한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제29권1호
  • /
  • Pages.49-56
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  • 2000
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  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

Bacillus sp. JK-43의 Cyclodextrin Glucanotransferase에 의한 2-O-$\alpha$-D-Glucopyranosyl L-Ascorbic Acid 생산에 관한 연구

Production of 2-O--$\alpha$-D-Glucopyranosyl L-Ascorbic Acid by Cyclodextrin Glucanotransferase from Bacillus sp. JK-43

  • 발행 : 2000.02.01
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초록

The 2-O-$\alpha$-D-glucopyranosyl L-ascorbic acid (AA-2G) which was enzymatically glucosylated with the cyclodextrin glucanotransferase (CGTase) [EC 2.4.1.19] from Bacillus sp. JK-43 has been reported previously. The presnet experiments examined the optimal conditons for the productio of AA-2G from AA and soluble starch, and characterized the properties of the CGTase from Bacillus sp. JK-43. The reaction mixture for the maximal production of AA-2G was followings; 12% total substrate concentration, 1,400 usits/mL of CGTase and a mixing ratio of 2 : 3(g or AA : g of soluble starch). Under this condition, 1.76mM of AA-2G, which corresponded to 2.53% yield based on AA, was produced after incubation for 24hrs at 45$^{\circ}C$ (pH 5.5). The optimum pH and temperature for the CGTase activity were 6.0 and 45$^{\circ}C$, respectively. The enzyme was stable at pH 5.5 to 9.5, and at temperature up to 5$0^{\circ}C$. The thermostability of the enzyme could be enhanced up to 6$0^{\circ}C$ by the addition of 30mM CaCl2.

키워드

참고문헌

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