Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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A Study on the Mutagenicity of Thermally Oxidized Safflower Oil

가열산화 홍화유의 돌연변이원성에 관한 연구

  • 안명수 (성신여자대학교 식품영양학과) ;
  • 이진영 (성신여자대학교 식품영양학과)
  • Published : 2000.02.01
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Abstract

Deep-fat frying is a common cooking practice. There has been considerable concern regarding the mutagenic and carcinogenic potential of thermally oxidized oils. Studies on deep-fried foods so far have revealed not much on the mutagenicity of the oils in the foods. Therefore, in the present study, it was attempted to investigate the mutagenicity ofthe thermally oxidized safflower oil. Oil was heated in a home-fryer at a temperature of 180$\pm$3$^{\circ}C$ for 48 hours. Oil samples were taken at 0, 8, 16, 24, 32, 40 and 48 hours of heating, respectively. Each sample was used to study the changes in peroxide value (POV), acid value (AV), iodine value (IV), conjugated dienoic acid (CDA) content, %, and fatty acid composition. Another series of samples were fractionated into non-polar and polar fractions by column chromatography. The mutagenicity of the samples taken from the thermally oxidized oils, as well as the non-polar and polar fractions of the thermally oxidized oils, was investigated with the Ames test. The Ames test was carried out with and without metabolic activation. Bacterial tester strains used in the present study were the histidine auxotrophic strains of Salmonella typhimurium TA100, TA1535 and TA102 were used for the detection of base pair mutations, and TA98 and TA1537 for frame shift mutations. Each series of samples was dissolved in tetraphydrofuran (inhibitor-free) and tested at doses ranging from 0.05 to 5 mg/plate. None of the oil samples taken during the 48 hour oxidation period showed any mugagenic activity. This was the case, even after the activaton with 59 mix. Also, none of the polar and non-polar fractions showed any mutagenic activity on all the strains tested.

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References

  1. 한국환경성돌연변이 발암원학회지 v.1 암의 원인과 환경 윤택구
  2. Cancer Res. v.52 The lessons of life : Keynote address to the nutrition and cancer conference Doll, R.
  3. J. Nat. Cancer Inst. v.66 The cause of cancer, Quantitative estimates of avoidable risks of cancer in the United States today Doll, R.;Peto, R.
  4. Cancer Res. v.43 Mutagens-carcinogens in foods Sugimura, T.;Sato, S.
  5. J. Nat. Can. Inst. v.43 Induction of intestinal and urinary bladder neoplasms induced by feeding bracken fern (pteris aquilina) Pamukcu, A.M.;Price, J.M.
  6. J. Nutr. v.93 Longterm rat feeding study with used frying fats Nolen, G.A.;Alexander, J.C.;Artman, N.R.
  7. J. Toxicol. Environ. Health v.7 Chemical and biological properties related to toxicity of heated fats Alexander, J.C.
  8. JAOCS v.42 A long-term nutritional study with fresh and mildly oxidized vegetable and animal fats Kaunitz, H.;Johnson, R.E.;Pegus, L.
  9. J. Nutr. v.118 Mitochondrial function in rats is affected by modification of membrane phospholipids with dietary sardine oil Yamaoka, S.;Urade, R.;Kito, M.
  10. J. Nutr. v.118 The effect of dietary essential fatty acid deficiency on the composition and properities of the liver microsomal membrane of rats Chirston, R.;Fernandez, Y.;Cambon-Gros, C.;Periquet, A;Deltour, P.;Leger, C.L.;Mitjavila, S.
  11. Carcinogenesis v.8 Dietary fat regulation of the association of protein kinase C activity with epidermal cell membranes Donnelly, T.E.;Birt, D.F.;Sittler, R.;Anderson, C.L.;Choi, M.;Julius, A.D.
  12. Food Chem. Toxic. v.22 Ames mutagenicity tests of repeated heated edible oils van Gastel, A.;Mathur, R.;Roy, V.V.;Rukmini, C.
  13. Dt. LebensmittRundsch v.76 Untersuchungen von fritierfetten auf mogliche mutagene wirkungen mit hilfe des Ames-tests Scheutwinkel-Reich, M.;Ingerowski, G.;Stan, H.J.
  14. Mutation Research v.204 Assessment of mutagenic activity of repeatedly used deepfrying fats Hageman, G.;Kikken, F.;Hoor, T.;Kleinjans, J.
  15. Mutat. Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D.M.;Ames, B.M.
  16. AOCS : Official Methods and Recommended Practices (4rd ed.)
  17. 基準油脂分析試驗法 v.2 no.4
  18. 基準油脂分析試驗法 v.2 no.3
  19. AOCS : Official Methods and Recommended Practices (4rd ed.)
  20. J. Lipid Res. v.27 Direct transesterification of classes of lipids in a one-step reaction Legage, G.;Roy, C.C.
  21. JAOAC v.64 Chromatographic separation of polar and nonpolar components of frying fats Waltking, A.E.;Wessels, H.
  22. Lipids v.15 Microbiological studies investigating mutagenicity of deep frying fat fractions and some of their components Scheutwinkel-Reith, M.;Ingerowski, G.;Stan, H.J.
  23. Food Chem. Toxicol. v.20 Lack of mutagens in deep-fat fried foods obtained at teh retail level Taylor, S.L.;Berg, C.M.;Shoptaugh, N.H.;Scott, V.N.
  24. J. Food Sci. v.47 Effect of heating temperature and time on the volatile oxidative decomposition of linolenate Lomanno, S.S.;Nawarm W.W.
  25. Food Technol. v.45 Methods for measuring changes in deepfat frying oils Pamela, J.W.
  26. Food Chem. Toxic. v.22 Ames mutagenicity tests of repeated heated edible oils Gastel, A.;Van, Mathur, R.;Roy, V.V.;Rukmini, C.
  27. JAOCS v.60 Mutagen formation in deep-fat friend foods as a function of frying conditions Taylor, S.L.;Berg, C.M.;Shoptaugh, N.H.;Traisman, E.
  28. Food Cosmet. Toxicol. v.18 Mutagenicity of peanut oils and effect of repeated cooking Fong, L.Y.Y.;Ton, C.C.T.;Koonanuwatchaidet, P.
  29. Linoleic acid의 항돌연변이 및 항암효과 임선영
  30. Agric. Biol. Chem. v.43 Mutagenic acitivity of autoxidized linolenic and linoleic acid Yamaguchi, T.;Yamashita, Y.