Journal of Nutrition and Health

  • 제33권2호
  • /
  • Pages.167-178
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  • 2000
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  • 2288-3886(pISSN)
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  • 2288-3959(eISSN)
한국영양학회 (The Korean Nutrition Society)
권호 표지

한국 남자 흡연자의 금연과 항산화제 보충에 따른 체내 가역적.비가역적인 산화 손상도 변화의 정량적 측정 연구

The Quantitative Determination of Reversible and Irreversible Oxidative Damages Induced by Smoking Cessation and Supplementation of Antioxidative Vitamins in Korean Male Smokers

  • 김미경 (국민대학교 사범대학 가정교육과)
  • 발행 : 2000.03.01
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초록

The effect of oral vitamin e (800IU/day) and C (500mg/day) supplementation for 10 days and/or smoking cessation for 5 days on oxidative damage to the red blood cells (RBC) of male smokers (22.2$\pm$0.2 years old) was studied. RBC were tested for their ability to protect against smoking-induced oxidative damage by measuring heme proteins (carboxyhemoglobin, hemoglobin, methemoglobin, oxyhemoglobin), hemolysis and thiobarbiturinc acid reactive substances (TBARS). Plasma levels of vitamin c, A, E, $\beta$-catotene, total cholesterol, glutamic pyruvic transaminase(GPT) and glutamic oxaloacetic transaminase(GOT) were also analyzed. In experiment one, a comparison was made of heme proteins and lipid damage to RBC, plasma antioxidant status (indexed by plasma levels of vitamin C, E, A and $\beta$-carotene) between smokers(n=56) and non-smokers (n=16). No differences were found in plasma antioxidant status, heme protein damage and TBARS concentration of RBC. In experiment two, 46 fasting male smokers from experiment one were divided into 4 groups. The groups were smoking with placebo group(SP, n=14), smoking cessation with vitamins supplementatin group (SV, n=13), smoking cessation with placebo group (NSP, n=9) and smoking cessation with vitamins supplementation group (NSV, n=10). After supplementing antioxidant vitamins, significant increases were seen in plasma vitamins supplementation group (NSV, n=10). After supplementing antioxidant vitamins, significant increases were seen plasma vitamin C (p<0.05) and vitamin E levels (p<0.05). The plasma vitamin E level was highest in the NSV group. Vitmain E and C supplementation provided some protection against heme proteins and lipid damage by lowering methemoglobin, hemolysis and TBARS concentration of RBC. Smoking cessation significantly decreased TBARS of RBC and plasma total cholesterol concentration. Supplementing vitamin E and C with smoking cessation considerably lowered plasma total cholesterol. These results point to a special association among smoking, oxidative damage and plasma antioxidant vitamin status. They indicate that increases in plasma antioxidant status can be detected after the supplementation of vitamin C and E and that smoking cessation had an additional effect on plasma vitamin E level. The present data suggest that improved antioxidant status induced by antioxidant supplementation or smoking cessation may help prevent oxidative damage in smokers.

키워드

참고문헌

  1. Biol Nutr Diet v.37 On The antioxidant hypothesis with regard arteriosclerosis Gey KF
  2. Proc Natl Acad Sci USA v.90 Oxidants, antinomians and the degenerative diseases of aging Ames BN;Shigenaga MK;Hagen TM
  3. Eur J Biochem v.215 Strategies of antioxidative defense Sies H
  4. Haemostasis v.23 no.suppl The role of oxygen radicals in human disease with particular reference to the vascular system Halliwell B
  5. J Biol Chem v.262 Age-related changes in oxidized proteins Oliver CN;Ahn B-W;Moerman EJ;Goldstein S;Stadtman ER
  6. Lancet v.344 Free radicals and antioxidants and human disease, curiosity cause, or consequence Halliwell B
  7. Free Rad Res Commun v.7 Endogenous oxidative DNA damage, aging and cancer Ames BN
  8. Biochem Biophys Res Commun v.19 Inherited enhancement of hydroxyl radical generation and lipid peroxidation in the SD strain rats results in DNA rearrangements, degenerative disease and premature aging Salganik RI;Solovyva NA;Kikalov Si;Grishaeve ON;Memenova LA;Popovsky AV
  9. Nature v.283 Cigarette-smoke induce DNA damage and lung cancer risks Hopkin JM;Evans HJ
  10. Ann NY Acad Sci v.686 Cigarette smoke-mediated oxidant stress, phagocytes, vitamin C,vitamin E and tissue injury van Antwerpen L;Theron AJ;Myer MS;Richards GA;Wolmarans L;Booysen U;van der Merwe CA;Sluis Cremer GK;Anderson R
  11. Atherosclerosis v.85 Effect of vitamin C and E supplementation on susceptibility of plasma lipoproteins to peroxidation induced by acute smoking Harats D;Ben-Naim M;Dabach Y;Hollander G;Havivi E;Stein O;Stein Y
  12. J Nutr Biochem v.7 Acute effects of smoking cessation on antioxidant status Brown A
  13. Free Rad Biol Med v.9 Malondialdehyde and thiobarbituric acid reactivity as diagostic indices of lipid peoxidation and oxidative tissue injury Janero DR
  14. J Nutr Biochem v.6 Protection by vitamin E, selenium, trolox, ascorbic acid palmitate,acetylcysteine, coenzyme Q, β-carotene and (+)-cathechin against oxidative damage to rat liver and heart tissue slices measured by oxidized heme proteins de Mulder CLC;Madabush HT;Tappel AL
  15. Free Rad Biol Med v.15 Ferrous-iron-induced oxidation in chicken liver slices as measured by hemichrome formation and thiobarbituric acid-reactive substances: Effects on dietary vitamin E and β-carotene Anderson HJ;Chen H;Pellet LJ;Tappel AL
  16. Environ Health Perspective v.64 Free radical production and oxidative reaction of hemoglobin Winterbourn CC
  17. Korean J Nutr v.3 A study on development and validation of food frequency questionnaire for Korean Kim WY;Yand EJ
  18. Biochem Biophys Acta v.1037 Oxidant induced haemoprotein degradation in rat tissue slices: effect of bromotrichloromethane, antioxidants and chelators Hildago FJ;Zamora R;Tappel AL
  19. Ann NY Acad Sci v.686 Oxidants in cigarette smoke Pryor QA;Stone K
  20. J Nutr Sci Vitaminol v.27 Separative determination of ascorbic acid and erythorbic acid in animal tissues by high-performance liquid chromatography Otsuda M;Kurota T;Suzuki E;Arakawa N;Inagaki C
  21. Am J Clin Nutr v.32 Simultaneous determination of α-tocopherol and retinol in plasma or red cells by high pressure liquid chromatography Bieri JG;Tolliver TJ;Catignani GL
  22. Arch Biochem Biophys v.294 Cis-trans isomers of lycopene and β-carotene in human serum tissues Stahl W;Schwarz W;Sundquist AR;Sies H
  23. Methods in Enzymol v.52 Microsomal lipid peroxidation John AB;Steven DA
  24. Recommended dietary allowances for Korean(6th ed.) The Korean Nutrition Society
  25. J Epidemiol Commun Health v.45 Relationship between diet and smoking-Is the diet of smokers different? Cade JE;Margetts BM
  26. J Chron Dis The effect of cessation of smoking on body weight in 57,032 women: cross-sectional and longitudinal analysis Blitzer PH;Rimm AA;Giefer EE
  27. Arch Environ Health v.20 Metabolic changes associated with the cessation of cigarette smoking Glauser SC;Gause EM;Reidenberg MM;Rusy BE;Tallarida RJ
  28. JAMA v.112 The effects of tobacco smoking on the alimentary canal Newsholme JC;IVy Ac
  29. Ann NY Acad Sci v.686 Antioxidant vitamin intakes in Scottish smokers and non-smokers Bolton Smith C
  30. Korean J Nutr v.29 Vitamin C intakes and serum levels in smoking college students Park JA;Kang MH
  31. Br J Preven Soc Med v.26 Obesity and smoking habits by social class Khosla T;Lowe CR
  32. Korean J Nutr v.24 Estimation of dietary fiber intake of college students Lee HS;Lee YK;Chen SC
  33. Am J Clin Nutr v.40 Diet, smoking social class and body mass index in the coronary heart disease study Fehily AM;Phillips KM;Yarnell WG
  34. J Am Diet Assoc v.78 Nutrition data collection in the Multiple Risk Factor Intervention Trial(MRFIT) Tillotson J;Gorder D;Kassim N
  35. Am J Clin Nutr v.41 The relation of drinking and smoking habit to diet: the lipid research clinics prevalence study Fisher M;Gordon T
  36. Am J Clin Nutr v.35 Smoking and its effects on the systems of caloric regulation Wack JT;Rodin J
  37. JAMA v.263 Dose-related effects of cigarette smoking on olfactory function Fryer RE;Schwartz BS;Dorty RL
  38. Am J Public Health v.79 no.2 The influence of smoking on vitamin C status in adults Schectman G;Byrd JC;Gruchow HW
  39. Cancer Res v.45 Serum vitamin levels and the risk of cancer of specific sites in men of Japanese ancestry in Hawaii Nomura AMY;Stammermann GN;Heibrun LK
  40. Ann NY Acad Sci v.498 Requirement for vitamin C based on metabolic studies Kallner AB
  41. Am J Epidemiol v.127 The Relationship of diet, cigarette smoking and alcohol consumption to plasma beta-carotene and alpha-tocopherol levels Stryker WS;Kaplan LA;Stein EA;Stempfer MJ;Sober A;Willett WC
  42. Korean J Nutr v.31 Effects of vitamin C supplementation on immune status in smoking and nonsmoking male college students Kim WK
  43. Am J Clin Nutr v.59 Effect of smoking on serum vitamin concentrations in African-American women Pamuk ER;Byers T;Coates RJ;Vann JQ;Sowell AL;Gunter EW;Glass D
  44. Int J Vitam Nutr Res v.16 no.suppl Plasma vitamin C levels in smokers Hoefel OS
  45. Eur J Clin Nutr v.43 Dietary intake and other determinant of blood vitamins in an elderly population Herberth B;Chavnace M;Musse N;Mejean L;Bemhes F
  46. Am J Cin Nutr v.42 Effect of smoking and vitamin E on blood antioxidant status Durthie GG;Arthur JR;James WPT
  47. Am J Clin Nutr v.65 Erythorocyte vitamin E and plasma ascorbate concentrations in relation to erythrocyte peroxidation in smokers and non smokers: dose response to vitamin E supplementation Brown KM;Morrice PC;Duthie GG
  48. Chem Phys Lipids v.45 Lipid peroxidation in erythrocytes Clemens RC;Waller HD
  49. Br Med J v.28 Cigarette smoking and serum lipid and lipoprotein: an analysis of published data Craig WY;Palomaki GE;Haddow JE
  50. Ann NY Acad Sci v.498 Serum levels of vitamin C in relation to dietary and supplemental intake of vitamin C in smokers and nonsmokers Smith JL;Hodeges RE
  51. J Nutr v.121 Comparative antioxidant effectiveness of dietary β-carotene, vitamin E,selenium and coenzyme $Q_{10}$ in rat erythrocytes and plasma Zamora R;Hildago F;Tappel AL
  52. Atheroscler Thomb Vascular Biol v.15 Supplementation with low dose vitamin E protects LDL from lipid peroxidation in men and women Prince HMG;Duyvenvorde W;Butnh R
  53. Am J Clin Nutr v.60 Vitamin E Supplementation suppresses indexes of lipid peroxidation and platelet counts in blood of smokers and nonsmokers but plasma lipoprotein concentrations remain unchanged Brown KM;Morrice PC;Duthie GG
  54. Atherosclerosis v.112 Vitamin E, C, and lipid peroxidation in plasma and arterial tissue of smokers and non-smokers Mezzetti A;Lepenna D;Pierdomenico SD;Calafiore Am;Costantini F;Riario Sforza G;Imbastaro T;Neri M;Cuccurullo F
  55. Am J Cardiol v.77 Usefulness of antioxidant vitamins in suspected acute myocardial-infarction(the Indian experiment of infarct survival-3) Singh RB;Niaz MA;Rastogi SS;Rastogi S