Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Fermentative Production of Succinic Acid from Glucose and Corn Steep Liquor by Anaerobiospirillum succiniciproducens

  • Lee, Pyung-Cheon (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Woo-Gi (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Sang-Yup (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Chang, Ho-Nam (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology) ;
  • Chang, Yong-Keun (Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology)
  • Published : 2000.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Anaerobiospirillum succiniciproducens requires expensive complex nitrogen sources such as yeast extract and polypeptone for its growth and succinic acid production. It was found that A. succiniciproducens was able to grow in a minimal medium containing glucose when supplemented with corn steep liquor (CSL) as the sole complex nitrogen source. The concentration of CSL had a significant effect on the glucose consumption by A. succiniciproducent. When 10-15 g/L of CSL was supplemented, cells were grown to an OD(sub)600 of 3.5 and produced 17.8 g/L succinic acid with 20 g/L glucose. These results are similar to those obtained by supplementing yeast extract and polypeptone, thereby suggesting that succinic acid can be produced more economically using glucose and CSL.

Keywords

References

  1. Biotechnol. Prog v.15 Catalytic upgrading of fermentation-derived organic acids Sriram, V;J. M. Dennis
  2. Biotechnol. Prog v.15 Biocommodity engineering Lynd, L. R.;C. E. Wyman;T. U. Gerngross
  3. Appl. Biochem. Biotechnol. v.45;46 Methodology for evaluating the economics of biologically producing chemicals and materials from alternative feedstocks Landucci, R.;B. Goodman;C. Wyman
  4. Enzyme Microb. Technol v.24 Succinic acid production by Anaerobiospirillum succiniciproducens;Effects of the H₂/CO₂ supply and glucose concentration Lee, P. C.;W. G. Lee;S. Kwon;S. Y. Lee;H. N. Chang
  5. Process Biochem v.35 Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid producrion Lee, P. C.;W. G. Lee;S. Y. Lee;H. N. Chang
  6. Appl. Biochem. Biotechnol v.57;58 Studies on nutrient requirements and cost-ettective supplements for ethanol production by recombinant E. coli lawford, H. G;J. D. Rousseau
  7. Enzyme Microb. Technol. v.26 Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate Kwon, S;P. C. Lee;E. G. Lee;Y. K. Chang;H. N. Chanh
  8. Appl. Microb. Biotechnol v.47 Development of a low-cost fermentation medium for sthanol production from biomass Kadam, K. L.;M. M. Newman
  9. Appl. Microbiol.Biotechnol. v.54 Batch and continuous fermentation of succinic acid from whey by Anaerobiospirillum succinicprodeucens Lee, P. C.;W. G. LEe;S. Kwon;S. Y. Lee;H. N. Chang