Saikosaponin Contents and cAMP Phosphodiesterase Inhibitory Activities in Bupleuri Radix Extracts According to Extraction Conditions

추출조건에 따른 시호건근 추출액 중의 Saikosaponin함량 및 cAMP Phosphodiesterase 저해활성

Two major oleananesaponins in Bupleuri radix, saikosaponin $\alpha$ and d, were extracted at various solvent compositions and leaching temperatures. Solvent composition was varied at 0 to 100%(v/v) of ethanol-water and methanol-water, whereas leaching temperature was adjusted to te range of 25~45$^{\circ}C$. The most effective extractant and leaching temperature were 70% ethanol and 45$^{\circ}C$, respectively. However, no major differences in saikosaponin content and cAMP phosphodiesterase inhibition were found at various leaching times. The cAMP phosphodiesterase inhibitions were alo the highest when 70% ethanol was used as the extractant.