 총지방, 주성지방 함량은 대조군 수준에는 미치지 못하였으나, 알코올 단독 급여군에 비하여 유의적으로 회복되었다. 알코올 단독 급여군의 total cholesterol, LDL-cholesterol, VLDL-cholesterol 함량, AI는 대조군에 비하여 증가하였고, 알코올-갈화 tectorigcnin, kaikasaponin III 급여군은 대조군 수준에는 미치지 못했으나, 알코올 단독 급여군에 버해 유의적으로 회복되았다. 알코올 단독 급여군의 HDL-cholcsterol의 함량은 대조군에 비하여 감소하던 것이 알코올-갈화 tectorigenin 급여군과 알코올-갈화 kaikasaponin III 급여군에서는 대조군 수준에서는 미치지 못하나, 알코올 단독 급여군에 비하여 증가하였다. 알코올 급여 시 cytochrome P 450, AH와 AD활성은 대조군에 비하여 증가했으나, 알코올-갈화 tectorigenin 급여군과 알코올-갈화 kaikasaponin III 급여군의 경우 알코올 단독 급여군보다 낮게 나타났다. 알코올 급여시 ADH활성이 증가하였으며 알코올-갈화 tectorigenin 급여군과 알코올-갈화 kaikasponin III 급여군은 알코올 단독 급여군보다 높게 나타났다. MEOS의 활성은 알코올 급여 시 대조군에 비하여 중가를 보였고, 알코올-갈화 tectorigenin 급여군에서는 알코올 단독 급여군보다 유의적인 증가를 나타냈다. Catalase의 활성은 각 군간의 유의적인 차이를 볼 수 없었다. ALDH의 활성은 알코올 단독 급여군은 대조군의 활성에 비하여 감소되었으나, 알코올-갈화 tectorigenin 급여군과 알코올-갈화 kaikasaponin III 급여군에서 알코올 단독 급여군보다 유의적인 활성증가를 보였다. 이상의 결과를 종합해 볼 때 갈화로부터 분리된 tectorigenin과 kaikasaponin III는 아급성 알코올 중독된 흰쥐 간의 free radical 생성계 효소를 억제시켜 알코올로 인한 간손상을 회복시키고, 알코올 대사 효소계에 관여하여 해독작용에 영향을 미침으로써 알코올 해독에 도움을 줄 수 있을 것으로 사료된다.

This study was designed to investigate the effect of flower of Pueraria lobata on liped peroxidation and activities of alcohol metabolic enzymes in alcohol-treated rats. Male Spra gue-Dawley rats were given 25% ethanol (Alcohol), 25% ethanol and 5 mg tectorigenin/kg B.W.(Alc.-Tec), 25% ethanol and 5mg kaikasaponin III/kg B.W. (Alc-Kai). The contents of serum total lipid, triglyceride and phospholipid were increased by ethanol treatment and were lower in the Alc.-Tec and Alc.-Kai group than in the Alcohol group. Decreased serum HDL-cholesterol by alcohol treatment was recovered by tectorigenin and kaikasaponin III. Microsomal cytochrome P-450, aniline hydroxylase and aminopyrine N-demethylase activities were increased by ethanol and were lower in the Alc. Tec and Alc.-Kai group than in the Alcohol group. Activity of hepatic alcohol dehydrogenase was increased by ethanol and was higher in the Alc.-Tec and Alc.-Kai group than in the Alcohol group. Microsomal ethanol oxidizing system activity was higher in Alc.-Tec group than in the other group. No significant difference was found in catalase activity among treatment groups. These data indicate that tectorigenin and kaikasaponin III were effected alcohol metabolic enzyme system and the liver damage associated with chronic ethanol consumption.

키워드

참고문헌

  1. Lab. Invest. v.50 Biology of disease. Alcoholism and aldehydeism : new biomedical concepts Warturg, J.P.;Buhler, R.
  2. Nutritional biochemistry and metabolism with clinical applications Linder, M.C.
  3. Clin. Chim. Acta. v.257 Ethanol metabolism, cirrhosis and alcoholism Leiber, C.S.
  4. Fed. Proc. v.41 Distribution of stable acetaldehyde adducts in blood under physiological conditions Lumeng, L.;Minter, R.;Li, T.K.
  5. Gastroenterology v.103 Immunoglobulin a antibody to a 200 kilodalton cytosolic acetaldehyde adduct on alcoholic hepatitis Koskinas, J.;Kenna, J.G.;Bird, G.L.;Alexander, G.J.;Williams, R.
  6. Autoimmune manifestation of alcoholic liver diseases Zetterman, R.K.;Kravitt, K.I.;Wiesner, R.H.(eds.)
  7. Hepatology v.12 Acetaldehyde and alcoholic fibrogenesis; Fuel to the fire, but not the spark Friedman, S.L.
  8. Korean resources plant v.3 Kim, T.J.
  9. 中藥大辭典(下券) 江蘇新醫學院(編)
  10. Yakugaku Zasshi v.109 Pharmacological studies on Puerariae Flos. I. The effect of Puerariae Flos on alcoholic metabolism and spontaneous movement in mice Niiho, Y.;Yamazaki, T.;Nakajima, Y.;Itoh, H.;Takeshita, T. Kinjo, J.;Nohara, T.
  11. M.S. Thesis Lowering effects of the flower of Pueraria lobata on blool ethanol levels Sun, M.S.
  12. Yakugaku Zasshi v.110 Pharmacological studies on Puerariae Flos. II. The effect of Puerariae Flos on alcohol-induced unusual metabolism and experimental liver injury in mice Niiho, Y.;Yamazaki, T.;Nakajima, Y.;Itoh, H.;Takeshit, A.T.;Kinjo, J.;Nohara, T.
  13. J. Pharm. Pharmacol. v.49 Pharmacological studies on Puerariae Flos III, Protective effect of kakkalide on ethanol-induced lethality and acute hepatic injury in mice Yamazaki, T.;Nakajima, Y.;Niiho, Y.;Hosono, T.;Kinjo, J.
  14. Phytochemistry v.51 Isoflavone glycosides from the flower of Pueraira lobata Park, H.J.;Park, J.H.;Moon, J.O.;Lee, K.T.;Jung, W.T.
  15. Kor J. Pharmacogn. v.20 Isoflavonoids of Belamcanda chinensis Lee, S.O.;Woo, W.S.;Woo, E.H.;Kim, K.S.
  16. Chem. Parm. Bull. v.47 HPLC profile analysis of hepato protective oeanene-glucuronides in Puerariae Flos Kinjo, J.;Aoki, K.;Okawa, M.;Shii, Y.;Hirakawa, T.;Nohara, T.;Nakajima, Y.;Yamazaki, T.;Niiho, Y.;Kurashige, T.
  17. 2000 Years of Natural Products Research Isolation of new flavone glycosides and antidiabetic principles from the flowers of Pueraria thunbergiana Park, H.J.;Han, S.J.;Lee, K.E.;Kim, D.H.;Choi, J.W.;Lee, H.K.
  18. Biochem. Pharmacol. v.24 Effect of ethanol on hepatic microsomal drug metabolizing enzymes in the rat Liu, S.J.;Ramsey, R.K.;Fallon, H.J.
  19. J. Nutr. v.107 Report of the American Institute of NUtrition, Ad Hoc committee on standards for nutritional studies American Institute of Nutrition
  20. J. Biol. Chem. v.266 A simple method for the isolation and purification of total lipids from animal tissue Folch, J.;Lees, M.;Sloane-Stanley, G.H.
  21. Clin. Chem. v.29 Peroxidase coupled method for the colorimetric determination of serum triglycerides McGrowan, J.D.;Artiss, D.R.;Strandbergy, A.
  22. Clin. Chem. v.18 Estimation of concentration of low density lipoprotion cholesterol in plasma without use of the preparative ultracentrifuge Fridewald, W.T.;Levy, R.I.;Fedreicson, D.S.
  23. J. Biol. Chem. v.239 The carbon mono oxide binding pigments of liver microsomes in evidence for its hemoprotein nature Omura, T.;Sato, R.
  24. Biochim. Pharmacol. v.31 Mutiple drug metabolism P-Nitro anisole reversal of acetone enhanced aniline hydroxylation Bidlack, W.R.;Lowery, G.L.
  25. J. Biol. Chem. v.55 The colorimatric estimation of formaldehyde by means of the hentisch reaction Nash, T.
  26. J. Biol. Chem. v.237 Hepatic aldehyde oxidase, purification and properties Rajagopalan, K.V.;Fridovich, I.;Handler, P.
  27. J. Biol. Chem. v.244 The regulation of rat liver xanthine oxidase : Conversion in vitro of the enzyme activity from dehydrogenase (Type D) to oxidase (Type O) Stirpe, F.;Della, C.E.
  28. Biochem. Biophys. Res. Commun. v.74 Isolation and characterization of an anodic from of human liver alcohol dehydrogenase Borson, W.F.;Li, T.K.;Lange, L.G.
  29. Science v.162 Ethanol oxidation by hepatic microsome : Adaptive increase after ethanol feeding Lieber, C.S.;DeCarli, L.M.
  30. In Methods of enzymatic analysis v.2 Catalase Aebi, H.;Vergmeyer, M.U.(ed.)
  31. Biochem. Behav. v.13 Correlation between human erythrocyte aldehyde dehydrogenase activity and sensitivity to alcohol Inoue, K.;Fukunaga, M.;Yamasawa, K.
  32. J. Biol. Chem. v.193 Protein measurement with folin phenol reagent Lowry. O.H.;Rosebrough, N.J.;Farr, A.L.;Rendall, R.J.
  33. Gastroenterology v.106 Alcohol and the liver Lieber, C.S.
  34. Pharmacology v.7 The energy cost of the matabolism of drugs including ethanol Pirola, R.;Lieber C.S.
  35. Bail. Clin. Gastroent. v.7 Aetiology and pathogenesis of alcoholic liver disease Lieber, C.S.
  36. Lancet v.2 Clinical and metabolic study of alcoholic hyperlipidemia Chiat, A.;February, A.W.;Mancini, M.;Lewis, B.
  37. Korean J. Nutr. v.32 The role of fatty acid bindign protein in the fatty liver induced by alcohol or high cholesterol diet in rats Kim, H.K.;Kwon, Y.A.;Pho, S.R.;Hahm, Y.T.
  38. J. Clin. Invest v.49 Effects of chronic ethanol feeding on serum lipoprotein metabolism in the rat Baraona, E.;Lieber, C.S.
  39. Korean J. Nutr. v.27 A comparative study on serum lipid levels in normals and chronic alcoholics Kim, M.H.;Choi, M.K.
  40. Alcohol v.25 Abnormal low density lipoproteins composition in some chronic alcoholics : A possible mechanism Naruszewicz, M.;Mirkiewicz, W.;Wehr, H.
  41. Am. J. Clin, Nutr. v.23 Physiological role of lecithin cholesterol-acyltranferase Glomset, J.A.
  42. Emzyme v.43 Effect of alcohol on lipoprotein metabolism II. Lipolytic activities and mixed function oxidase Parkes, J.G.;Auerbach, W.;Goldberg, D.M.
  43. Cancer Res. v.44 Changes in molecular forms of rat hepatic glutathione S-transferase during chemical hepatocarcinogenesis Kitahara, A.;Satoh, K.;Nishimura, K.;Ishikawa, T.;Ruike, K.;Sato, K.;Tsuda, H.;Ito, N.
  44. Gastroenteroloty v.62 A quantitative morphological analysis of ethanol effect upon rat liver Dobbin, W.O.;Rollins, E.L.;Brooks, S.G.;Fallin, H.J.
  45. Life Sci. v.9 Drug metabolism in ethanol-induced fatty liver Ariyoshi, T.;Takabatake, E.;Remmer, H.
  46. Gastroenterology v.62 Antioxidant status and alcohol-related disease Ramsey, R.K.;Fallon, H.
  47. Annu. Res. Pharmacol. Tosicol. v.19 Presystemic drug eliminaton Routledge, P.A.;Shand, D.G.
  48. Mol. Pharmacol. v.3 Spectral studies of drug interaction with hepatic microsomal cytochrome Schenkman, J.B.;Remmer, H.;Estabrook, R.W.
  49. Pusan Bull. Pharm. Sci. v.27 Cytochrome P-450 induced lipid peroxidation and oxygen consumption of the liver Moon, J.O.
  50. Biochem. Biophys. Res. Commun. v.143 Acetaldehyde-mediated hepatic lipid peroxidation role of superoxide and ferritin Shaw, S.;Jayatilleke, E.
  51. Gastroenterology v.98 Role of xanthine oxidase on ethanol-induced lipid peroxidation on rats Shinzo, K.;Tateo, K.;Jeffrey, A.;Nobuhiro, I.;Lieber, C.S.
  52. Arch. Biochem. Biophs. v.15 Aldehyde oxidase from rabbit liver : Speciticity toward purines and their analogs Hall. W.H.;Krenitsky, T.A.
  53. Biochem. J. v.268 The role of aldehyde oxidase in ethanol-induced hepatic lipid peroxidation in the rat Shaw, S.;Jayatilleke, E.
  54. Biochem. Pharmacol. v.24 Effects of long-term ethanol treatment on aldehyde and alcohol dehydrogenase activities in rat liver Koivula, T.;Lindros, K.O.
  55. Ph.D. Dissertation Study on the Puffer fish Fugu xanthopterus water extract of detoxification mechanism in subacute alcohol-treated rats Kim, D.H.
  56. Alcohol-Alcoholism v.25 Ethanol induced lipid peroxidation and oxidative stress in extrahepatic tissues Nordmann, R.;Ribiere, C.;Rouach, H.