Korean Journal of Poultry Science (한국가금학회지)

The Korean Society of Poultry Science (한국가금학회)
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Study on Characteristics of Lactobacillus Isolated from Hen′s Cecum

산란계 맹장 유산균의 특성에 관한 연구

  • 김상호 (축산기술연구소 대전지소) ;
  • 박수영 (축산기술연구소 대전지소) ;
  • 유동조 (축산기술연구소 대전지소) ;
  • 이상진 (축산기술연구소 대전지소) ;
  • 나재천 (축산기술연구소 대전지소) ;
  • 최철환 (축산기술연구소 대전지소) ;
  • 이상진 (축산기술연구소 대전지소) ;
  • 류경선 (전북대학교 동물자원학과)
  • Published : 2000.12.01
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Abstract

Preset study was carried out to evaluate characteristics of lactic acid producing bacteria(LAB) in hen's cecum as probiotics value. Distribution of LAB in intestinal tracts was investigated using 5∼25 weeks - old hens. So, 12 strains to LAB with different morphology were isolated purely. Acid tolerance of LAB tested at pH 1, 2, 3, and 4, and bile resistant also tested at 0, 0.3% and 0.5% bile salt concentration. Growth pattern of LAB observed to 60h. All strains of cecal LAB couldn't survive at pH 1, and decreased linearly survival colony after incubation at pH 2 although some strains could survive for 2h. Most of LAB maintained constant number at pH 3 and 4. The bacterial action could increase linearly at 0% bile salt concentration in all of tested strains. However, only one strain could multiply at 0.3% bile salt, others were influenced by bile salt. That tendency was similar at 0.5% bile salt. Growth was peaked at 12 to 18 h after innoculation. After peak, the decreasing pattern of colony was different to strains which some strains decreased rapidly or maintained for long time. The LAB of hen's cecum was similar to intolerance acidity, but different to resistant to bile salt and growth pattern by strain. So, we choose three strains which have probiocs value, and identified as Lactobacillus amylovorus LLA7, Lactobacillus crispatus LLA9 and Lactobacillus vaginalis LLA11.

Keywords

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