The Korean Journal of Ceramics

The Korean Ceramic Society (한국세라믹학회)
권호 표지

Optical Absorption and Fluorescence of NADH Encapsulated Sol-Gel Silicate Gels

  • Hong, Hye-Jeong (Laboratory of Optical Materials and Coating (LOMC), Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technolog) ;
  • Jiin Jung (Laboratory of Optical Materials and Coating (LOMC), Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technolog) ;
  • Jeong, Ae-Young (Inorganic New Material Chemistry Lab, Department of Fine Chemicals Engineering & Chemistry, Chungnam National University) ;
  • Kim, Dong-Pyo (Inorganic New Material Chemistry Lab, Department of Fine Chemicals Engineering & Chemistry, Chungnam National University) ;
  • Bae, Byeong-Soo (Laboratory of Optical Materials and Coating (LOMC), Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technolog)
  • Published : 2000.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Reduced nicotinamide adenine dinucleotide (NADH) was encapsulated in transparent porous sol-gel silicate gels using by different organoalkoxysilane precursor. Characteristics optical absorption and fluorescence of NADH in the gels were examined with depending on NADH concentration and compared. Optical absorption in the aminopropyltrimethoxysilane (APTMS) gel is highest and remains constant during aging the gel. Thus, it is found that NADH in the APTMS gel is most stable and activated. On the other hand, methyltriethoxysilane (MTES) gel presents the lowest optical absorption diminishing with aging the gel. Measurable increase of fluorescence with raising the NADH concentration is observed except for the APTMS gel due to its solubility in the buffer during fluorescence measurement.

Keywords

References

  1. Chem.Mater v.6 no.10 Enzymes and Other Proteins Entrapped in Sol-Gel Materials D.Avnir;S.Braun;O.Lev;MOttolenghi
  2. Anal. Chem. v.66 no.22 Sol-Gel Encapsulation Methods for Biosensors B.C.Dave;B.Dunn;J.S.Valentine;J.I.Zink
  3. J.Am. Chem.Soc. v.117 no.35 Nicotinamide Adenine;Dinucleotide Phosphate Fluorescence and Absorption Monitoring of Enzymatic Activity in Silicate Sol-Gels for Chemical Sensing Applications S.A.Yamanaka;B.Dunn;J.S.Valentine;J.I.Zink
  4. J.Am.Chem.Soc. v.120 no.18 Sol-Gel-Encapsulated Alcohol Dehydrogenase as a Versatile, Environmentally Stabilized Sensor for Alcohols and Aldehydes A.K.Williams;J.T.Hupp
  5. Principles of Biochemistry A.White
  6. Anal. Chem. v.60 no.10 Fiber-Optic Biosensors Based on the Fluorometric Detection of Reduced Nicotinamide Adenine Dinucleotide J.Wangsa;M.A.Arnold
  7. material Research Society Symposium Preceedings, Vol.3. Better Ceramics Through Chemistry v.3 Organically Modified Silicates by The Sol-Gel Process H.Schmidt;C.J.Brinker(ed.);D.E.Clark(ed.);D.R.Ulrich(ed.)
  8. Biological Spectroscopy I.D.Campbell
  9. J. Photochem. Photobio. B: Biology v.37 no.1-2 Time-resolved and Steady-state Fluorescence Measurement of ?-nicotina-mide Adenine Dinucleotide-alcohol Dehydrogenase Complex during UVA Exposure K.Konig;M.W.Berns;B.J.Tromberg