Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effects of Addition of Exogenous Gonadotropins and/or an Antioxidant to Serum-Free Medium on in vitro Maturation of Bovine Immature Oocytes

무혈청배지에 첨가된 성선자극호르몬 및 항산화제가 소 미성숙난자의 체외성숙능에 미치는 영향

  • Lim, J. M. (Department of Agricultural Biotechnolgoy, College of Agriculture and Life Science, Seoul National University) ;
  • Park, S. E. (College of Medicine, Pochon CHA University) ;
  • Chung, H. M. (College of Medicine, Pochon CHA University) ;
  • Lee, B. C. (College of Veterinary Medicine, Seoul National University) ;
  • Lee, E. S. (Dept. of Veterinary Medicne, Kangwon National University) ;
  • Ko, J. J. (College of Medicine, Pochon CHA University) ;
  • Park, C. (College of Medicine, Pochon CHA University) ;
  • Cha, K. Y. (College of Medicine, Pochon CHA University) ;
  • Hwang, W. S. (College of Veterinary Medicine, Seoul National University)
  • 임정묵 (서울대학교 농업생명과학대학 농생명공학부) ;
  • 박성은 (포천중문의과대학교 의학과) ;
  • 정형민 (포천중문의과대학교 의학과) ;
  • 이병천 (서울대학교 수의과대학) ;
  • 이은송 (강원대학교 수의학과) ;
  • 고정재 (포천중문의과대학교 의학과) ;
  • 박찬 (포천중문의과대학교 의학과) ;
  • 차광렬 (포천중문의과대학교 의학과) ;
  • 황우석 (서울대학교 수의과대학)
  • Published : 2000.12.01
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Abstract

This study was conducted to examine the effects of exogenous gonadotropins (PMSG+hCG) and an antioxidant (cysteine) on in vitro maturation of bovine follicular oocytes. Cumulus-oocyte complexes (COCs) aspirated from 2 to 5 mm ovarian follicles were cultured for 22 to 24 hours in a modified bovine embryo culture medium (mBECM) supplemented with 3 mg/mL bovine serum albumin, to which PMSG (10 IU/mL) + hCG (10 IU/mL) and/or cysteine (0.6 mM) were added. When examined the expansion of cumulus ce1ls at the end of maturation culture, greater (p<0.05) expansion was found after addition of PMSG+hCG (79 to 96%) to mBECM than after no addition (0%), regardless of the presence or absence of cysteine in the medium. The addition of cysteine did not stimulate cumulus expansion, but a high proportion (92%) of expansion was achieved when COCs were cultured after the addition of PMSG+hCG and cysteine to the medium. No difference in the proportion of oocytes underwent germinal vesicle breakdown (initiation of maturation) was found after the addition of PMSG+hCG and/or cysteine to mBECM. However, nuclear maturation (development to the metaphase-II stage) of oocytes was significantly stimulated by the combined addition of PMSG+hCG and cysteine, compared with no addition. In conclusion, both exogenous gonadotropins and an antioxidant are important for nuclear maturation of bovine immature oocytes and these factors have a cell-specific stimulatory action.

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