한국식품영양과학회지 (Journal of the Korean Society of Food Science and Nutrition)

  • 제29권1호
  • /
  • Pages.168-173
  • /
  • 2000
  • /
  • 1226-3311(pISSN)
  • /
  • 2288-5978(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지

전자파를 조사한 흰쥐 간조직에서의 Mixed Function Oxidase System과 Xanthine Oxidase 활성에 미치는 녹차의 영향

Effect of Green Tea on Mixed Functon Oxidase System and Xanthine Oxidase Activities in Rat Liver Exposed to Microwaves

  • 이순재 (대구효성가톨릭대학교 식품영양학과) ;
  • 이용희 (한동대학교 선린병원 신경과)
  • 발행 : 2000.02.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of this study was to investigate the changes of microsomal mixed function oxidase (MFO) and xanthine oxidase activities (XOD) in the liver of rats exposed to microwave. Sprague-Dawley male rats weighing 200$\pm$10g body weight were randomly assigned to a normal and microwave exposed(MW) groups; microwave exposed groups were divided into two groups; microwave (MW) group and green tea(GT) gropu which were fed distilled water and green tea extracts during experimental periods, respectively. Rats were irradiated with microwave at the frequency of 2.45 GHz for 15min and rats wre sacrificed at the 4th day of the microwave irradiaton. The hemoglobin level of GT group was higher than that of the normal gropu and MW group, but the hematocrit value was not significantly different among all experimental gropus. The activity of serum GOT of MW group was significantly increased but that of GT group was similar to normal group. Activities of GPT were not significantly different among all experimental groups. Liver XOD activity was significantly increased in the microwave exposed groups but green tea normalized the XOD activity. The activity of hepatic microsomal cytochrome P450 was significantly increased in MW group compared to normal group and that of GT group was similar to that of the normal group. The activity of hepatic microsomal NADPH-cytochrome P450 reductase was also significantly increased in MW group compared to normal group, but that of GT group was similar to that fo the normal group. In conclusion, the activities of MFO and XOD were elevated by microwave irradiaton, but the activation of MFO system as well as the damage of the liver by microwave were reduced by green tea supplementation.

키워드

참고문헌

  1. Acta Physiol. Scand. v.492 Free radicals as mediators of tissue injury Del Maestro, R.;Thaw, H.H.;Bjork, J.;Planker, M.;Arfors, K.E.
  2. Am. J. Epidemiol. v.134 Occupational exposure to electromognetic fieds and breast cancer in men Demers, P.A.;Thomas, D.B.;Rosenblatt, K.A.
  3. Bioelectromagnetics v.12 Astrocytoma risk related to job exposure to electric and masnetic fields Mack, W.;Preston Martin, S.;Peter, J.M.
  4. Acta Ophthal. (Kbh) v.51 Lenticular and retinal changes secondary to microwave exposure Albert, E.;Tengroth, B.
  5. IEEE Spectrum v.18 Electromagnetic hazard. RF radiation: Biological effects Lerner, E.J.
  6. Biological effects of microwaves. Barandi, S.;Czerski, P.
  7. J. Biochem. v.131 Characterization of alternative molecular forms of xanthine oxidase in the mouse Duke, E.J.;Joyce, P.;Ryan, J.P.
  8. Am. J. Biochem. v.49 The role of the liver in drug metabolism Remmer, H.
  9. Kor. J. Gerontol. v.7 Cytochrome $P_{450}$ contents and NADPH-cytochrome $P_{450}$ reductase of liver in rat exposed to microwaves Choi, J.H.;Rhee, S.J.;Kim, J.H.;Kim, S.Y.
  10. J. Nutr. Sci. Vitaminol. v.32 Effect of green tea catechins on plasma cholesterol-fed rats Muramatsu, K.;Fukuyo, M.;Hara, Y.
  11. International symposium on tea science(Shizuoka). Tea catechins decrease micellar solubility and intestinal absorption of cholesterol in rats. Ikeda, I.;Masato, Y.;Nakayama, M.;Takeo, T.;Yayabe, F.;Sugano, M.
  12. Jap. J. Cancer Res. v.80 Inhibitory effect of (-)-epigallocatechin gallate on carcinogenesis with N-ethyl-N'-nitro-N-nitrosoguanidine in mouse duodenum Fujita, Y.;Yamane, T.;Tanaka, M.;Kuwata, K.;Okuzumi, J.;Takehashi, T.;Fujiki, H.;Okuda, T.
  13. Acta Experimental Biology v.9 The preliminary study of inhibitory effects of green tea antioxidant on mutation Cheng, S.J.
  14. Oyo. Yakuri. v.40 Pharmacological action of tea extract on the central nervous system in mice Hayashi, E.;Hayashi, M.;Yamazoe, H.
  15. Mutation Res. v.150 Detection and chemical identification of natural bioantimutagens Kada, T.;Kaneko, K.;Matsuzaki, S.;Matsuzaki, T.;Hara, Y.
  16. Nippon Shokuhin Kogyo Gakkaishi v.38 Platelet aggregation inhibitory activity of tea extracts Kazuko, N.;Midor, Y.;Chikusa, T.;Michido, I.;Mitsuo, N.
  17. Nippon Nogeikagaku Kaishi v.59 Antioxidative activity of tea leaf catechins Matsuzaki, T.;Hata, Y.
  18. J. Korean Soc. Food Sci Nutr. v.26 Effect of green tea catechin on cytochrome P, xanthine oxidase activities in liver and liver damage in streptozotocin induced diabetic rats Park, G.Y.;Rhee, S.J.;Im, J.G.
  19. School of Food and Nutritional Science, University of Shizuoka, Japan, 7, 1991-1993 Suppression of hydroperoxide-induced cytotoxicity by polyphenols. Nakayama, Y.
  20. Clinical Diagnosis by Laboratory Methods Davidshon, E.;Nelson, D.A.
  21. Am. J. Clin. Pathol. v.28 A colorimetric method for the determination of serum glutamic oxaloactic and glutamic pyruvic transaminase Reitman, S.;Frankel, S.
  22. J. Biol. Chem. v.244 The regulation of rat liver xanthine oxidase Stripe, J.;Della Corte, E.
  23. J. Biol. Chem. v.239 The carbon monoxide binding pigment of liver microsomesⅡ. Solubilization, purification and properties Omura, T.;Sato, R.
  24. Meth. Enzymol. v.10 The preparation and properties of microsomal TPNH-cytochrome C reductase from pig liver Masters, B.S.S.;Williams, C.H.;Karmin, H.
  25. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O.H.;Rosenbrouth, N.J.;Farr, A.L.;Randall, R.J.
  26. Korean J. Nutr. v.26 Effect of dietary selenium of metallothionein sybthesis and antioxidative detoxification mechanism in cadmium administered rats Rhee, S.J.;Jun, S.Y.
  27. J. Korean Soc. Food Sci. Nutr. v.25 Effect of dietary vitamin E on the microsomal mixed function oxidase system of liver and lung in streptozotocin-in-duced diabetic rats Park, Y.R.;Rhee,S.J.;Lim, Y.S.;Joo, G.J.
  28. J. Biol. Chem. v.261 Preparation and characterization of FAD-dependent NADPH-cytochrome $P_{450}$ reductase Gary, P.K.;Henry, W.S.