Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Toxicity Monitoring of Endocrine Disrupting Chemicals (EDCs) Using Freeze-dried Recombinant Bioluminescent Bacteria

  • Kim, Sung-Woo (Department of Environmental Science and Engineering and Advanced Environmental Monitoring Research Center, Kwangju Institute of Science and Technology) ;
  • Park, Sue-Hyung (Department of Environmental Science and Engineering and Advanced Environmental Monitoring Research Center, Kwangju Institute of Science and Technology) ;
  • Jiho Min (Department of Environmental Science and Engineering and Advanced Environmental Monitoring Research Center, Kwangju Institute of Science and Technology) ;
  • Gu, Man-Bock (Department of Environmental Science and Engineering and Advanced Environmental Monitoring Research Center, Kwangju Institute of Science and Technology)
  • Published : 2000.11.01
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Abstract

Five different freeze-dried recombinant bioluminescent bacteria were used for the detection of cellular stresses caused by endocrine disrupting chemicals. These strains were DPD2794 (recA::luxCDABE), which is sensitive to DNA damage, DPD2540 (fabA::luxCDABE), sensitive to cellular membrane damage, DPD2511 (katG::luxCDABE), sensitive to oxidative damage, and TV1061 (grpE::luxCDABE), sensitive to protein damage. GC2, which emits bioluminescence constitutively, was also used in this study. The toxicity of several chemicals was measured using GC2. Damage caused by known endocrine disrupting chemicals, such as nonyl phenol, bisphenol A, and styrene, was detected and classified according to toxicity mode, while others, such as phathalate and DDT, were not detected with the bacteria. These results suggest that endocrine disrupting chemicals are toxic in bacteria, and do not act via an estrogenic effect, and that toxicity monitoring and classification of some endocrine disrupting chemicals may be possible in the field using these freeze-dried recombinant bioluminescent bacteria.

Keywords

References

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